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Objective To establish a method for simultaneous determination of icariin and naringin content in bushen huayu extract by HPLC. Methods Reverse-phase high performance liquid chromatography ( RP-HPLC ) separation was performed one C18 column (4.6 mmí250 mm,5 μm).The mobile phase was acetonitrile containing 0.2%H4PO3(pH=2.9). Gradient elution with a flow rate of 0. 8 mL·min-1 was applied to achieve the separation. The detection wavelength was set at 269 nm,and the column temperature was 30℃. Results Icariin had a good linear range from 0. 3-3. 0μg (r=0. 999 9),the recovery rate was 105. 6%,and RSD was 0. 95%. Naringin was linear within the range of 0. 12-1. 20 μg (r=0. 999 9). The recovery rate was 94. 0%and RSD was 0. 52%. Conclusion The method is simple,stable,accurate,and reproducible,which can be used as the quality control standard for bushen huayue extract.
RESUMEN
<p><b>OBJECTIVE</b>To compare the therapeutic effect on sudden deafness between acupuncture and moxibustion therapy of excitation-focus transfer and routine medication.</p><p><b>METHODS</b>Eighty cases of sudden deafness were randomly divided into two groups, 40 cases in each one. Acupuncture and moxibustion therapy of excitation-focus transfer was adopted in observation group on Yongquan (KI 1) (with reducing and slightly heavy manipulation), Tinggong (SI 19), Tinghui (GB 2) and Ermen (TE 21), and associated with suspending moxibustion for thermal sensitization on Yongquan (KI 1). In control group, the routine medications were given. The therapeutic effects of two groups were compared with each other.</p><p><b>RESULTS</b>After three sessions of treatment, dB value of hearing loss in two groups decreased (P<0.05, P<0.01), but the improvement of hearing in observation group was superior to that in control group (P<0.05). The total effective rate was 80.0% (32/40) in observation group, which was better than that 55.0% (22/40) in control group (P<0.05).</p><p><b>CONCLUSION</b>Acupuncture and moxibustion therapy of excitation-focus transfer presents superior therapeutic effect on sudden deafness as compared with the routine western therapy.</p>