RESUMEN
AIM: To establish method of determing baicalin,chlorogenic acid and chlorpheniramine maleate in Cang'ebiyan Tablets(Fructus Xanthii,Herba Cemtipedae,Radix Astragali,Flos Chrysanthemi indici,etc). METHODS: The VP-ODS C_(18) column(4.6 mm?250 mm,5 ?m) was used with a mobile phase of acetonitrile-0.4%H_3PO_4 0-9 min(14∶86),9-20 min(28∶72) gradient elution,the wavelength of detector was set at 0-9 min 327 nm for determining chlorogenic acid content,9-20 min 280 nm for determining baicalin.The VP-ODS C_(18) column(4.6 mm?250 mm,5 ?m) was used with a mobile phase of acetonitrile-0.5% sodium dodecylsulfate-H_3PO_4,the wavelength of detector was set at 262 nm for determining chlorpheniramine maleate. RESULTS: The calibration curves were linear in the range of 0.21-3.14 ?g/mL(r=0.999 9) for baicalin,in the range of(0.05-0.81 ?g/mL)(r=0.999 9) for chlorogenic acid,in the range of 0.10-1.57 ?g/mL(r=0.999 9) for chlorpheniramine maleate,respectively.The average recoveries were 102.44%,RSD=0.57%(n=6);(98.68%,)RSD=0.30%(n=6);99.46%,RSD=0.70%(n=6),respectively. CONCLUSION: The method is simple,realizable and reproducible and can be used effectively for the quality control of Cang'ebiyan Tablets.
RESUMEN
AIM: HPLC-ELSD method was established to investigate the content of oleanolic acid and ursolic acid in Compound Isodon Rernifolia(D.Don) Kudo Tablets. METHODS: The determination of oleanolic acid and ursolic acid was conducted by HPLC-ELSD using a Agilent Eclipse XDB-C_(18)(4.6 mm?250 mm,5 ?m) column;The mobile phase consisted of methanol-0.5% ammonium acetate solution(82∶18).The flow rate was 1.0 mL/min.The column temperature was set at 30 ?C.The drift tube temperature was set at 85 ?C.The gas flow rate was 2.0 L/min. RESULTS: The linear ranges of oleanolic acid and ursolic acid were 0.45-2.69 ?g(r=0.999 9) and 0.63-3.75 ?g(r=0.999 3),respectively.The average recoveries of oleanolic acid and ursolic acid were(100.3%)(RSD=1.32%) and 100.6%(RSD=1.66%),respectively. CONCLUSION: The method is simple,accurate,and specific which is better than the original one.
RESUMEN
AIM: To establish the quality standard for Lixieling Pill(Herba Andrographis; Radix Sophorae flavescentis etc.). METHODS: Herba Andrographis and Radix Sophorae flavescentis in Lixieling Pill were identified by TLC. Andrographolide, dehydroandrographolide, matrine and oxgmatrine of pill were determined by HPLC together. RESULTS: The characteristic identification by TLC was distinct and highly specific. The quantitative evaluation of andrographolide had the linear range of 0.969 -2.422 ?g, The average recovery was 98.45% and RSD was 0.88. The quantitative evaluation of dehydroandrographolide had the linear range of 0.481 -1.204 ?g , The average recovery was 100.53% and RSD was 0.63. The quantitative evaluation of matrine had the linear range of 1.032 -2.064 ?g , The average recovery was 99.06% and RSD was 1.04. The quantitative evaluation of oxgmatrine had the linear range of 1.016 -2.032 ?g , The average recovery was 99.67% and RSD was 1.20. CONCLUSION: The method is reliable, accurate and specific. It can be used for quality control of Lixieling Pill.