RESUMEN
Abstract The aim of this study is to evaluate the expression of cytokines in response to mineral trioxide aggregate (MTA) plus selenium in germ-free mice with experimental furcal perforation. The first left maxillary molar was opened, and the furcal area was perforated and treated with post-MTA-Se (experimental group). The same surgical intervention was performed for the maxillary right first molar, which was treated with MTA (control group). Fifteen mice were sacrificed 7, 14, and 21 days after furcal perforation, and periapical tissue samples were collected. The mRNA expression levels of the cytokines TGF-β, TNF-α, IFN-γ, HPRT, IL-10, IL-4, RANK, RANKL, IL-1, and IL-17 were assessed by using real-time polymerase chain reaction. In the experimental group, at 21-days post-MTA-Se sealing, the mRNA levels of TNF-α and IL-10 were upregulated compared with those in the control group (p < 0.05). Futher assessment revealed basal mRNA expression levels of IL-1α, IFN-γ, RANK, RANKL, IL-17A, IL-4, and TGF-β, over long experimental times, in both the experimental and control groups (p > 0.05). In conclusion, MTA+Se sealing favoured increased expression of IL-10 and TNF-α at later time points (day 21).
Asunto(s)
Animales , Masculino , Femenino , Óxidos/farmacología , Materiales de Obturación del Conducto Radicular/farmacología , Selenio/farmacología , Citocinas/análisis , Silicatos/farmacología , Defectos de Furcación/tratamiento farmacológico , Compuestos de Calcio/farmacología , Compuestos de Aluminio/farmacología , Cavidad Pulpar/lesiones , Tratamiento del Conducto Radicular/métodos , Factores de Tiempo , Reproducibilidad de los Resultados , Resultado del Tratamiento , Defectos de Furcación/inmunología , Cavidad Pulpar/efectos de los fármacos , Cavidad Pulpar/inmunología , Combinación de Medicamentos , Reacción en Cadena en Tiempo Real de la Polimerasa , Diente Molar/efectos de los fármacos , Diente Molar/lesionesRESUMEN
A infecção dos sistemas de canais radiculares levará à consequente indução de uma lesão periapical, estando muitos mediadores inflamatórios envolvidos nesse processo. A IL-17 tem demonstrado impacto no processo de remodelação óssea, especialmente em casos de artrite, apresentando-se em altos níveis. Contudo, pouco se sabe a respeito do seu real papel na patogênese das periapicopatias. Neste estudo, procurou-se avaliar o papel da IL-17A na reabsorção óssea perirradicular, em infecções endodônticas experimentais em camundongos C57BL/6 e IL-17RA KO, bem como o efeito dessa infecção na artrite reumatóide experimental. Para se induzir a infecção endodôntica, cepas padrão de Porphyromonas gingivalis, Prevotella intermedia e Fusobacterium nucleatum foram inoculadas nos canais radiculares do primeiro molar superior esquerdo desses animais...