RESUMEN
Objective:To analyze the serum anti-Müllerian hormone (AMH) levels in women of childbearing age in different age groups in Henan, and establish the medical reference intervals based on measurement results from this population.Methods:From January to June 2017, 620 healthy women of childbearing age (20-34 years old), who underwent pre-pregnancy eugenics and pre-marital checkups in 13 project sites in Henan, were included in this study. Participants were divided into 3 age groups: 20-24 years group ( n=210), 25-29 years group ( n=207), and 30-34 years group ( n=203). Spearman correlation coefficient was used to evaluate the correlation between serum AMH level and age; Kruskal-Wallis H test was used to compare the serum AMH levels of different age groups; Wilcoxon test was used for comparison between pairs; the percentile method ( P2.5, P97.5) was used to establish medical reference interval of serum AMH in women of childbearing age for the whole population and different age groups, respectively. Results:The correlation coefficient between serum AMH and age in women of childbearing age (20-34 years old) is -0.17 ( P<0.001). There was a statistically significant difference in the overall frequency distribution of serum AMH levels among the three different age groups ( H=21.978, P<0.05). Among them, there is a statistically significant difference between the 20-24 years group and the 30-34 years group ( Z=4.292, P<0.05). There is a statistically significant difference between the 25-29 years group and the 30-34 years group ( Z=3.803, P<0.05). The reference range of serum AMH is 0.281-9.693 μg/L in this cohort; the reference range of serum AMH is 0.524-10.760, 0.229-9.200, 0.115-8.200 μg/L for women of childbearing age at 20-24, 25-29 and 30-34 years, respectively. Conclusion:The serum AMH level of women of childbearing age (20-34 years old) decreases with age. It is of great significance to establish the serum AMH reference interval for women of childbearing age in different age groups in Henan.
RESUMEN
Objective To observe the effect of 7 kinds of Chinese herbs(Pericarpium Arecae, Folium Sennae, Fructus Tsaoko, Fructus Amomi, Rhizoma Atractylodis Macrocephalae, Fructus Aurantii, Fructus Aurantii I mmaturus), and 2 kinds of active compounds (bornyl acetate, synephrine) of Chinese herbals on gastrointestinal motility of chronic experimental beagle model. Methods Six beagles were used for inducing chronic experimental model. The beagles’antrum, duodenum, jejunum, ileum and colon were implanted with strain gauges to record canine gastrointestinal motility. Gastric fistula was set up for the intake of Chinese medicine decoction, and the external jugular vein catheter was made for intravenous administration. After modeling, the fasting gastrointestinal motility features of the beagles were observed for 120 min every day, and for 7 continuous days. From the 7th day after modeling, fasting gastrointestinal motility before medication was recorded as fundamental control, and when the interdigestive migrating motor complex (MMC) I phase arrived, the gastrointestinal motilities were sequently recorded after treatment with the 7 kinds of herbs(gavage of 200 mL of the decoction of each herb through gastric fistula), normal saline(200 mL), bornyl acetate(active ingredient of Fructus Aurantii and Fructus Aurantii Immaturus, intravenous injection), and synephrine (active ingredient of Fructus Amomi, intravenous injection). All of the animals were treated with only one kind of Chinese herb one day , and the observation of each herb lasted for 2 continuous days. MMC cycle, frequency of contraction, sum of contraction, amplitude of contraction, average of amplitude, and motor index (MI) were observed by strain gauges. Results On postoperative day 1-6, the animals had gastrointestinal hypomotility and no MMC cycle was recorded. On postoperative day 7, the canine antrum, duodenum, jejunum and ileum showed typical MMC cycle while the colon had irregular MMC cycle at fasting interdigestive period. Compared with the gastrointestinal motilities before treatment and those of the normal saline group, the number of contraction waves and MI were significantly increased in the antrum, duodenum, jejunum, ileum and colon after intervention with Pericarpium Arecae and bornyl acetate, were increased in the duodenum, jejunum, ileum and colon after treatment with Folium Sennae and Fructus Tsaoko (P<0.05), and were also enhanced in the antrum and duodenum after administration of Fructus Amomi(P < 0.05). Treatment with Fructus Aurantii induced the decrease of MI in the jejunum, Fructus Aurantii Immaturus decreased MI of the jejunum and colon, and synephrine decreased the number of antrum contraction waves and MI of the antrum and jejunum(P < 0.05). Conclusion The chronic experimental model is effective for the screening of Chinese herbs for improving gastrointestinal motility. Pericarpium Arecae, Folium Sennae, Fructus Tsaoko, Fructus Amomi, and bornyl acetate can increase gastrointestinal motility, while Fructus Aurantii, Fructus Aurantii Immaturus and synephrine can inhibit the gastrointestinal movement, and Rhizoma Atractylodis Macrocephalae has no effect on gastrointestinal motility.