RESUMEN
The primary aim of our study was to delve into the neuroprotective potential of an ethanolic extract sourced from Alternanthera sessilis, particularly in alleviating behavioural impairments induced by reserpine in rat models simulating stroke and seizures. Our investigation involved categorizing rats into five distinct groups, each consisting of six individuals, with separate sets allocated for different experimental interventions. Seizures and stroke-like symptoms were induced by administering reserpine, after which the rats underwent treatment with varying doses of Alternanthera sessilis ethanolic extract. We conducted a comprehensive assessment of various behavioural parameters, including antiepileptic activity, motor function, overall well-being, rota rod performance, closed field activity, and grip strength. Significantly, our results uncovered substantial improvements in antiepileptic activity, motor function, and overall health of the intervention group, which received the extract, to the control group after the administration period. Additionally, enhancements were noted in rota rod performance, closed field activity, and grip strength, indicative of a marked enhancement in neuroprotective effects. These encouraging findings highlight the potential therapeutic efficacy of Alternanthera sessilis extract in managing stroke and seizure disorders. Nevertheless, further extensive investigations are imperative to unravel the precise mechanisms underlying these observed enhancements. Furthermore, future research endeavors should focus on delineating the specific therapeutic applications of Alternanthera sessilis extract, thus facilitating its potential integration into clinical practice for the management of stroke and seizure-related conditions. This would pave the way for a more comprehensive understanding of its therapeutic benefits and broaden its scope for clinical utility.
RESUMEN
The present study evaluated the potential of an ethanol extract from Alternanthera sessilis to alleviate reserpine-induced Parkinson's disease (PD) like behavioural manifestations in rats. The animals were grouped into five: a control group, a group receiving only reserpine, a group treated with levodopa and carbidopa, and two groups given different doses of the Alternanthera sessilis extract. Reserpine was used to mimic PD symptoms, while the extract was administered orally at two different concentrations. The behavioral tests were conducted to assess locomotor activity, grip strength, catalepsy, and rearing activity. Locomotor activity was measured using an actophotometer, where interruptions in light beams caused by the rates were recorded over a 10-minute period. Grip strength was evaluated using a rotarod apparatus, measuring the time taken for rats to fall off individual rods. Catalepsy was assessed through a bar test, measuring the time for rats to descend from a horizontal bar to the base. Rearing activity was observed in a round open field arena, with parameters including the occurrence rate of rearing, general movements score, and duration spent in the center of the arena. The results revealed administration of reserpine led to a decrease in body weight, locomotor activity, muscle coordination, catalepsy score, rearing activity, and grip strength compared to the control group. Conversely, treatment with levodopa and carbidopa resulted in increased values for these parameters. Importantly, administration of Alternanthera sessilis extract showed a dose-dependent reversal of the effects induced by reserpine, with significant increases observed in body weight, locomotor activity, muscle coordination, rearing activity, general movement, and grip strength, indicating its potential therapeutic effects. In conclusion, the present study results suggest that the extract from the Alternanthera sessilis plant might protect brain cells and improve symptoms of Parkinson's disease. However, more research is needed to understand how it works and if it can be used safely and effectively clinical trails.