[Histomorphology of the olfactory mucosa and spinal tissue sparing following transplantation in the partial spinal cord injury in rats]

Nowadays, cellular and tissues transplant has become the focus of attention for spinal cord injury. It has been shown olfactory nerve cells or olfactory mucosa whi have more efficient on nervous tissue repair and they have been more studied in experimental study. Furthermore, they were used in a few clinical centers for spinal defect. But mucosa tissue and spinal tissue have different structure and there is doubt about the integration of mucosa tissue in nervous tissue. Thus, in this research the morphology and the effect of the fetal olfactory mucosa [FOM] on spinal tissue sparing were studied after transplanted into the spinal cord hemisection in rats. This experimental study was conducted at Iran university of Medical Sciences in 2008. Of thirty eight female Sprague-Dawley [200-250g] rats twenty- eight were spinally hemisected at the L1 spinal level and were randomized into two groups of 14 animals. Treatment group received FOM graft and the control received fetal respiratory mucosa graft [FRM]. The other animals received surgical procedure without spinal cord injury as a sham group. The morphology of the transplant region and spinal tissue sparing was examined histological eight weeks after transplantation. The collected data was analyzed by the SPSS software using ANOVA and the morphology of the transplant region were studied by light microscope. Histological study showed that the both mucosa tissues could not integrate with the parenchyma of the spinal tissue. Although the FOM were fused more then the FRM with the host tissue but clear boundary was seen at the graft-host interface. The mean spinal tissue sparing of the treatment group increased a little compare to the control but a significant difference was not apparent whereas, the spinal tissue sparing in treatment and control groups compare to the sham group decreased significantly [P <0.05]. Transplantation of the mucosa tissue directly, into the spinal cord injury was created different cytoarchitecture with spinal tissue and FOM partially preserving tissue sparing

Dendritic spine changes in medial prefrontal cortex of male diabetic rats using golgi-impregnation method

Neuropathy is one of the major complications contributing to morbidity in patients with diabetes mellitus. The effect of diabetes on brain has not been studied so much and no gross abnormality has been found in the central nervous system of patients with diabetic neuropathy. This study was conducted to evaluate the time-dependent structural changes in medial prefrontal cortex of male diabetic rats using Golgi-impregnation method. Male Wistar rats were randomly divided into the control and diabetic groups. For induction of diabetes, a single dose of streptozotocin [60 mg/kg] was injected intraperitoneally. At the end of the first and second months, the rats were transcardially perfused with a solution of phosphate buffer containing paraformaldehyde and Golgi-impregnated method was used to evaluate the changes of dendritic spines in medial prefrontal cortex. There was a significant reduction in the mean density of pyramidal neuron dendritic spines in the layers II and III of medial prefrontal cortex only after 2 months in the diabetic group compared to age-matched controls [P<0.05]. Diabetes induces a reduction in the spine density of apical dendrites of medial prefrontal cortex only in two-month diabetic rats