RESUMEN
yielded an average particle size of 120 nm with 70% encapsulation-efficiency. In vitro release profile of NP-OP showed sustained release of OP for 21 days. In vivo anti-fertility studies were conducted in marmosets. Results indicated that control animals conceived in the same cycle while two of three treated animals failed to conceive in treatment cycle. The in vivo studies thus corroborate with in vitro release of OP, demonstrating its anti-fertility activity in 66% of animals.
Asunto(s)
Animales , Callithrix/fisiología , Proteínas Portadoras/administración & dosificación , Proteínas Portadoras/química , Anticoncepción , Femenino , Humanos , Nanopartículas/administración & dosificación , Nanopartículas/química , Folículo Ovárico/química , Tamaño de la Partícula , Fragmentos de Péptidos/administración & dosificación , Fragmentos de Péptidos/química , Polímeros/administración & dosificación , Polímeros/químicaRESUMEN
A synthetic nonapeptide, which is C-terminal sequence of 94-amino acid of prostatic inhibin peptide was tested for progesterone and estrogen secretion by mouse granulosa cell cultures. Nonapeptide suppressed the progesterone and estrogen synthesis, the magnitude of suppression was highest at 5 ng dose level for progesterone and 50 ng dose level for estradiol. The study suggests that, nonapeptide exerts its effect by impairing the binding of FSH to granulosa cell receptors.
Asunto(s)
Animales , Células Cultivadas , Relación Dosis-Respuesta a Droga , Femenino , Hormona Folículo Estimulante/metabolismo , Hormonas Esteroides Gonadales/biosíntesis , Gonadotropinas Equinas , Células de la Granulosa/efectos de los fármacos , Inhibinas/farmacología , Ratones , Fragmentos de Péptidos , Progesterona/biosíntesisRESUMEN
Synthesis and biological profile of a decapeptide analogue, [Tyr85, Cys(Acm)87]85-94 of human seminal plasma inhibin (HSPI) are described. The peptide suppressed the circulatory levels of follicle stimulating hormone (FSH) in adult male rats. No change in the levels of luteinizing hormone (LH) and prolactin (Prl) was observed. Whereas the peptide suppressed the release of both FSH and LH in vitro. This decapeptide is the smallest peptide reported so far to have FSH suppressing activity.