Antimicrobial susceptibility testing of Escherichia coli strains isolated from urinary tract infections to fluoroquinolones and detection of gyrA mutations in resistant strains

Widespread uses of fluoroquinolones have resulted in increasing incidences of resistance against these agents all over the world. The aim of this study was to assess, susceptibility of Escherichia coli strains from patients with Urinary Tract Infection against common fluoroquinolones and detection of mutations in the gyrA gene. Antimicrobial susceptibility testing of 164 E.coli isolates from patients with UTI, was evaluated by disk agar diffusion [DAD] and MIC methods. Polymerase chain reaction of E.coli strains were performed by amplification of Quinolone Resistance Determining Region [QRDR] of gyrA gene. PCR products were tested by Conformational Sensitive Gel Electrophoresis [CSGE] and those with hetrodublexes were selected and examined by DNA sequencing. According to disc agar diffusion, 49.3% were resistant to nalidixic acid, 41.4% to norfloxacin, 44.5% to ofloxacin and 40.2% to ciprofloxacin. By Minimal Inhibitory Concentration [MIC] testing a high-level of resistance [42.1%] to ciprofloxacin was observed. Mutations in codons 83 and 87 in all 81 isolates were positive by CSGE method

Antimicrobial susceptibility testing for escherichia coli strains to fluoroquinolones, in urinary tract infections

Urinary Tract Infections [UTIs] are one of the most common infectious diseases diagnosed all over the world. Meanwhile most episode of UTIs are caused by Escherichia coli [up to 85%] and frequently fluoroquinolones are preferred as initial agents for empiric therapy of UTIs. Widespread use of fluoroquinolones has resulted in an increasing incidence of resistance these agents all over the world. The aim of this study was to assess, susceptibility of Escherichia coli strains from UTI patients against common fluoroquinolones. Antimicrobial susceptibility testing was determined by disk agar diffusion [DAD] and Minimal Inhibitory Concentration methods as described by the National Committee for Clinical Laboratory Standards [NCCLS]. One hundred sixty four clinical isolates of E. coli were collected by urine cultures from patients with UTI. The extent of resistant to nalidixic acid, ofloxacin, norfloxacin and ciprofloxacin, by disk diffusion method was 49.3%, 44.5%, 41.4% and 40.2%, respectively. Resistance to ciprofloxacin by MIC method was 4.9%. This study represents high level resistant of E. coli isolates from UTI patients. It is because of inappropriate and incorrect administration of antimicrobial agents in blind cases. This problem remarks significance of performing antimicrobial susceptibility testing before empiric antibiotic therapy. To overcome this problem use of unnecessary antibiotics therapy should be limited