RESUMEN
The present investigation was carriied out in scald-shocked cats to observe the cardiovascular effects of diphenhydramine ( 2 mg/kg iv ) and cimetidine ( 20mg/kg iv ) . Changes in B-glucuronidase ( B-G ) activity and blood lactate level were also observed.lt was found that inanimals treated with cimetidine the cardiovascular parameter ( BP, LVSP , dP/dtmax, IP, VPm, HR ) were decreased significantly in 2 - 4 h after scalding. These results were the same as those of the saline-treated control. In animals treated with diphenhydramine, the cardiovascular parameters were increased in 1 h after administration of the drug and still persisted at normal level in 4h after scalding.There were no significant differences between groups in changes of B-G activity and the blood lactate level. These data suggest that the benefit of cimetidine in burn-shock is dubious and diphenhydramine may be of value as an adjuvant used in combination with other vasoactive drugs for the treatment of burn shock.
RESUMEN
Objective:To study the effects of ?-GABA on the release of the soluble amyloid precursor protein(sAPP).Methods:Male Sprague-Dawley rats were decapitated and hippocampus was isolated at4℃,then hippocampal slices were quickly prepared in the medium of Krebs-Ringer buffer at0-4℃by a vibration chopper.After the slices were preincubated in Krebs-Ringer buffer at37℃,they were stimulated with various concentrations of ?-GABA(1.0?10 -9 -1.0?10 3 ?mol/L)in Krebs-Ringer buffer.The supernatant was removed from the solution after centrifugated at15000?g.Quantities of total pro-tein were measured by bicinchoninic acid(BCA)assay.The sAPP released into the incubation medium were assayed by West-ern blot.Results:?-GABA increased sAPP release in the incubated hippocampal brain slices.However,it failed to produce special protein.Conclusion:?-GABA within certain concentration range has inhibitory effects on the sAPP release.[
RESUMEN
Our previous work showed that, in the rats with reserpine-induced gastric ulcer, brain monoamines (NE, DA and 5-HT) were exhausted and furazolidone completely prevented such a gastric ulcer.The present study was carried out to observe brain monoamine contents and antiulcerogenic effects of furazolidone in other gastric ulcer modtls of rats.It was found that in the rats with gastric ulcer induced by restraint in water (21℃ for 18[h), brain NE content was significantly decreased.Furazolidone partially prevented this gastric ulcer and increased brain monoamines in these animals.In the rats with gastric ulcer induced by indomethacin or pyloric ligature, brain monoamines were unchanged and no effects of furazolidone were observed.These results suggest that the inhibitory effect of furazolidone on brain monoamine oxidase may be the mechanism of its antiulcerogenic action.