RESUMEN
Background: Medicinal plants of Genus Ferula are prennial herbs of Apiaceae family which are widely distributed all around the word. Many of Feula species are exclusive and endemic to Iran which has widespread uses in traditional medicine as food additive, as well as a carminative, antispasmodic and expectorants. Coumarins, flavonoids and sulfur-containing compounds with different biological effects have been reported from the plant
Objective: The phytochemical investigation on total extract of the aerial parts of Ferula persica Willd var. Latisecta collected from the Central Alborz Protected Area was subjected with the aim of purification and determination of main metabolite constituents of the plant extract
Methods: Dried and powdered plant material was extracted by maceration method. Then the extract was concentrated under reduced pressure to leave a residue which was defatted and chromatographed on a silica gel column by increasing slightly the polarity with solvent. After the serial fractionation the fractions were compared by TLC, and those giving similar coumarin spots were combined and further purified on PLC to give a pure natural compound
Results: After the purification step the remarkable constituents were obtained. Elucidation of results by spectral methods [IR, 1H-NMR, 13C-NMR and DEPT] demonstrate that a coumarin namely: Auraptene [7-genaryloxycoumarin] was isolated from the aerial parts of F. persica. var. latisecta
Conclusion: F. persica is an endemic and endangered species of medicinal plants of Iran which contains different natural compounds such as coumarins. So the Protection of the plant for its economic and scientific uses is so important
RESUMEN
Background: Nowadays treatment and control of pain is still one of problematic cases and syntetic analgesic drugs have several side effects
Objective: The aim of this study is to investigate the antinociceptive effects and phytochemical analysis of Ferula persica with respect to side effects of chemical drugs
Methods: The extract of plant inflorescence was prepared by percolation method. HPLC was used for phytochemical studies. In order to nociception test, male mice were used in acetic acid, immersion and formalin test. Mice were divided into experimental, control and positive control groups [receiving morphine] in each test
Results: In phytochemical analysis, four flavonoids consist of Quercetin, Luteolin, Apigenin and Rutin were identified. In all 3 pain tests, the experimental group had a significant difference with control group [P<0.05], but there was no significant difference with morphine group [P>0.05]
Conclusion: Methanolic extract of Ferula persica has antinociceptive effects, probably due to its sesquiterpenes and flavonoids