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1.
Journal of Practical Stomatology ; (6): 758-762, 2017.
Artículo en Chino | WPRIM | ID: wpr-697420

RESUMEN

Objective:To evaluate the dental biofilm penetration efficiency of a novel stabilized stannous-containing sodium fluoride dentifric(EXP) and its lipopolysaccharide (LPS) neutralization efficiency.Methods:A controlled,randomized,examiner-blind in situ clinical trial was conducted with the treatment of PBS(control),EXP and a marketed stannous-containing sodium fluoride dentifrice (MKD).Fluorescent dye,fluorescent probe and fluorescence colocalization were used for sample examination and analysis.Results:EXP offered better stannous penetration into the biofilm than MKD and PBS(P <0.05),as well as greater LPS neutralization efficiency(P <0.05).There was a 96.52% overlap of stannous ions and bounded LPS at the same sites treated by EXP.Conclusion:EXP is more effective than MKD in the delivery of stannous into the biofilm and in the neutralization of LPS.

2.
Journal of Southern Medical University ; (12): 1014-1018, 2015.
Artículo en Chino | WPRIM | ID: wpr-355240

RESUMEN

<p><b>OBJECTIVE</b>To investigate the role of sphingosine kinase 1 (SphK1) in regulating the proliferation of hypoxia-exposed glioma cells in vitro and explore the possible molecular mechanisms.</p><p><b>METHODS</b>Human glioblastoma U87MG cells was transfected with specific small interfering RNA (siRNA) constructs targeting SphK1, and the efficiency of SphK1 knockdown was validated by real-time PCR and Western blotting. The cells transfected with SphK1 siRNA and with a negative control siRNA were then exposed to 3% oxygen or 150 µmol/L CoCl2 to induce hypoxia. The cell proliferation and cell cycle changes following the exposure were evaluated with the Cell Counting Kit-8 and flow cytometry, respectively, and the intracellular Ca(2+) changes were monitored using Flou-4/AM under an inverted laser scanning confocal microscope.</p><p><b>RESULTS</b>SphK1 knockdown significantly reduced hypoxia-induced calcium reflux and suppressed the cell proliferation. Application of OAG, an activator of calcium channels, however, obviously enhanced the cell proliferation under hypoxia.</p><p><b>CONCLUSION</b>SphK1 promotes the proliferation of glioma cells under hypoxia via regulating calcium signaling.</p>


Asunto(s)
Humanos , Señalización del Calcio , Ciclo Celular , Hipoxia de la Célula , Línea Celular Tumoral , Proliferación Celular , Glioblastoma , Glioma , Patología , Fosfotransferasas (Aceptor de Grupo Alcohol) , Metabolismo , ARN Interferente Pequeño
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