[ presence of insulin catalytic antibodies in plasma of two type 2 diabetes patients]

Recent studies have reported the role of catalytic antibodies in the pathogenesis pattern of some diseases. Autoimmune reactions playing a role in some type 2 diabetic patients and the p- cell autoimmune markers were found to be present in some of these patients. The presence of such antibodies was assessed in the plasma of patients suffering from type 2diabetes. Antibodies in 3 diabetic patients and 7 non-diabetic control subjects were purified using protein-G sepharose affinity column chromatography and S-300 gel filtration methods; purity of the IgG antibodies was confirmed by SDS-PAGE and in western blot analysis and proteolytic activity of electrophoretically homogenous IgG antibodies was confirmed with zymogram analysis. The purified antibodies were incubated with insulin at 37°C for 6 days and the effect of antibodies on insulin degradation was assessed by Acetic Acid-Urea polyacrylamide gel electrophoresis. Insulin degradation effect was observed only in purified antibodies in the 2 diabetic patients and it was not seen in the 7 control subjects and the remaining diabetic patients. Our data revealed, for the first time, insulin degradation by isolated IgG from 2 diabetic patients. This finding may not only explain the insulin resistance observed in some diabetic patients, but may most likely propose also a new mechanism for occurrence of the disease

Value and accuracy of fine needle aspiration cytolbgy [FNAC] in thediagnosis of breast masses

Breast cancer is the most common malignancy of the women and causes death inmany patients every year. About 70% of breast surgeries and biopsy taking are not necessarybecause they yield benign diagnosis. For diagnosing breast masses accurately and speciallydifferentiating benign from malignant tumors without surgery, Triple Test has been introduced.This project tried to evaluate the diagnostic value and accuracy of fine needle aspiration cytologyof the breast as the most significant element of this test. During a 3 years period, 120 females with the chief complaint of abreast mass being admitted in Mashhad Imam Reza Hospital were subjected to all components ofthe triple test followed by confirmatory open biopsy. In 100 patients all paraclinical procedureswere obtained and the results were available. FNA cytologic results were classified in three groups as benign, malignant andsuspicious for malignancy. Fifty-five patients had benign and 40 had malignant cytology whichwere confirmed by routine histopathology. From 5 patients with suspicious cytology, 2 yieldedmalignancy and 3 benignancy by tissue confirmation. FNA cytology of the breast is a well tolerated, simple, safe, accurate, cost effective,noninvasive, less complicated procedure and operable in office practice for diagnosing breastlesions, with an accuracy of about 100%, specially when it is employed in association withphysical exam and mammography in the triple test.Based on these results we strongly recommend application of this method as an ideal procedurefor breast diagnosis and elimination of open biopsy in our country, which certainly will result inexpenses reduction

Thalidomide inhibits the angiogenic activity of culture supernatants of multiple myeloma cell line / 中华血液学杂志

<p><b>OBJECTIVE</b>To investigate the pro-angiogenic effects of several multiple myeloma (MM) cell line culture supernatants on human bone marrow endothelial cell (HBMEC) proliferation, migration, and capillary formation, and the anti-angiogenic effects of thalidomide.</p><p><b>METHODS</b>HBMEC was cultured in the presence of MM cell lines (IM9, XG1, U266 and MOLP-5) supernatants. Proliferation and migration of HBMEC were determined, capillary-like tubule formation of HBMEC was examined in fibrin and Matrigel. The inhibiting effect of thalidomide was investigated by adding it into myeloma cell line culture supernatants. Vascular endothelial growth factor (VEGF) was measured by ELISA.</p><p><b>RESULTS</b>(1) MM cell lines culture supernatants promoted HBMEC proliferation and migration. (2) In fibrin and Matrigel, capillary-like tubule network formation promoted by the supernatants. (3) All of these effects could be inhibited by thalidomide. (4) This effect was not related to VEGF in the supernatants.</p><p><b>CONCLUSIONS</b>MM cell line promote proliferation, migration and tubule formation by secreting VEGF or other several cytokines. Thalidomide can inhibit these effects.</p>