Modified laser nephelometric technique to study RBC's aggregation stages

Laser nephelometric technique is developed to study RBC's aggregation. Using this aggregometer, blood aggregation curve is obtained from monitoring the scattering light signal from blood under various shear rates. From this curve, blood aggregation relaxation times [relaxation time of RBC's rouleaux formation T1 and time of network formation T2] and aggregation durability constant [beta] are determined. Results obtained indicate that as the shear rate increases the intensity of backscattered light increases due to increase in disaggregation. The relaxation time of formation of rouleaux [T1] was found to be correlated with HCT [hematocrit], RBC's count and HGB [hemoglobin]. Relaxation time of network formation [T2] and hydrodynamic durability constant [beta] have a weak correlation with hematological parameters

Modified laser viscometer technique to measure blood viscosity

To eliminate human factor errors in measuring the blood viscosity laser viscometer was designed and constructed. Blood viscosity of ten human blood samples were measured using this viscometer. Hematological parameters of these samples [RBC's countt, mean cell volume [MCV] hematocrit [HCT] mean corpuscle haemoglobin [MCH] mean corpuscle hemoglobin concentration [MCHC] hemoglobin[HGB] red cell distribution width [RDW] and serum cholesterol were measured and the relation between the viscosity of whole blood and these parameters were studied. The results obtained show a correlation between blood viscosity and eash of RBC's count [r= 0.78] HCT [r = 0.51] and RDW [r = 0.537] while the other blood parameters are not correlated