Evaluation of salivary albumin in diabetic patients

The clinical symptoms of diabetes mellitus in oral cavity are xerostomia, pathological changes in periodontal tissues and caries. It has been suggested that the determination of salvia's components in diabetic patients may be useful in describing and further understanding the oral findings in this condition. The aim of this study was to detect differences between the salivary albumin in diabetic patients and healthy people. A case-control study was carried out using data from Hamadan researches diabetes center in 2008. We selected 40 patients with type 1 diabetes mellitus aged 9-61 years, 40 patients with type2 aged 39-82 years and 40 healthy controls aged 20-67 years. Dental and oral status was assessed for all subjects. Completely unstimulated saliva samples were collected from diabetic patients and control subjects. Nephelometric method was used to determine salivary albumin concentration. Statistical analysis was done by SPSS. No significant difference in salivary albumin concentration was found between the control group and type 1 diabetic patients but salivary albumin was significantly higher in type 2 diabetic patients in compare with control groups [P=0.017], also no significant difference was found between type 1 and type 2 diabetic patients. In diabetic patients, complain of dry mouth and burning feeling in mouth was significantly higher, as well as, dental examinations showed higher DMFT mean values than the controls. Adults with type 2 diabetes mellitus have higher concentration of salivary albumin that would play a helpful diagnostic role in oral findings of diabetic patients

[Evaluation of serum levels of interleukin-6 and -8 in patients with recurrent aphthous ulcerations]

Recurrent aphthous ulcers [RAU] are one of the most common ulcers in the oral cavity. Several studies have shown conflicting variations for inflammatory cytokines and other biologic markers in recurrent aphthous ulcers. The aim of the present study was to determine the level of IL-8 and IL-6 in the serum of patients with recurrent aphthous ulcers. In this case- control study, the serum levels of IL-8 and IL-6 were measured in patients with recurrent aphthous ulcers [n=40] and in healthy control subjects [n=40]. The cytokines levels were measured in the serum by ELISA. The data were analyzed with SPSS software using T- test. The mean value of the IL-8 and IL-6 in the patient group was 52.24 pg/ml and 9.39 pg/ml which were 10 and 4 times higher than that of the control group, respectively. The differences were statistically significant [p<0.01]. The results of the present study showed that the serum levels of IL-8 and IL-6 were elevated in the patient group, IL-8 level being more considerable

Association between en dothelial selectin [E-selectin] gene polymorphisms and E-selectin level with visceral leishmaniais, in an ARMS-PCR based study

In the visceral leishmaniasis [VL], parasites reside in reticuluendothelial system, mainly in macrophages. Endothelial Selectin [E-selectin] might play an important role in leukocyte-endothelium interactions and inflammatory cell recruitment. The aim of this study was determining E-selectin level and its polymorphism in three groups, patients, seropositive and healthy individuals. Serum soluble E-selectin levels as well as 2 polymorphisms of E-selectin [Ser 128 Arg and Leu 554 Phe] were measured in a cohort of patients with documented VL [n=64], a healthy control group [n=74] and a seropositive for VL but without any symptoms [n=81]. Circulation concentration of E-selectin levels was measured by ELIS. The amplification refractory mutation system [ARMS]-PCR procedure was used for detecting polymorphisms. The mean of E-selectin levels significantly differed between three groups [P<0.026], and were increased in patients in comparison with other groups. Difference was more considerable between two groups of patients and healthy ones [patients 92.8 ng/ml; healthy individuals 71.9 ng/ml]. Polymorphisms were associated with soluble E-selectin levels and altogether explained 14.4%, 7.2%, and 8.7% in patients, seropositive and seronegative healthy individuals, respectively. Distribution of polymorphisms of 128 Ser/Arg and 554 Leu/Phe among three groups was not different significantly; however, there was a considerable arrangement in distribution of Ser 128 Arg polymorphism and 128 Arg allele in healthy group was more than two fold of patients [55% against 20%]. The association between soluble E-selectin levels and visceral leishmaniasis suggests that this molecule might have significant role in the inflammatory process in VL. Moreover, frequency of 128 Arg allele in healthy group was higher than patients