RESUMEN
This study was conducted to determine some biochemical alterations of uterine flushings in relation to uterine condition. For this reason, uterine flushings from 50 repeat-breeder buffalo-cows and 10 apparently normal buffalo-cows [control] were collected via Foley's balloon catheter using 40 ml sterile normal saline. Total protein concentration [T.P.], alkaline phosphatase [ALP], lactate dehydrogenase [LDH], aspartate aminotransferase [AST] and alanine arnino transferase [ALT] activities of the collected uterine flushings were determined. The mean values of T.P., ALP, LDH, AST and ALT of uterine flushings collected from repeat-breeder buffalo-cows were significantly higher than those of apparently normal buffalo-cows. This study indicates that the biochemical alterations [total protein and enzymatic activities] of uterine flushings can be used for diagnosis of endometritis along with microbiological isolations