[Molecular studies on avian leukosis virus subgroup I [ALV-J] infections in grandparent flocks in Iran]

Subgroup J Avian Leukosis Virus [ALV-J] was isolated in the late 1980s from meat-type chickens in the United Kingdom. ALV-J with exception of some acute variants, cause myeloid leukosis in meattype chickens with long incubation period. In this study while the status of Avian Leukosis Virus Subgroup J in six different strain of broiler grandparent flocks of Iran evaluated, the evaluation of different primers and some molecular methods also were done. For this reason, 100 blood samples, which had EDTA, 100 mixed of white blood cells and plasma and 100 feather pulps were collected from one farm of each broiler grandparent strain. PCR and nested PCR were methods that used for the study. In molecular study before the extraction of DNA from samples; In order to decrease the number of samples, 10 mixed of white blood cells and plasma, blood or feather pulp pooled. In molecular study with PCR all of samples were negative, but in nested PCR reaction, from six broiler strains, five strains infected to ALV-J. The results indicate that the most of broiler strains in Iran have been infected to ALVJ. The results also indicate that we can simply use pooled samples for the study of ALV-J in a flock by nested-PCR test

Identification of Isolated fungi from different lesions of birds with aspergillosis referred to mycology research center, faculty of veterinary medicine, university of Tehran [1991-2003]

In this study, the samples of 263 birds suspected to Aspergillosis were examined by direct microscopy, culture and histopathological methods. Of 263 affected birds, 185[85.3%], 23[79.3%], and 12[70.6%]were infected to pulmonary, ocular, and skin Aspergillosis, respectively. The most frequent aspergillus species were A.fumigatus[70.4%], and then A.flavus[22.7%], A.niger[3.6%], A.terreus[2.3%], and A.ustus[0.9%]. Chickens were the most affected birds[85%]. Aspergillus flavus was the most frequent pathogenic isolate in canary[61.5%]. Young birds were most involved with pulmonary lesions[96.1%], whereas the ocular and skin lesions were mainly observed in adults. Regarding to high prevalence of different Aspergillus species in the environment and contaminated feedstuffs, it should be considered to plan a program to prevent the growth of these pathogens in breeding poultry

[Isolation and identification of Pasteurella multocida inbreeder stocks]

To determine the rate of infection in breeder stocks.A total of 11 breeder stocks.Pharyngeal swab samples from 11 farms, 3 flocks of healthy birds, 3 recovered flocks, 3 flocks with chronic disease and 2 infected flocks, were taken. The samples were transferred to the laboratory in transport media.and tested on culture media. The positive cases were identified and the p. multocida was confirmed by differential tests. Pasteurella multocida was not isolated from either healthy or recovered birds. The organism was neither isolated from the pharyngeal swab samples of chronic form. In flocks, which showed acute form, p.multocida was isolated from both the pharyngeal swabs or carcasses. The results in this survey indicate that the healthy birds are not carrier of p. multocida and the diseases spread by infected birds, rodents, water and feed