RESUMEN
Aim of the work: The present study was planned to investigate the role played by Bee venom in improving the morphometric and morphological changes induced by carbimazole in pregnant albino rats and their embryos
Materials and method: A total number of 60 mature virgin female and 30 male Wistar wistar albino rats [for fertilization] were used in this study.Pregnancy was ascertained by vaginal smears. The experimental animals were divided into the following groups: I- [Normal group]: in this group the pregnant rats were injected intraperitonealy by distilled water [The solvent of both carbimazole and bee venom] by dose 1ml/200g.body weight, from day 1 to day 18 of gestation. II-The group of treated animals: this group was divided into 3 subgroups: 1-The carbimazole group: this group was divided into2 subgroups, the pregnant rats were orally injected at a dose 2 and 3 mg /200 g.body weight, daily from day 1 to day 18 of gestation. 2-The Bee venom group: the pregnant rats were intraperitonealy injected with a dose 0.6 mg/200g. body weight on days 2,3,4,7,8,9,10,11,12,15 and 16 of gestation. 3-The treatment group: the pregnant rats were intraperitonealy injected with Bee venom at a dose 0.6 mg/200g. Body weight 1 hour after the intraperitonealy injection by the 2 doses of carbimazole [2 and 3mg /200g. body weight].The days of injection for both treatments [Bee venom and carbimazole] were as mentioned before
Results. The results of the present study showed that carbimazole treatment with its 2 doses induced highly significant increase in the body weights of pregnant rats, highly significant reduction of the uterus weight with shortness of the horns as well as unequal distribution of the embryos between them, increased number of the resorbed fetuses when comparing with the control group, Bee venom injection revealed improvement of these changes .The orally injection of the 2 doses of carbimazole resulted in very highly decrease in fetuses body weights and lengths; however Bee venom induced obvious improvement as compared with the carbimazole effects. Orally injection of carbimazole at the dose 3mg /200g body weight showed increased fetal mortality rate as compared with the control group; however, intraperitonealy injection of Bee venom resulted in improvement in the rate of live fetuses and never of dead ones was observed after Bee venom treatment. The 2 doses of carbimazole induced lots of malformations of embryos such as variations in the size of embryos of the same mother, the embryos exhibited fragile skin, sub dermal blood coagulation beside edema in different regions of the fetal body ,as well as malformations of the regions of the eye resembled in exophthalmos and rostrum region such as cleft lips, beside clubbed fore and hind limbs , kyphosis of the body of the embryos, exencephaly.Bee venom treatment resulted in control of the changes induced by carbimazole injection. The fetuses were resembled to those of the control group
Conclusion: It is clear that bee venom plays an important role in controlling the morphometric and morphological changesin fetuses of albino rats suffering from hypothyroidism induced by carbimazole
RESUMEN
The present work was planned to investigate the role played by Bee Venom in improving the changes induced by carbimazole in pregnant albino rats and their embryos. A total number of 60 mature virgin female and 30 male Wistar wistar albino rats [for fertilization]. Pregnancy was ascertained by vaginal smears. The experimental animals were divided into 2 main groups: I- The experimental control group [normal group]: In this group the pregnant rats were injected intraperitonealy by distilled water [the solvent of both carbimazole and bee venom] by dose 1ml/200g.body weight, from day 1 to day 18 of gestation. II-The group of treated animals: this group was divided into 3 subgroups: 1-The carbimazole group: this group was divided into2 subgroups the pregnant rats were orally injected at a dose 2 and 3mg /200g.body weight, daily from day 1 to day 18 of gestation. 2-The Bee Venom group: The pregnant rats were intraperitonealy injected with a dose 0.6 mg/200g. body weight at days 2,3,4,7,8,9,10,11,12,15 and 16 of gestation. 3-The treatment group: The pregnant rats were intraperitonealy injected with Bee Venom at a dose 0.6 mg/200g. body weight 1 hour after the intraperitonealy injection by the 2 doses of carbimazole [2 and 3mg /200g. body weight].The days of injection for both treatments [Bee Venom and carbimazole] were as mentioned before. Carbimazole injection at the 2 doses revealed highly significant and very highly significant decrease of total serum proteins, serum albumin, serum T3and T4, however carbimazole injection induced very highly increase in serum globulin, serum AST and ALT. Bee Venom treatment showed improvement in all the above mentioned parameters