RESUMEN
Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.
Asunto(s)
Animales , Ratas , Genoma Bacteriano/genética , Leptospira/clasificación , Especificidad de la EspecieRESUMEN
Leptospira kirschneri is one of the pathogenic species of the Leptospira genus. Human and animal infection from L. kirschneri gained further attention over the last few decades. Here we present the isolation and characterisation of Brazilian L. kirschneri serogroup Pomona serovar Mozdok strain M36/05 and the comparative genomic analysis with Brazilian human strain 61H. The M36/05 strain caused pulmonary hemorrhagic lesions in the hamster model, showing high virulence. The studied genomes presented high symmetrical identity and the in silico multilocus sequence typing analysis resulted in a new allelic profile (ST101) that so far has only been associated with the Brazilian L. kirschneri serogroup Pomona serovar Mozdok strains. Considering the environmental conditions and high genomic similarity observed between strains, we suggest the existence of a Brazilian L. kirschneri serogroup Pomona serovar Mozdok lineage that could represent a high public health risk; further studies are necessary to confirm the lineage significance and distribution.
Asunto(s)
Animales , Ratas , ADN Bacteriano/genética , Genoma Bacteriano/genética , Leptospira/genética , Cricetinae , Leptospira/patogenicidad , Tipificación de Secuencias Multilocus , Serogrupo , SerotipificaciónRESUMEN
A lipoperoxidacao tem sido associada a diversos mecanismos celulares deleterios que podem acarretar cancer, inflamacao e envelhecimento. Diversas evidencias indicam que os aldeidos, produzidos durante este processo sao mais toxicos do que os monohidroperoxidos dos quais sao originados. Aqui apresentamos um sumario dos principais resultados obtidos com relacao a danos em DNA produzidos por aldeidos reativos. Apesar dos mecanismos de envelhecimento e formacao de tumores ainda serem obscuros, a literatura recente indica a participacao de produtos secundarios da lipoperoxidacao na lesao cromossomal associada a estes processos