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Objective To investigate the Allelic and haplotype frequency distribution of seventeen short tandem repeat loci of Y chromosome in Heiyi Zhuang ethnic groups in Guangxi province.Methods Seventeen Y-STR loci,of which the template DNAs were extracted from blood samples of 184 unrelated male individuals in Heiyi Zhuang population,were amplified by using the AmpFlSTR YfilerTM.The PCR products were genotyped with ABI PRISM 310 genetic analyzer.Results The Gene diversity ranged from 0.4910 to 0.9727 at DYS456、DYS389Ⅰ、DYS390、DYS389Ⅱ、DYS458、DYS19、DYS385a\b、DYS393、DYS391、DYS439、DYS635、DYS392、Y-GATA-H4、DYS437、DYS438、DYS448.A total of 180 different haplotypes were observed,The haplotype diversity value calculated from all 17 loci was 0.99976.The significant difference of the allelic frequency distribution in Y-STR loci was found between Heiyi Zhuang population and other observed populations.Conclusion The 17th Y-STR loci in Heiyi Zhuang population of Guangxi province are highly affluent genetic polymorphic and can offer valuable genetic datas for paternity testing and paternal genetic lineages evolution.
RESUMEN
Purpose:To investigate the effect of p53 gene transfection and 1,2∶5,6-dianhydro-3,4-diacetylgalactitol (DADAG) on the growth of hepatocellular carcinoma(HCC). Methods:The HCC line HLE that were transfected with plasmid pUHD10-3 recombined with wt-p53 gene by lipofectamine-mediated were cultured in medium containing DADAG. 3-(4,5-Dimethy-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromid(MTT) technique was used to analyse the growth of HCC cells. Results:The growth of HCC cell line HLE were inhibited after being transfected with wt-p53 gene,and 67.68% of the cells were inhibited on the fourth day. 53.09% of HCC cell line HLE were inhibited when they were exposed to DADAG. When the HCC cell line HLE transfected with wt-p53 gene were exposed to DADAG,85.37% of the cells were inhibited. Conclusions:The inhibition efficiency of HCC was enhanced by the combination of DADAG and wt-p53 gene transfection.