Species differences of Baishouwu based on characteristic chromatogram and content determination of acetophenones / 药学实践杂志

Objectives To provide scientific basis for the quality control of different species of Baishouwu by establishing the HPLC fingerprint of domestic of Baishouwu and determining the main active components of acetophenones. Methods HPLC-DAD method was used to determine the HPLC fingerprints of domestic of Baishouwu. Then, the content of 4 kinds of acetophenones in Baishouwu was determined. The column was Diamonsil C18（250mm×4.6mm, 5μm）with the mobile phase of methanol and 0.1% phosphoric acid at a flow rate of 1.0 ml/min. The detection wavelength of p-Hydroxyl acetophenone, baishouwu benzophenone, 2',4'-Dihydroxy acetophenone was set at 260 nm and 2',5'-Dihydroxy acetophenone at 280 nm respectively. Results The similarity and cluster analysis in HPLC fingerprint showed that the constituents were significantly different among C. bungei, C. auriculatum and C. wilfordii. The content of total acetophenones in C. wilfordii was significantly higher than that in other localities of C. auriculatum and C. bungei. Conclusions Acetophenone could be used as the evaluation index to evaluate the quality of Baishouwu in different origins. The content of total acetophenone in C. wilfordii is the highest, which could be used as the best quality resource of Baishouwu.

Anti-tumor activities of endophytes from Cynanchum bungei Decne / 药学实践杂志

Objective To investigate the taxonomic structure and diversity of endophytic fungi from Cynanchum bungei Decne., explore the potential microbial resources and functions and provide the theoretical basis for new antitumor endophytic fungi. Methods The diversities of endophytic fungi community in different tissues, species and habitats were analyzed with traditional endophytic bacteria separation method and 18sRNA high-throughput sequencing technology. MTT assay was used to detect the cytotoxic activity of endophytic fungi from Radix Polygoni multiflori. Results 90 strains of endophytic fungi were isolated and identified from roots, stems, and leaves of C. bungei. Among them, Fusarium and Alternaria were the dominant genera. There were 8, 9 and 13 genera from roots, stems and leaves of C. bungei respectively. Among which Alternaria and Colletotrichum were the common genera in different tissues. Further studies showed that 13 endophytic fungi of C.bungei had good anti-tumor activity in vitro, accounting for 14.4% of the total genera. Among them, A. tenuissima LTJ2 and A. alternata LTJ6 had significant anti-tumor activity. Conclusion The endophytic fungi in Cynanchum bungei Decne. have rich diversity. Some strains have significant anti-tumor activity，which can be potential resources for the development of new antitumor agents.

Research Progress of Baishouwu in the Last Decade / 中国中医药信息杂志

Baishouwu is a traditional tonic Chinese medicinal herb which is rich in Shandong, Liaoning and Jiangsu Privences. Baishouwu mainly contains steroids, polysaccharose, acetophenone, and essential oil, with the efficacy of nourishing liver and kidney, tonifying essence and blood, blackening the beard and hair, and prolonging life. This article retrieved relevant literature in the last decade about biological study, chemical constituents, and pharmacological activities of Baishouwu, with a purpose to provide a scientific basis for its rational researches and development.

Sequence analysis of rDNA-ITS of Bai Shouwu from different species / 中国中药杂志

<p><b>OBJECTIVE</b>To study the genetic diversity of rDNA ITS sequences in different species of Bai Shouwu, utilize the molecular diversity of ITS sequences to authenticate the different species of Bai Shouwu.</p><p><b>METHOD</b>Firstly, total DNA was extracted from the different species of Bai Shouwu. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and sequenced after cloning and purification.</p><p><b>RESULT</b>From four species the complete sequence of ITS and 5.8 S rDNA, the partial sequences of 18S rDNA and 26S rDNA were obtained. The rDNA ITS sequences of Cynanchum bungei (sign in No. GU198970 and No. GU479037) were obtained. Ten variable sites among the sequences were found.</p><p><b>CONCLUSION</b>ITS sequence could be used to authenticate the species. The method could be used to identify germplasm resources and authenticate.</p>

Chemical constituents of Bidens bipinnata / 中草药

Objective To study the chemical constituents of Bidens bipinnata and search for bioactive natural products, which have the anticancer and antiinflammation activities. Methods The six compounds were isolated by a combination of silica gel, reversed phase silica gel column chromatography, and polyamide column chromatography. Their structures were identified by spectral methods. Results Ten compounds were isolated and six structures were identified. They are trideca-2-?-D-glucopyranosyl-1, 13-dihydroxy-3 (E), 11 (E)-en-5, 7, 9-triyne (Ⅰ), trideca -2-?-D-glucopyranosyl-1, 13-dihydroxy-11 (E)-dien-3, 5, 7, 9-tetrayne (Ⅱ), hyperoside (Ⅲ), 6-O-(3″, 6″-diacetyl-?-D-glucopyranosyl)-6, 7, 3′, 4′-(tetrahydroxyaurone) (Ⅳ), 6-O-(6″-acetyl-?-D-glucopyranosyl)-6, 7, 3′, 4′-tetrahydroxyaurone (Ⅴ), 6-O-(4″, 6″-diacetyl-?-D-glucopyranosyl)-6, 7, 3′, 4′-tetrahydroxyaurone (Ⅵ). Conclusion Compound Ⅱ is a novel compound, compound Ⅴ is isolated from this plant for the first time.