Detection of extended specturm beta lactamase [ESBL] producing organisms in enterobacteriaceae family like escherichia coli [E. coli] and klebsiella pneumoniae by double disc diffusion method [DDDM]

Extended spectrum beta lactamase [ESBL] producing organisms are extremely broad spectrum beta-lactamase enzymes found in a variety of enterobacteriaceae. Most strains producing these beta lactamases are Klebsiella pneumoniae and Escherchia coli [E. coli]. When producing these enzymes, organisms become highly effective at inactivating various beta lactam antibiotics. In addition, ESBL producing bacteria are frequently resistant to many classes of antibiotics, resulting in difficult to treat infections. Other problems due to ESBL producing bacteria are difficulty in detecting the presence of ESBLs limiting treatment options and detoriation impact on clinical outcomes. Hence it is very important that a doctor should be familiar with the clinical significance of these enzymes and potential strategies for dealing with this growing problem