Saliva, supragingival biofilm and root canals can harbor gene associated with resistance to lactamic agents

This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without primary endodontic infections. Saliva (S) and supragingival biofilm (SB) were collected from three patient groups: Group I – no endodontic infection (n = 15); Group II – acute endodontic infection (n = 12); and Group III – chronic endodontic infection (n = 15). Root canal (RC) samples were collected from Groups II and III. The presence of P. intermedia, P nigrescens, P. tannerae and cfxA/cfxA2 gene was assessed by PCR. The cfxA/cfxA2 gene was not detected in all environments within the same patient. The cfxA/cfxA2 gene was present in 23.81% of S samples, 28.57% of SB samples, and 7.41% of RC samples. Prevotella species were detected in 53.97%, 47.62% and 34.56% of the S, SB, and RC samples, respectively. P. intermedia had a high frequency in saliva samples from Group 3. Saliva samples from Group 1 had higher detection rates of P. nigrescens than did Groups 2 and 3. Patients without endodontic disease had high frequencies of P. nigrescens in the SB samples. The presence or absence of spontaneous symptoms was not related to the detection rates for resistance genes in the RC samples. Saliva, supragingival biofilm and root canals can harbor resistant bacteria. The presence of symptomatology did not increase the presence of the cfxA/cfxA2 gene in the supragingival biofilm and inside root canals.

Avaliação da necessidade de tratamento periodontal e índice de cárie radicular numa população de Anápoli - GO / Periodontal treatment needs and root caries index of a Anapolis - GO population

O objetivo deste estudo foi avaliar a necessidade de tratamento periodontal e o Índicede Cárie Radicular (ICR) de pacientes dentados com 40 ou mais anos que procuraram tratamento noCurso de Odontologia da UniEVANGÉLICA. Foram examinados 140 pacientes. Para a análise danecessidade de tratamento periodontal, foi utilizado o Índice PSR modificado. A recessão gengivalfoi medida em milímetros, e foi calculado o Índice de Cárie Radicular (superfícies radicularescariadas ou restauradas/total de superfícies radiculares expostas x 100%). Observou-se diminuiçãodo número médio de dentes remanescentes (40-49 anos: 20,1; 70 anos ou mais: 12,3); aumento daextensão e severidade das recessões gengivais (40-49 anos: 56,01% e 1,74 mm; 70 anos ou mais:91,89% e 3,72 mm, respectivamente) e aumento no Índice de Cárie Radicular (40-49 anos: 5,4%;70 anos ou mais: 10%) em função da idade. Entretanto, a necessidade de tratamento periodontal(PSR graus 3 e 4 verdadeiros) foi maior nos grupos de faixa etária mais baixa. Concluiu-se que,com a progressão da idade, houve redução da necessidade de tratamento periodontal e aumentono Índice de Cárie Radicular.

The aim of this study was to evaluate the periodontal treatment needs and the RootCaries Index (RCI) from 40 years old or elder patients of UniEVANGELICA School of Dentistry.140 dentated patients were examined. For Periodontal Treatment Needs evaluation it was used amodified Periodontal Screening and Recording (PSR) index. Gingival recession was measuredin millimeters and the Root Caries Index was calculated (decayed or filled root surfaces/totalexposed root surfaces x 100%). It was observed a reduction in remaining teeth (40-49 yearsold: 20.1; 70 years old or older: 12.3); increased severity and extension of gingival recession(40-49 years old: 56,01% and 1.74 mm; 70 years old or older: 91.89% and 3.72 mm, respectively)and increased Root Caries Index (40-49 years old: 5.4%; 70 years old or older: 10%) related toaging. However, the Periodontal Treatment Needs (true 3 and 4 PSR scores) were higher at lowage patients. It was concluded that, with aging, there were less Periodontal Treatment Needs, whilethe Root Caries Index increased.