Antimicrobial activity of extracts and isolates of the leaves of Plumeria alba Linn.

Disc diffusion method was employed to determine the effect of methanol, chloroform and acetone extracts of the dried leaves of Plumeria alba Linn. [Apocyitaceac] against fungi [Candida albicans] and bacteria [Bacillus subtilis, Proteus vulgaris Staphylococcus aureus. Pseudomonas aeruginosa, Streptococcus fecalis, Escherichio coil, Staphylococcus albus and Klebsiella pneumoniae]. The methanol and acetone extracts exhibited a prominent antimicrobial activity. The methanolic and acetone extracts were further fractionated by column chromatography to yield 2 pure isolates. The methanol pure component-2 [MPC[2]-50% Ethyl acetate:Methanol] and Acetone pure component-2 [APC[2]-70% Acetone:Ethyl acetate] have shown significant activity against all organisms used except Klebsiella