Bio-Oss[registered sign] acts on stem cells derived from peripheral blood

This study aims to study how Bio-Oss[registered sign] can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells markers using real time Reverse Transcription-Polymerase Chain Reaction. PB-hMSCs stem preparations were obtained for gradient centrifugation from peripheral blood of healthy anonymous volunteers, using the Acuspin System-Histopaque 1077. The samples were then cultured for 7 days for RNA processing, and the expression was quantified using real time PCR. Bio-Oss[registered sign] caused an induction of osteoblast transcriptional factor like RUNX2 and of bone related genes; SPP1 and FOSL1. In contrast, the expression of ENG was significantly decreased in stem cells treated with Bio-Oss[registered sign] with respect of untreated cells, indicating the differentiation effect of this biomaterial on stem cells. The results obtained can be relevant to enhance the understanding of the molecular mechanism of bone regeneration and can act as a model for comparing other materials with similar clinical effects

Perioglas [R] acts on human stem cells isolated from peripheral blood

PerioGlas [PG] is an alloplastic material used for grafting periodontal osseous defects since 1995. In animal models, it has been proven that PG achieves histologically good repair of surgically created defects. In clinical trials, PG was effective as an adjunct to conventional surgery in the treatment of intrabony defects. Because the molecular events due to PG that are able to alter osteoblast activity to promote bone formation are poorly understood, we investigated the expression of osteoblastic related genes in mesenchymal stem cells exposed to PG. The expression levels of bone related genes like RUNX2, SP7, SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1 and mesenchymal stem cells marker [CD 105] were analyzed, using real time reverse transcription-polymerase chain reaction. Pearson's chi-square [chi[2]] test was used to detect markers with significant differences in gene expression. PG caused induction of osteoblast transcriptional factor [like RUNX2], bone related genes osteopontin [SPP1], osteocalcin [BGLAP] and alkaline phosphatase [ALPL]. All had statistical significant P values [< 0.05]. PG has a differentiation effect on mesenchymal stem cells derived from peripheral blood. The obtained results can be relevant to better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects

Comparison between differential expressed miRNA of osteoblast-like cells cultured with P-15 vs. bio-oss

Bio-Oss is composed of anorganic bovine bone whereas P-15 is an analog of the cell binding domain of collagen. Both are widely used in several bone regeneration procedures in oral surgery. How these biomaterials act on osteoblast activity to promote bone formation is poorly understood. We attempted to get more insight using microRNA microarray techniques to investigate the translation regulation in osteoblasts exposed to P- 15 and Bio-Oss. By using miRNA microarrays containing 329 probe designed -from Human miRNA sequence, we identified in osteoblast-like cells line [MG-63] cultured with Bio-Oss[R] [Geistlich, Wolhusen, Switzerland] and P-15 [Ceramed, Lakewood, CO] several miRNA which expression is significantly modified. There were 4 up-regulated miRNA [i.e. let-7c, mir-27a, mir-125a, mir-30c] and 5 down-regulated miRNA [i.e. mir-23a, mir-320, let-7b, mir-145, mir-128a]. P-15 acts preferentially on homeobox genes whereas Bio-Oss activates preferentially extravellular matrix componets, cytokines and hormones. P-15 and Bio-Oss enhance the translation process of different BMPs: BMP1 and BMP4 the first material and BMP 3 and 11 the second one