Effect of obesity on lipolytic activity of adipose tissue in male rats

Obesity has become a major problem in the modern world; it is affected genetically and by lifestyle. Since obesity is associated with multi co-morbidities like dyslipidemia, hypertension, and metabolic syndrome, this work studied the effect of induced obesity on body weight, blood pressure, lipid profile, glycemic state and lipolytic activity of adipose tissue in male rats. Twenty male rats of a body weight ranging from 178 to 200 g were divided equally into two groups, one fed commercial rat chow as a control group [Cgp] and the other fed 10% saturated fat to induce obesity as experimental group [SFgp]. After 3 months the body weight, blood pressure, fasting blood glucose, insulin level, and lipid profile of both groups were measured and the lipolytic activity of adipose tissue was assessed by the amount of free glycerol released. Rats fed saturated fat for 3 months showed significant increase in birth weight, systolic blood pressure, TG, Cholesterol, LDL, fasting blood glucose and insulin levels by 55.3%, 15.86% 24%, 7.92%, 19.58% 20.5% and 26.25% respectively with a significant decline in HDL by 12.9%. Lipolytic activity of SC tissue in the presence of adrenaline decreased significantly by 15%, while in the presence of insulin it increased significantly by 33.33%. It showed a significant increase by 18% and 25.84% in the presence of adrenaline and insulin respectively in visceral adipose tissue. A p value less than 0.05 was considered statistically significant. obesity induced in male rats by high saturated fat diet showed a significant rise in BW and is associated with hypertension, dyslipidemia, hyperglycemia and insulin resistance. The adipose tissue of obese male rats showed a significant decrease in lipolytic activity of SC with a significant rise in the corresponding value in visceral adipose tissue

Effect of insulin resistance on lipolytic activity of adipose tissue in male rats

The excess usage of fructose as a sweetener that we all consume everyday in one way or another has raised the incidence of insulin resistance among the population which is associated with dyslipedemia, hypertension and obesity. This work studied the effect of induced insulin resistance on body weight, blood pressure, lipid profile, glycemic state and lipolytic activity of adipose tissue in male rats. 20 male rats of 129.4 g average body weight were divided equally into two groups. Both had free access to water. The control [Cgp] had pure water, the experimental group [Fgp] had water mixed with 25% of fructose to induce insulin resistance. After 3 months the body weight, blood pressure, fasting blood glucose, insulin levels, lipid profile of both groups were measured and lipolytic activity of adipose tissue was assessed by the amount of free glycerol released. Rats given fructose for 3 months showed significant increase in BW, systolic blood pressure, TG, Cholesterol, LDL, fasting blood glucose and insulin levels by 28.7%, 13.9% 23.9%, 3%, 5.61% 115.49% and 232.8% respectively with a significant decline in HDL by 5.98%. Lipolytic activity of SC and visceral adipose tissue in presence of adrenaline increased significantly by 55.25%, and 78.63% respectively, which runs in parallel with the results obtained in presence of insulin as it showed a significant rise in both SC and visceral adipose tissue by 109.3% and 167.12 respectively. data were statistically significant at p<0.05. Insulin resistance induced in male rat by high fructose consumption showed a significant rise in BW and is associated with hypertension and dyslipidemia with significant rise in lipolytic activity of both SC and visceral adipose tissue