Chromosomal analysis of "failed-fertilized" human oocytes resulting from in-vitro fertilization and intracytoplasmic sperm injection.

To evaluate the incidence of chromosomal abnormalities in "failed-fertilized" oocytes derived from in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) procedure, a cytogenetic analysis was performed on 164 IVF and 64 ICSI oocytes. One hundred and eleven (67.7%) of the IVF and 56 (87.5%) of the ICSI oocytes were successfully karyotyped. Of 111 IVF oocytes, 73 (65.8%) exhibited normal haploid and 38 (34.2%) were abnormal. The abnormalities included 25 aneuploid (22.5%) (7 hyperhaploid and 18 hypohaploid), 11 diploid (9.9%) and 2 structural anomalies (1.8%). Of 56 ICSI oocytes, 52 (92.8%) were normal haploid and only 4 (7.2%) were aneuploid, with 2 hyperhaploid and 2 hypohaploid. The sperm nuclei were observed in 43 IVF oocytes (38.7%), composed of 38 (34.2%) premature chromosome condensation (PCC) and 5 (4.5%) decondensed sperm heads. Evidence of successful sperm delivery was found in all 56 ICSI oocytes; 25.0 per cent (14/56) showed PCC, 17.9 per cent (10/56) showed decondensed sperm heads, and 57.1 per cent (32/56) showed intact sperm heads. This study suggested that about one-third of unfertilized oocytes exhibited chromosomal abnormalities. The difference of aneuploidy between IVF and ICSI oocytes needs further studies analysing a larger number of oocytes.

Chromosome analysis of unfertilized oocytes after in vitro fertilization program: a preliminary report.

To assess the contribution of chromosome anomalies to high failure of in vitro fertilization (IVF), a total of sixty three eggs from 37 women participating in the IVF program were cytogenetically investigated. The mean age of the oocytes donors was 36.8 years. Chromosome karyotype was obtained in 30 of unfertilized oocytes: 16 oocytes (53.3%) had a normal haploid chromosome complement; 5 (16.7%) were hypohaploid; 3 (10.0%) were hyperhaploid; 3 (10.0%) were diploid, 3 (10.0%) were polyploid. It may explain for early pregnancy loss, low pregnancy rate after in vitro fertilization.

Prenatal diagnosis of genetic disorder by aminotic fluid cell culture.

Eighty one samples of prenatal diagnosis for genetic disorder by amniotic fluid cell culture were studied. The study period was from January 1984 to 28 February 1987. Amniotic fluid cell culture were analyzed from 18 women who had previous child with Down’s Syndrome, a cases in which the family and congenital anomalies, 53 cased of advanced maternal age (> 35 years old) and 2 cases of miscellaneous (habitual abortion, X-ray) 2 cases. The success rate of the culture was 96.2 per cent. This study found the abnormality of amniotic Fluid cell culture in 3 cases. Two cases were Down’s Syndrome and one case was Klinefelter’s Syndrome. These three cases were proved by chromosome analysis of skin and lung culture.

Phenylketonuria in mother having previous mental retarded child.

Phenylketonuria (PKU) is an inborn deficiency of the enzyme L-phenlalanine hydroxylase resulting in raised serum phenylalanine concentration and mental retardation. This study was performed on pregnant women who had previously given birth to mentally retarded children and suspected of having phenlketonuria. The phenlalanine level in amniotic fluid of this group was compared with normal pregnant women. The result showed no statistical difference (p > 0.05). The reference values of serum phenlalanine in Thai normal pregnant women were also studied. The result of the first, second and third were 1.6 + 0.8 mg/dl, 1.8 + 0.9 mg/dl and 1.8 + 0.6 mg/dl respectively.