RESUMEN
Background: Rumex turcomanicus Czerep. Locally named Sagh-Torshak, is one of native green vegetable medicinally valuable plant belongs to family Polygonaceae, growing spontaneously in Northeast Iran, where its leaves are highly appreciated and consumed. Despite the high consumption, its knowledge is inconsiderable
Objective: The aim of this study was to evaluate the changes of main phytochemical compound in leaf and root parts of R. turcomanicus Czerep. in different phenological stages
Methods: The wild-growing plants were harvested at vegetative, floral budding, full floweringand mature seed stages and dissected into root and shoot tissues, which were dried separately and subsequently assayed for total phenolics, flavonoid, ascorbic acid, oxalic acid content and antioxidant activity, by spectrophotometric method
Results: The results showed considerable contents of phenol, flavonoid and antioxidant activity in roots and shoots of plant, with the higher contents in roots as compared to shoots. Among different phonological stages the highest total phenol, flavonoid, oxalic acid content and antioxidant activity of shoots was obtained in mature seed stage. Ascorbic acid of shoot was highest in floral budding stage. The greatest total phenol and flavonoid content of root was observed in floral budding stage, while the highest antioxidant activity was obtained in mature seed stage. A high negatively correlation was obtained between ascorbic acid and Oxalic acid
Conclusion: The concentrations of secondary metabolites in Rumex turcomanicus Czerep. depend on the phenological stage and tissue of the plant. As plant aged, oxalic acid and ascorbic acid of shoots were increased and decreased, respectively
RESUMEN
Domestic cultivation under natural habitat may help to genetic diversity conservation and sustainable utilization of medicinal plant. Since alkaloids are nitrogenous compounds, the availability of nitrogen is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. The aim of this research was to study the effect of bio-fertilizer and chemical fertilizer on corm and colchicine yield in Colchicum kotschyi under natural habitat. The experiment was performed in a randomized complete block design with eight treatments including of three levels of urea as nitrogen fertilizer [50, 100 and 150 kg ha[-1]], three levels of Nitroxin [20, 40 and 60 L ha[-1]], combined treatment [urea 100 kg ha[-1] + Nitroxin 40 L ha[-1]] and no fertilizer as control with three replications at the natural habitat, during 2009 -2010. results showed that the application of Nitroxin 40 L ha[-1]resulted in the highest corm dry yield [101.4 g], while the lowest corm dry yield was obtained in control [42.39 g]. The yield of colchicin was higher in application of urea 100 kg ha[-1] [40.44 mg] or Nitroxin 40 L ha[-1] [37.58] than to other treatments, while the lowest colchicine yield was obtained in control [15.56 mg]. Generally moderate levels of chemical and biological fertilizers [urea 100 kg ha[-1]or Nitroxin 40 L ha[-1]] had the best results on corm and colchicine yield than to other treatments