METTL3-mediated m6A modification involved in electrical remodeling of atrial cardiomyocytes under high hydrostatic pressure / 中国药理学通报

To investigate the regulation of N6- methyladenosine ( m6A ) modification on L-type calcium channels in atrial myocytes under high hydrostatic pressure, mediated by methyltransferase-like protein 3 ( METTL3 ). Methods C57BL/6J mice were randomly assigned to the control group and the hypertension group ( treated with continuous administration of angiotensin for four weeks ). Masson staining was used to observe the fibrosis of mouse atrial tissue, while dot blot assay and Western blot were used to detect the levels of m6A, METTL3, and Cavi1 2 in the atrial tissue. A high hydrostatic pressure model was constructed using the HL-1 cell line cultured in vitro, and METTL3 was intervened to observe changes in m6A expression levels, METTL3 and Cavi1 2 levels in cells,and action potential duration ( APD ) and L-type calcium current ( I

Transcriptional co-activator p300 participates in atrial fibrosis induced by high hydrostatic pressure by regulating TGF-β/Smad3 signaling pathway / 中国药理学通报

Aim To investigate the role and potential mechanism of transcriptional co-activator p300 in atrial fibrosis caused by high hydrostatic pressure. Methods The left atrial appendage tissues of humans in three groups of sinus rhythm, atrial fibrillation (AF), hypertension and AF were collected. The expressions of p300 protein and TGF-β/Smad3 signaling pathway and fibrotic factors as type I/III collagen Alphal chain (Col-lAl/Col-3Al), matrix metalloproteinase 2/9 (MMP-2/9) were tested by Western blot. Mouse atrial appendage fibroblasts were cultured under hydrostatic pressures of 0, 20 and 40 mmHg. The fibroblasts cultured under 40 mmHg pressure were treated with curcumin and p300 interference RNA. Western blot was used to test changes in the expression of p300 and the above fibrosis indicators. CCK-8 method was used to test changes of cell proliferation. Results The expressions of p300 and TGF-β/Smad3 signaling pathway proteins and fibrotic factors in AF group and hypertension combined with AF group were significantly higher than those in sinus rhythm group (P < 0. 05). 40 mmHg high hydrostatic pressure stimulation in vitro could increase the expression of p300 and fibrotic factors in fibroblasts (P < 0. 0 5) and enhance the proliferation ability (P < 0. 05). Both curcumin and p300 interfering RNA could reverse the increased expression of p300 and fibrotic factors (P < 0. 05) and decrease cell proliferation (P < 0. 05) induced by hydrostatic pressure. Conclusions High hydrostatic pressure can induce atrial fibrosis, which involves the participation of p300 in this process by regulating the TGF-β/Smad3 signaling pathway.

Midterm-clinical Outcomes after Hip Arthroscopy in Middle-aged Patients with Early Osteoarthritis / 대한고관절학회지

PURPOSE: Although the number of hip arthroscopies is rapidly increasing in non-elderly patients, outcomes of this procedure in middle-aged patients are not well documented or clearly understood. The purpose of this study was to evaluate the clinical and radiological outcomes after hip arthroscopy in middle-aged patients with early osteoarthritis.MATERIALS AND METHODS: This retrospective study analyzed 189 patients with early osteoarthritis of various diagnoses aged 40 years or older who underwent hip arthroscopy between January 2010 and December 2015. Clinical (e.g., modified Harris hip score [mHHS], hip outcome score-activities of daily living [HOS-ADL], visual analogue scale [VAS] for pain, range of motion) and radiological (change of Tönnis grade) outcomes were assessed at a minimum of 3-year follow-up.RESULTS: The mean preoperative and final mHHS and HOS-ADL improved from 61.2 and 60.6 to 79.5 and 81.8, respectively, while the VAS pain score decreased from 6.3 to 3.2 (P<0.001). Although the mean range of internal rotation and flexion increased from 14.2 and 100.7° preoperatively to 30.4 and 110.6° at 1-year postoperatively, they decreased slightly to 27.4 and 105.4° at the final follow-up, respectively. Eight cases (4.2%) underwent revision arthroscopic surgery and three cases (1.6%) were converted to total hip arthroplasty.CONCLUSION: Patients with early-stage osteoarthritis of various diagnoses achieved improved clinical outcomes. Therefore, using hip arthroscopy in middle-aged patients with early osteoarthritis, it is possible to achieve good surgical options.

Acute toxicity of four heavy metal ions to Oncomelania hupensis / 中国血吸虫病防治杂志

Objective To assess the acute toxicity of Cu2+, Cd2+, Hg2+ and Pb2+ to Oncomelania hupensis. Methods Cu2+, Cd2+, Hg2+ and Pb2+ solutions were prepared at five concentrations, and 10 snails were exposed to each concentration for 24, 48, 72 h and 96 h. Then, the inhibition of snail activity and snail death was observed, and the half maximal effective concentration (EC50) and median lethal concentrations (LC50) were estimated. Results The 24, 48, 72 h and 96 h EC50 values of Cu2+, Cd2+, Hg2+ and Pb2+ were 0.74, 0.56, 0.46, 0.37 mg/L, 4.79, 3.52, 1.70, 1.26 mg/L, 1.90, 1.49, 0.83, 0.76 mg/L and 21.40, 9.98, 7.90, 5.42 mg/L for snails, respectively. The 96 h LC50 values of Cu2+, Cd2+, Hg2+ and Pb2+ were 0.43, 2.96, 1.12 mg/L and 12.22 mg/L for snails, the safe concentrations were 0.004 3, 0.029 6, 0.011 2, 0.122 2 mg/L, respectively. Conclusion Cu2+ shows a high acute toxicity to snails, and Cd2+ and Hg2+ exhibit a moderate acute toxicity to snails, while Pb2+ is lowly toxic to snails.

Comparative characterization of maxillary expansion and alternate maxillary expansions and constrictions in rats / 华中科技大学学报（医学）（英德文版）

The purpose of this study was to evaluate whether the cranial and circumaxillary sutures react differently to maxillary expansion (ME) and alternate maxillary expansions and constrictions (Alt-MEC) in a rat model. Twenty-two male Sprague-Dawley rats (6 weeks old) were used and divided into three groups. In ME group (n=9), an expander was activated for 5 days. In Alt-MEC group (9 animals), an alternate expansion and constriction protocol (5-day expansion and 5-day constriction for one cycle) was conducted for 2.5 cycles (25 days total). The control group comprised 4 animals with no appliances used, each of two sacrificed on day 5 and day 25 respectively. Midpalatal suture expansion or constriction levels were assessed qualitatively and quantitatively by bite-wing X-rays and cast models. Distances between two central incisors and two maxillary first molars were measured on cast models after each activation. Circumaxillary sutures (midpalatal, maxillopalatine, premaxillary, zygomaticotemporal and frontonasal suture) in each group were characterized histologically. Results showed that midpalatal suture was widened and restored after each expansion and constriction. At the end of activation, the widths between both central incisors and first molars in Alt-MEC group were significantly larger than those in ME group (P<0.05). Histologically, all five circumaxillary sutures studied were widened in multiple zones in Alt-MEC group. However, only midpalatal suture was expanded with cellular fibrous tissue filling in ME group. Significant osteoclast hyperplasia was observed in all circumaxillary sutures after alternate expansions and constrictions, but osteoclast count increase was only observed in midpalatal suture in ME group. These results suggested that cranial and circumaxillary sutures were actively reconstructed after Alt-MEC, while only midpalatal suture had active reaction after ME.

Comparison of endovascular aortic repair and open surgical repair for ruptured abdominal aortic aneurysm / 中国医学科学院学报

<p><b>OBJECTIVE</b>To compare the clinical efficacies of endovascular aortic repair(EVAR)and open surgical repair(OSR)for ruptured abdominal aortic aneurysm(rAAA).</p><p><b>METHODS</b>The clinical data of 28 rAAA patients undergoing emergent treatment between February 2002 and February 2013 in PUMC Hospital were retrospectively reviewed. Among them 13 cases were treated by EVAR and 15 cases by OSR.</p><p><b>RESULTS</b>Before the surgery,the general conditions,comorbidities,and hemodynamics were not significantly different between these two groups(all P>0.05),although the EVAR group had significantly higher mean age than OSR group(P=0.041). In the perioperative period,the EVAR group showed significantly lower 30-day mortality(P=0.044),less blood loss(P=0.005),less blood transfusion(P=0.003),less infusion quantity(P=0.000),shorter length of procedure(P=0.001),and shorter hospital stay(P=0.020). Also,the EVAR group had no severe perioperative complications and showed superior 1-year follow up survival(P<0.05).</p><p><b>CONCLUSIONS</b>EVAR is an effective treatment for rAAA and can improve the clinical outcomes. EVAR may be adopted as the first-line treatment for rAAA,especially for the aged.</p>

Comparative characterization of maxillary expansion and alternate maxillary expansions and constrictions in rats / 华中科技大学学报（医学）（英德文版）

The purpose of this study was to evaluate whether the cranial and circumaxillary sutures react differently to maxillary expansion (ME) and alternate maxillary expansions and constrictions (Alt-MEC) in a rat model. Twenty-two male Sprague-Dawley rats (6 weeks old) were used and divided into three groups. In ME group (n=9), an expander was activated for 5 days. In Alt-MEC group (9 animals), an alternate expansion and constriction protocol (5-day expansion and 5-day constriction for one cycle) was conducted for 2.5 cycles (25 days total). The control group comprised 4 animals with no appliances used, each of two sacrificed on day 5 and day 25 respectively. Midpalatal suture expansion or constriction levels were assessed qualitatively and quantitatively by bite-wing X-rays and cast models. Distances between two central incisors and two maxillary first molars were measured on cast models after each activation. Circumaxillary sutures (midpalatal, maxillopalatine, premaxillary, zygomaticotemporal and frontonasal suture) in each group were characterized histologically. Results showed that midpalatal suture was widened and restored after each expansion and constriction. At the end of activation, the widths between both central incisors and first molars in Alt-MEC group were significantly larger than those in ME group (P<0.05). Histologically, all five circumaxillary sutures studied were widened in multiple zones in Alt-MEC group. However, only midpalatal suture was expanded with cellular fibrous tissue filling in ME group. Significant osteoclast hyperplasia was observed in all circumaxillary sutures after alternate expansions and constrictions, but osteoclast count increase was only observed in midpalatal suture in ME group. These results suggested that cranial and circumaxillary sutures were actively reconstructed after Alt-MEC, while only midpalatal suture had active reaction after ME.

Intrusion of overerupted maxillary molars with miniscrew implant anchorage: a radiographic evaluation / 华中科技大学学报（医学）（英德文版）

The aim of this retrospective study was to quantitatively evaluate the treatment effects of intrusion of overerupted maxillary molars using miniscrew implant anchorage and to investigate the apical root resorption after molar intrusion. The subjects included 30 patients whose average ages were 35.5±9.0 years. All patients had received intrusion treatments for overerupted maxillary molars with miniscrew anchorage. There were 38 maxillary first molars and 26 maxillary second molars to be intruded. Two miniscrews were inserted in the buccal and palatal alveolar bone mesial to the overerupted molar. Force of 100-150 g was applied by the elastic chains between screw head and attachment on each side. Lateral cephalograms and panoramic radiographs taken before and after intrusion were used to evaluate dental changes and root resorption of molars. Only 6 of the 128 miniscrews failed. The first and second molars were significantly intruded by averages of 3.4 mm and 3.1 mm respectively (P<0.001). The average intrusion time was more than 6 months. The crown of the molars mesially tilted by averages of 3.1 degrees and 3.3 degrees (P<0.001) for first and second molars. The amounts of root resorption were 0.2-0.4 mm on average. The intrusion treatment of overerupted molars with miniscrew anchorages could be used as an efficient and reliable method to recover lost restoration space for prosthesis. Radiographically speaking, root resorption of molars was not clinically significant after application of intrusive forces of 200 to 300 g.

Intrusion of overerupted maxillary molars with miniscrew implant anchorage: A radiographic evaluation / 华中科技大学学报（医学）（英德文版）

The aim of this retrospective study was to quantitatively evaluate the treatment effects of intrusion of overerupted maxillary molars using miniscrew implant anchorage and to investigate the apical root resorption after molar intrusion. The subjects included 30 patients whose average ages were 35.5±9.0 years. All patients had received intrusion treatments for overerupted maxillary molars with miniscrew anchorage. There were 38 maxillary first molars and 26 maxillary second molars to be intruded. Two miniscrews were inserted in the buccal and palatal alveolar bone mesial to the overerupted molar. Force of 100-150 g was applied by the elastic chains between screw head and attachment on each side. Lateral cephalograms and panoramic radiographs taken before and after intrusion were used to evaluate dental changes and root resorption of molars. Only 6 of the 128 miniscrews failed. The first and second molars were significantly intruded by averages of 3.4 mm and 3.1 mm respectively (P<0.001). The average intrusion time was more than 6 months. The crown of the molars mesially tilted by averages of 3.1 degrees and 3.3 degrees (P<0.001) for first and second molars. The amounts of root resorption were 0.2-0.4 mm on average. The intrusion treatment of overerupted molars with miniscrew anchorages could be used as an efficient and reliable method to recover lost restoration space for prosthesis. Radiographically speaking, root resorption of molars was not clinically significant after application of intrusive forces of 200 to 300 g.

Malocclusions in Xia Dynasty in China / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The prevalence of malocclusion in modern population is higher than that in the excavated samples from the ancient times. Presently, the prevalence of juvenile malocclusion in the early stage of permanent teeth is as high as 72.92% in China. This study aimed to observe and evaluate the prevalence and severity of malocclusions in a sample of Xia Dynasty in China, and to compare these findings with the modern Chinese population.</p><p><b>METHODS</b>The material consisted of 38 male and 18 female protohistoric skulls of Xia Dynasty 4000 years ago. Of 86 dental arches, 29 cases had the jaw relationships. Tooth crowding, diastema, individual tooth malposition and malocclusion were studied.</p><p><b>RESULTS</b>Of the samples, 23.3% showed tooth alignment problems including crowding (8.1%), diastema (9.3%), and individual tooth malposition (5.8%). The prevalence of malocclusion was 27.6%, mainly presented as Angle Class I.</p><p><b>CONCLUSIONS</b>It is indicated that over thousands of years from Neolithic Age (6000 - 7000 years ago) to Xia Dynasty (4000 years ago), the prevalence of malocclusion did not change significantly. The prevalence of malocclusion of Xia Dynasty samples was much lower than that of modern population.</p>

Changes of blood flow volume in dog's mental artery after mandible osteocompression / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To observe the changes of blood flow volume in mental artery of dog before and after osteocompression.</p><p><b>METHODS</b>16 adult dogs were selected and the osteocompressors were fixed on the right buccal side of the mandible. The osteocompressor was used to compress the mandible 1.0 mm per 3 days for totally 15 days. On the 1st, 15th, 45th and 90th day after osteocompression, the 4 MHz prober of color Doppler flow imaging machine was put on the surface of the mucosa below which there was the mental artery. The color Doppler flow imaging was used to measure the blood flow volume in the mental artery of both experimental and control sides.</p><p><b>RESULTS</b>1) The blood flow value in the mental artery was the lowest on the 15th day after osteocompression, however there was not significantly different before and after osteocompression. 2) Compared with the control, the blood flow value of the mental artery on the 1st day after experiment was significantly decreased. 3) Compared with the data before experiment, the deduction of the blood flow values between experimental side and control on the 1st, 15th and 45th day after osteocompression had significant differences. On the 90th day, the deduction of the blood flow value was similar to normal.</p><p><b>CONCLUSION</b>The osteocompression influences blood flow volume in mental artery temporarily, however, the influence is reversible and can be recovered by times after osteocompression.</p>

Clinical evaluation of preadjusted appliance based on Chinese normal occlusion / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the effect of the preadjusted appliance (Z1 appliance) based on Chinese normal occlusion.</p><p><b>METHODS</b>Thirty non-extraction patients were treated with Z1 appliance. No wire bending was made during treatment. The results of these 30 cases had been evaluated by five senior orthodontists. Models were analysed quantitatively to testify and evaluate the efficiency of the appliance.</p><p><b>RESULTS</b>The average score for post-treatment models was 91.2 (total score was 100). The model measurements indicated that average projective distance on occlusal plane between proximal contact points of adjacent teeth was less than 0.3 mm, which demonstrated good alignment. The prominence of crowns was almost coincident with the data of Chinese normal occlusion. For the average tip of most teeth, there was no statistical difference between the study group and the normal control group. The crown torque variation tendency from anterior to posterior was similar to that in the subjects with normal occlusion. However, the cases treated with Z1 appliance achieved more negative torque in the lower posterior segment.</p><p><b>CONCLUSIONS</b>The result of the non-extraction patients treated with Z1 appliance was quite satisfied. Wire bending could be reduced during treatment. It was necessary to adjust the crown torque values for lower posterior teeth.</p>

Dental caries in ancient Chinese in Xia Dynasty / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the ancient teeth helped us to acquaint with the evolution of oral diseases.</p><p><b>METHODS</b>Teeth and jaws excavated in Erlitou, Henan province and Youyao, Shanxi province were observed clinically with care. The total specimens included 56 individuals (38 males and 18 females, including 894 permanent teeth and 86 dental arches). The dental caries were observed. The incidence of dental caries was calculated.</p><p><b>RESULTS</b>The rate of dental caries was 6.9% in 894 observed teeth. The prevalence was 39% among individuals.</p><p><b>CONCLUSIONS</b>The rate of dental caries in ancient Chinese in Xia Dynasty was lower than that in modem people. There were more caries in elderly people while no sex difference was found in Chinese in Xia Dynasty, which was similar to the situation in modern society. Cervical caries was more common than occlusal caries. The second molars and the third molars were frequently involved, which was different from the phenomenon nowadays.</p>

Correlation of upper airway and surrounding tissues with body mass index in non-apnea aged males / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To approach the correlation of upper airway and surrounding tissues with body mass index (BMI) of non-apnea aged males.</p><p><b>METHODS</b>Thirty-one non-apnea aged from 70 years to 84 years males (all were inspected by polysomnography and apnea hyponea index < 10 times/hour) had magnetic resonance imaging scanned. In images of MRI, the upper airway was divided into four sections, including nasopharynx, velopharynx, glossopharynx and laryn-gopharynx. The sizes, forms and surrounding tissues were measured and correlation analysis of duality vaniable about upper airway and surrounding tissues with BMI were carried out.</p><p><b>RESULTS</b>(1) The volume of nasopharynx had negative correlation with body mass(P < 0.05). (2) The min anterior-posterior diameter of velopharynx had positive correlation with body mass and BMI. The max value of anterior-posterior diameter/transverse diameter of velopharynx had positive correlation with body mass. The mi value of distance of fatpads had negative correlation with body mass and BMI. The min and average value of distance of ascending ramus had positive correlation with body mass and BMI. The mn value of distance of fatpads/distance of ascending ramus had negative correlation with body mass and BMI. The transverse diameter/lateral velopharyngeal wall of velopharynx had positive correlation with BMI. All of above results had statistic significance (P < 0.05). (3)The volume of soft palate, tongue and fatpads had no correlation with body mass and BMI (P > 0.05).</p><p><b>CONCLUSION</b>The forms of sectional areas of velopharynx are subjected to body mass, and with the increasing of body mass they tend to round. In non-apnea aged males, the distance of fatpads and ascending rami have intimate relationship with body mass and BMI. The volume of soft palate, tongue, tatpads and velopharyngeal walls has little things with hody mass and BMI.</p>

Z2 appliance: the pre-adjusted appliance based on Chinese normal occlusion / 华西口腔医学杂志

Z2 appliance is the pre-adjusted appliance designed for Chinese orthodontic patients. The prescription of the appliance is based on Chinese normal occlusion, which is much different from the West in the first and the second orders as well as the third one. The appliance routinely includes 20 brackets and 8 molar buccal tubers with 3 standard arch forms. Clinically, continued light force is used in whole treatment. The side-effects such as forward tipping of incisors, bite deepening and loss of molar anchorage are reduced further due to fewer tips built into the anterior brackets as well as lower friction elastometric modules used during aligning and leveling. In condition of arch are leveled completely, 0.48 mm x 0.64 mm stainless steel archwire with 1.47 N retraction force is the best combination for sliding mechanics, which is proved by 3D nonlinear finite element study. Self drilling micro-screw is used for maximum anchorage. In finishing stage 0.53 mm x 0.64 mm NT arch wire is added in order to get full torque expressing. The research of Chinese pre-adjusted appliance has been lasted for more than 10 years in the department and clinical studies on Z2 appliance show that with minimal wire bending, treatment is more efficient and result is high quality and more consistent for Chinese orthodontic patients.

Magnetic resonance imaging survey of the upper airway in different age non-snoring males / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate the morphology characters of upper airway and its surrounding tissues in different age non-snoring males.</p><p><b>METHODS</b>Total of 114 non-snoring males, with age-range from 22 to 78 year old, were included in this study. They were divided into 3 groups: 30 in younger group (22-29 years old), 57 in middle-aged group (36-57 years old), and 31 in aged group (70-78 years old). All subjects had magnetic resonance imaging scanning.</p><p><b>RESULTS</b>(1) The nasopharynx in aged group was statistically different from that of the younger group and the middle aged group. The volume of nasopharynx in aged group (7.81 +/- 1.59) cm3 (x +/- s, same herein after) is greater than that in younger group (4.89 +/- 1.20) cm3 and middle aged groups (6.06 +/- 2.07) cm3. (2) The AP diameter/transverse diameter of velopharynx, glossopharynx and laryngopharynx in the aged group tended to be smaller. The average value of AP diameter/transverse diameter of velopharynx in aged group is 0.46 +/- 0.14, while the value is 0.59 +/- 0.14 and 0.57 +/- 0.14 in middle and young groups respectively, compared with that of the aged group, there is statistical difference (P < 0.01). Further more, the min/max section area of velopharynx, glossopharynx, laryngopharynx in aged group are smaller than that of young group and midlife groups. (3) The aged group had the thinnest thickness of posterior and lateral velopharyngeal walls, but the largest volume of soft palate and fat pads among all groups.</p><p><b>CONCLUSIONS</b>In non-snoring males, there is more fat deposition around the upper airway with aging. While the large nasopharynx and large transverse diameter of upper airway seem to balance the disadvantaged changes and to benefit the their sleep respiration.</p>

Morphology of upper airway and surrounding tissues in non-apnea males aged seventy years or over: a magnetic resonance imaging study / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To describe the morphology of upper airway in non-apnea males aged seventy years or over.</p><p><b>METHODS</b>Thirty-one non-apnea males aged 70 years or over [diagnosed by whole-night polysomnography (PSG), apnea and hypopnea index (AHI) < 10 per hour] had been taken magnetic resonance imaging (MRI) scanning.</p><p><b>RESULTS</b>The narrowest point of upper airway was at velopharynx. The mean and the minimum section areas were (190.9 +/- 67.1) mm(2) and (112.1 +/- 47.7) mm(2), respectively. Each sagittal/transverse ratio was between 0.22 and 0.89. It showed an ellipse-like cross-section of upper airway, while velopharynx was the most "slender" segment (sagittal/transverse-caliber was 0.22 - 0.71). The min/max section area of upper airway was between 0.38 and 0.62. It demonstrated that each airway segment was a "cone-like" form, while the velopharynx was the steepest segment.</p><p><b>CONCLUSIONS</b>In the non-apnea elderly males aged seventy years or over, the velopharynx was most likely to collapse.</p>

Magnetic resonance imaging in the assessment of the temporomandibular joint structure following mandibular advancement / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the changes in the disc-condyle-fossa relationship following mandibular advancement.</p><p><b>METHODS</b>Twenty-five patients (10 boys and 15 girls, 11.1 +/- 1.1 years) with class II division 1 malocclusions were treated with Activator. The average treatment time was 10.9 months. Parasagittal MRIs in closed mouth position were analyzed before and after Activator treatment by means of the pair-t test.</p><p><b>RESULTS</b>Ca-Tm value [(-0.1 +/- 1.5) mm and (-0.6 +/- 0.9) mm] of the left temporomandibular joint (TMJ) and Ca-Ca' value [(2.3 +/- 0.8) mm and (1.8 +/- 0.5) mm] of the right TMJ were changed significantly after Activator treatment (P < 0.05). This indicated that the condyle was located anteriorly in the fossa. No significant changes were found in the disc-fossa relationship and disc-condyle relationship (P > 0.05).</p><p><b>CONCLUSIONS</b>Activator could promote the growth of the mandible and at the same time, it had no apparent negative influence on TMJ function.</p>

Histological changes of the inferior alveolar nerve after osteocompression on dog's mandible / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To observe the morphologic changes of inferior alveolar nerve before and after osteocompression on dog's mandible.</p><p><b>METHODS</b>8 dogs were selected and divided into 4 groups, 2 dogs per group. The osteocompressor was fixed on the right buccal side of the mandibule. Crew the compressor to compress the mandible 1.0 mm per 3 days for totally 15 days. On the 1st day, 15th day, 45th day and 90th day after osteocompression, the dogs of different groups were killed respectively. Get the inferior alveolar nerve and HE staining was carried on.</p><p><b>RESULTS</b>(1) On the 1st, 15th and 45th day after osteocompression, the fiber of the inferior alveolar nerve was obviously injured. (2) On the 90th day after osteocompression, the injured nerve was almost recovered to normal.</p><p><b>CONCLUSION</b>The osteocompression could cause temporary injury on the inferior alveolar nerve, but the injury was reversible and might recovere by times after osteocompression.</p>

In vitro expansion of cord blood CD133+ cells supported by bone marrow stromal cells and cytokines / 中国实验血液学杂志

The aim of this study was to investigate the effects of human fetal bone marrow stromal cells (FBMSC) in combination with exogenous cytokines on supporting in vitro expansion of CD133(+) cells in cord blood mononuclear cells (MNC). MNCs separated from cord blood (CB) were cultured for up to 14 days in a serum-free system with FBMSC or exogenous cytokines or both of them. On day 0, 6, 10 and 14, total nucleated cells (TNC) were counted; CD133(+) cells were quantified by FACS, and hematopoietic progenitor cells were assessed by semisolid culture assay. The results showed that the number of TNC was remarkably increased in FBMSC and cytokine group, the expansion of CD133(+) cells and CFU were increased in FBMSC and cytokine group except that on day 14. It is concluded that FBMSC play an important role in delaying the differentiation of hematopoietic cells. FBMSC in combination with exogenous cytokines can promote the effective expansion of CB MNC and CD133(+) cells, this expanding system may meet the needs for clinical application of expanded CD133(+) cells.