RESUMEN
Objective @#To explore the effect of liver-specific knockout of transcription factor EB (Tfeb) on high-fat diet ( HFD ) -induced hepatic steatosis in mice．@*Methods @#Wild-type C57BL /6J mice and liver-specific Tfeb knockout C57BL /6J mice were fed with HFD or normal chow for 12 weeks，respectively，and then the serum tri- glyceride (TG) ，total cholesterol ( TC) ，monocyte chemoattractant protein-1 ( MCP-1 ) ，tumor necrosis factor-α (TNF-α) ，aspartate aminotransferase ( AST) and alanine transaminase ( ALT) were measured in each group of mice ; Western blot was used to detect Tfeb protein expression levels in liver tissues of mice in each group，HE stai- ning was used to monitor histopathological changes in liver tissues of mice in each group，oil red O staining was used to monitor lipid deposition in liver tissues of mice in each group，and F4 /80 fluorescence staining was used to monitor macrophage infiltration in liver tissues of mice in each group.@*Results @#There was no expression of Tfeb gene in liver of liver-specific knockout Tfeb mice，suggesting that the effect of Tfeb gene knockout in liver tissue was better．Compared with the normal control group，the expression of Tfeb protein in the liver tissue of the model control group was down-regulated．Compared with the normal control group，both the HE staining results and the oil red O staining results showed that the liver specific Tfeb caused lipid deposition and liver lobule disorder in mice， which was similar to the liver changes in the model control mice，however，liver-specific knockout of Tfeb mice at 12 weeks of HFD had more severe liver lipid deposition and hepatic lobular structural disorder．The results of F4 / 80 fluorescence staining indicated that the specific knockout of liver Tfeb could aggravate the infiltration of macro- phages in the liver of mice induced by high-fat feeding．At the same time，the serological test results indicated that compared with the normal control group，the serum levels of TC，TG，TNF-α , MCP-1，AST and ALT in the liver- specific knockout Tfeb group and the model control group increased ，and these changes were further elevated in Tfeb knockout mice after HFD feeding．@*Conclusion @#Liver-specific knockout of Tfeb aggravates HFD-induced he- patic steatosis in mice.
RESUMEN
OBJECTIVE:To explore the optimal steaming time of Carapax trionycis during cleansing period,and to optimize and improve production technology of Carapax trionycis recorded by current Chinese Pharmacopoeia. METHODS:The mechanical processing replaced the artificial processing method in Chinese Pharmacopoeia. The content of protein and the appearance of Cara-pax trionycis were investigated after steaming for 30,60,90,120,180,240 min during cleansing period. The extract,decoction, ash content,appearance and property of Carapax trionycis decoction piece processed with vinegar were also investigated after cleansed Carapax trionycis decoction piece was processed by sand scalding and vinegar quenching method. RESULTS:The differ-ent steaming time obtained different quality of cleansed Carapax trionycis decoction piece and Carapax trionycis decoction piece pro-cessed with vinegar. Compared with decoction piece steamed for other duration,when the steaming time was 90 min,the content of protein in cleansed Carapax trionycis decoction piece was higher(31.16%),and its appearance was up to the requirement. Cara-pax trionycis decoction piece processed with vinegar had higher contents of extract and decoction(9.13%,11.39%)and lower con-tent of ash(66.29%),and its appearance was up to the requirement. CONCLUSIONS:Different steaming time have certain effect on the quality of cleansed Carapax trionycis and Carapax trionycis processed with vinegar,the optimal steaming time of Carapax tri-onycis is about 90 min during cleansing. The mechanical processing method maybe replace the artificial processing on Carapax tri-onycis for improving its production efficiency.