Microorganisms-digestive enzymes interactions

The viability of B. subtilis, E. coli, Ps. aeruginosa and C. albicans in 0.5% sterile solutions of diastase, pancreatin, papain, pepsin and trypsin during a period of 7 days was followed by the viable count technique. Marked growth of these organisms took place, though the extent of which varied according to the enzyme. At 4C, the enzymes suffered practically no alteration in their amylolytic or proteolytic activities. Storage at 25C resulted in significant reductions of amylolytic activity [0.3-89.7%] and proteolytic activity [0-77%], depending on the enzyme-organism system. B. subtilis and C. albicans exerted, in general, the highest inactivation of the enzymes. On the other h and, Ps. aeruginosa produced little effect