RESUMEN
Monocytes,Kupffer cells,spleen macrophages,lung and peritoneal macrophageswere isolated from same rat simultaneously.The effector cells from diverse anato-mical sites were checked for their tumor cytostatic properties using an in vitro tar-get cell growth inhibition assay through measuring (~3H)-TdR incorporation intotumor cell DNA.All of them expressed spontaneous cytostatic activity against hu- man SMMC-7721 hepatoma and mouse YAC-1 lymphoma cells.The effector cells,except monocytes,were shown to exert inhibition on various target cells with diffe-rences in their susceptibility.The cytostasis against either tumor cell lines variedwith five types of effector cells.The most effective cells for inhibiting the growthof lymphoma were monocytes,and Kupffer cells for heptoma,The effector cellsstimulated by Corynebacterium parvum (CP) in vitro were shown that their cytostaticactivity against YAC-1 was increased up to 140-408%,but to heptoma,it wasdecreased to 48-64%.Furthermore,the efficiency of CP stimulation varied withdifferent effector cells also.It is concluded that monocytes and macrophages withsuch natural selective tumor cytostatic capacity were thus everywhere readily availa-ble for activation.CP may enhance or inhibit the cytostatic effect of monocytes andmacrophages on diverse tumor cell lines.It might suggest that the use of CP in thetumor immunotherapy should be considered with different type of tumors.
RESUMEN
Thirty-six male adult rats were divided into 12 groups. The rats, except the control group, were partially (68％) hepatectomized and then killed at intervals between 12-120h after operation. Isolated hepatocytes were prepared and flow cytometry was used to study the proliferative cycle. Mitotic index and binuclear cell count have been performed in liver sections and smears of isolated hepatocytes separately. Tetraploid cells acounted for 76% of all hepatocytes in normal rats and they also constituted the main proliferative population in regenerating liver. During 12-20h after operation, some of the tetraploid cells that remained in G_2 phase entered into mitosis. At 24h after operation, peaks of S phase in tetraploid cells and of octoploid cells occurred. At 36h after operation, mitotic index reached a maxium value and thereafter the percentage of binuclear cells were reduced rapidly. At 48-72h after operation, second peak of DNA synthesis occurred, but showed wide individual variations in time and cell proportion.
RESUMEN
Male Wistar rats were performed 70% partial hepatectomy (PH) or 1.5mg/g body weight D-galactosamine injection (Gal) intraperitoneally. From the 1st to the 7th day after treatment, the serum concentration of alpha-fetoprotein (AFP) and albumin (ALB) was measured and the liver sections were stained with routine and immunohistochemical methods and observed with light and electron microscopy. The mitotic index (MI) reached a maximum level (1.5%) on the 2nd day after PH, while the Gal group showed a lower (0.8%) and delayed (on the 3rd day) MI peak. On the 2nd and 3rd day after PH, AFP positive hepatocytes were predominantly observed in the periportal area and ALB positive cells mainly distributed around the central veins. In Gal group, AFP was detected both in hepatocytes near the necrotic areas and particularly in some special small cells which had not been seen in PH group. These small cells seemed to be able to transform into typical hepatocytes. Intracellular localization of AFP and ALB was observed in the perinuclear space, RER, and Golgi complex. Serum AFP and ALB levels in both groups presented reciprocal changes from the 1st to the 7th day after treatment.