RESUMEN
Sixty-four out of 189 jaundiced patients at San Lazaro Hospital were defined as acute viral hepatitis cases. Of this number, 22 (34.4%) were positive for hepatitis A markers while 26 (40.6%) were positive for hepatitis B markers. Hepatitis D infection accounted for 1.6%, while non-A, non-B hepatitis accounted for 21.9%.
Asunto(s)
Enfermedad Aguda , Adolescente , Adulto , Anciano , Femenino , Hepatitis A/diagnóstico , Anticuerpos Antihepatitis/análisis , Hepatitis B/diagnóstico , Antígenos de Superficie de la Hepatitis B/análisis , Virus de la Hepatitis B/aislamiento & purificación , Hepatitis C/diagnóstico , Hepatitis D/diagnóstico , Virus de la Hepatitis Delta/aislamiento & purificación , Hepatitis Viral Humana/diagnóstico , Hepatovirus/aislamiento & purificación , Humanos , Técnicas Inmunológicas , Ictericia/microbiología , Masculino , Persona de Mediana Edad , FilipinasRESUMEN
A long term study was carried out at San Lazaro Hospital, Manila, Philippines, monitoring the in vitro response of Plasmodium falciparum to chloroquine, amodiaquine, mefloquine, and quinine. The in vitro effective dose giving 50% inhibition of schizogony was: 0.68 X 10(-6) M/liter blood for chloroquine; 0.18 X 10(-6) for amodiaquine; 0.2 X 10(-6) for mefloquine; and 1.12 X 10(-6) for quinine. The percent of isolates determined to be resistant in vitro was 85.2% for chloroquine, and 1.2% for both mefloquine and quinine. These figures were relatively unchanged over the course of 3 years studied. The in vitro resistance rate to amodiaquine increased from 5.1% in 1982 to 22.2% in 1984.
Asunto(s)
Amodiaquina/farmacología , Animales , Antimaláricos/farmacología , Cloroquina/farmacología , Resistencia a Medicamentos , Humanos , Malaria/parasitología , Mefloquina , Filipinas , Plasmodium falciparum/efectos de los fármacos , Quinina/farmacología , Quinolinas/farmacologíaRESUMEN
The dose response of Plasmodium falciparum isolates in the standard in vitro assay for drug resistance was compared using blood specimens which were centrifuged and washed before cultivation. Washing of the cultures increased the success of cultivation by greater than 100%. Eight cultures which grew using both methods gave similar results in the determination of resistance or sensitivity. The ED50 as determined by probit analysis, was approximately 50% higher in parasites which had been washed before cultivation.
Asunto(s)
Animales , Cloroquina/farmacología , Resistencia a Medicamentos , Humanos , Mefloquina , Parasitología/métodos , Plasmodium falciparum/efectos de los fármacos , Quinolinas/farmacología , Manejo de EspecímenesRESUMEN
Human leucocyte antigens (HLA) were used as genetic markers in an attempt to determine possible host genetic susceptibility or resistance to malarial infections. HLA-A and B typing on lymphocytes from 68 confirmed P. falciparum and 77 P. vivax cases was compared with that found in 66 control subjects with no known history of malaria. A significant deviation was observed in the distribution of HLA-B27. This phenotype was absent in the P. falciparum group although found present in the P. vivax group (10%) and the control group (11%). Also, the combination of A9(w24) and B5 was significantly higher among the P. falciparum group than that found in the P. vivax and control groups. These findings require confirmation but do suggest the possibility of genetic susceptibility and that extensive genetic studies might be worth investigating.