RESUMEN
Objective To study the role of lipid metabolic disorders of liver in the formation of calcium bilirubinate gallstone caused by biliary partial obstruction and infection in rabbits. Methods Forty-nine rabbits were divided randomly into 3 groups: the gallstone group A (n=15) and B (n=22), and the control group (group C, n=12). The calcium bilirubinate gallstone model was set up by using partial biliary obstruction and infection in rabbits. Following items were measured:(1)Concentration of plasma triglyceride (TG), phospholipids (PL), total cholesterol (TCh), free cholesterol (FCh), cholesterol with high desitity lipoprotein and its sub-components (HDL-Ch, HDL 2-Ch and HDL 3-Ch); (2)Concentration of bile glycocholic acid (GCA), glycodeoxycholic acid (GDCA) and FCh. Results (1) Compared with group C, the plasma PL increased sighificantly in both group A (P0.05). The plasma HDL-Ch in group B and HDL 2-Ch in group A and B were significantly lower than those in group C (P
RESUMEN
Objective To induce islet grafting tolerance by intravenous injection of anti-CD4,anti-CD8 immunotoxins and donor soluble antigen.Methods 14 days or 7 days prior to transplantation,the immunotoxon 200 μg respectively,and donor soluble antigen 500 μg were injected intravenously into the recipients, then 500 donor islets were translanted under the left renal subcapsular space of diabetes reciPients (SD rats).Results The islet grafting survival time that pretreated with immunotoxon and dono soluble antigen was over 60 days(P<0.01).The immunotoxins or donor soluble antigen treatment alone only slightly prolonged the graft survival.Conclusion The anti-CD4,anti-CD8 immunotoxins combined with donor soluble antigen can induce donor specific immune tolerance.