Effect of intravitreal injection of Conbercept with laser photocoagulation on diabetic macular edema / 国际眼科杂志(Guoji Yanke Zazhi)

· AIM:To analyze the clinical effect of intravitreal injection of Conbercept combined with retinal laser photocoagulation in the treatment of patients with diabetic macular edema (DME).· METHODS:Totally 73 patients (80 eyes) with type 2 diabetes and DME were enrolled in our hospital from June 2015 to December 2016,according to different treatment methods,and they were randomly divided into control group and treatment group.The control group were treated with retinal laser photocoagulation,and the treatment group were treated with intravitreal injection combined with laser photocoagulation.We observed the best corrected visual acuity (BCVA),retinal thickness and complications during the operation before treatment and 3mo after treatment.· RESULTS:At 3mo after treatment,the improvement of BCVA,the decreased value of average retinal thickness and retinal thickness at inferior,superior,temple and nasal in the treatment group were better than those in the control group and those after treatment was better than before(P＜ 0.05).There was no statistically significant difference in the incidence of complications occurrence between the two groups (P＞0.05).None of the patients had severe ocular complications such as corneal edema,anterior chamber inflammatory reaction,retinal hemorrhage,neonatal vascular glaucoma,endophthalmitis,etc.during follow up period.· CONCLUSION:Compared with applying laser photocoagulation alone,intravitreal injection of conbercept combined for DME is more effective with improved visual acuity,restored retinal function,and has good safety.

Adenovirus-mediated delivery of bcl-2 gene attenuates cisplatin-induced degeneration of cultured spiral ganglion cells / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To assess the protection against cisplatin-induced ototoxicity by adenovirus-mediated overexpression of the bcl-2 gene in cultured spiral ganglion cells (SGC).</p><p><b>METHODS</b>SGC from P3 rats were cultured in vitro and exposed to adenovirus vector carrying green fluorescent protein gene (Ad-GFP), followed by immunocytochemical analysis for expression of the neuron-specific marker Neurofilament 200 (NF200) and detection under laser scanning confocal fluorescence microscope. Then, SGC were transduced by Ad-bcl-2 and the expression of human bcl-2 protein was evaluated by Western Blot. Finally, the cultures of SGC were divided into 4 groups: the group of Ad-bcl-2 transfection followed by cisplatin treatment, the group of Ad-GFP transfection followed by cisplatin treatment, the group of cisplatin treatment only and the untreated group. Cisplatin worked for 48 hours at a concentration of 2 microg/ml. Outcome measures included survival number of SGC and longest neurite length by using ImageJ software.</p><p><b>RESULTS</b>SGC were cultured successfully in vitro and transfected by adenovirus vector safely and efficiently. By Western Blot, human bcl-2 protein was expressed in the group after exposure to Ad-bcl-2, but not in the Ad-GFP transfected SGC. Cisplatin exposure resulted in shrinking of neuritis and pyknosis of cell body, even cell death. Expression of bcl-2 in the SGC provided a significant level of protection against cisplatin-induced SGC degeneration.</p><p><b>CONCLUSIONS</b>Our results suggest that SGC can be transduced by adenovirus vector safely and efficiently in vitro. Adenovirus-mediated delivery of the bcl-2 gene attenuates cisplatin-induced SGC degeneration.</p>

Construction of recombinant adenovirus containing human Bcl-2 and its expression in the spiral ganglion cells / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To construct the adenoviral vector containing human Bcl-2 gene and to study the expression of the gene in the spiral ganglion cells (SGC) in vitro.</p><p><b>METHODS</b>Human Bcl-2 cDNA obtained from the plasmid pUC-CAGGS/Bcl-2 was cloned into the plasmid pAdTrack-CMV. Then, pAdTrack/Bcl-2 was cotransferred with adenoviral backbone vector into E. coli strain BJ5183. The recombinant adenoviral plasmid was identified by restriction analysis with Pac I and transfected into HEK293 cells to package and amplify recombinant adenovirus particles which would be identified by Electron microscope. After the adenovirus infected the rat spiral ganglion cells, the expression of Bcl-2 gene was detected by Western Blot and RT-PCR.</p><p><b>RESULTS</b>The recombinant AdEGFP/Bcl-2 plasmid was correctly constructed and confirmed by restriction endonuclease analysis. The viral particles in HEK293 cells were identified by Electron microscope. RT-PCR and Western blot showed that Bcl-2 gene was exactly transcripted and expressed in transgene SGC.</p><p><b>CONCLUSIONS</b>The method based on homologous recombination in bacteria is simple and high efficient. The recombinant adenoviral vector containing human Bcl-2 cDNA was constructed and the transgene SGC expressed human Bcl-2 gene in vitro successfully. It provided foundation for the further study of protection for the impaired SGC by hBcl-2 gene.</p>