Evaluation of thyroid disorders on the basis of SDS-page electrophoresis

SOS-Page electrophoresis identifies the various protein fractions under different conditions. This property of the process was utilized to see the pattern of protein fractions among patients suffering from thyroid disease. A group of 1170 subject, comprising of nearly equal proportions of euthyroid, hypothyroid and hyperthyroid individuals, was studied. Most of the subjects were out-patients of Shaikh Zayed Hospital, Lahore. Random blood samples were taken from the subjects usually in the morning between 0800 - 1200 hours and after processing, the serum was stored at -80[C]. These aliquots were used for thyroid profile, bio-chemical analysis and SDS-PAGE electrophoreses. The serum samples of healthy / euthyroid samples showed 13 major protein fractions ranging in molecular weight of 321 to 28 kDa whereas 12 major protein fractions were detected in patients suffering from thyroid diseases. The fractions were found to be enhanced due to increased capillary permeability and increased interstitial fluid. By contrast, markedly reduced fractions were found among hyperthyroids probably due to the factors of negative nitrogen balance, loss of weight, muscle wasting, weaknesses mild hypo-albuminemia and increased anabolism of protein. The findings presented in this study suggest that SDS-PAGE may be a useful tool in the diagnosis of thyroid disorders

Analytical study of deliveries in young females at shaikh zayed hospital, lahore

In 1988, 285 young female subjects who were under the age of thirty at the time of deliveries were studied at Shaikh Zayed Hospital, Lahore. The encouraging aspect of this study is that those under the age of 25 did not suffer from prolonged labour, and the young female is at no risk

Role of hormonal stability in the blood for thyroid hormones at Shaikh Zayed Hospital Lahore

The study was carried out at the National Health Research Complex, Pakistan Medical Research Council at Shaikh Zayed Hospital, Lahore. Blood/Serum samples were collected from 60 healthy volunteers with an age range of 20 to 55 years and over. All serum samples were analyzed in duplicate to determine the stability of four hormones i.e. TT3, TT4, TSH and FT4 and measured with Amerlex RIA kits [Amersham International Ltd., UK]. The purpose of this study was to see the effect of time and the stability and concentration of hormones in the serum samples if they were not separated for periods upto one week. It was found that most of the hormones were stable when stored in this manner, although some changes were observed but they were statistically insignificant. The changes observed would not have any bearing on the clinical interpretation of the results

prospective analysis of thyroid profile with the emphasis on Grave's disease

This study suggests that there is no relationship between the clinical assessment of disease severity and serum levels of thyroid hormone in untreated Grave's disease. Potential variables such as cellular responsiveness to thyroid hormone and/or catecholamines and their resultant effect to produce hyperthyroid symptoms could contribute to the lack of a linear relationship between serum thyroid hormone levels and severity of thyrotoxic symptoms