Assessment of Leuconostoc spp. invasion in standing sugarcane with cracks internode

Aim: The post-harvest deterioration is most vexing problems of sugar industry and these losses due to Leuconostoc spp. cause enormous depreciation in cane tonnage and recovery. Thus, the objective of this study was to assess the invasion of Leuconostoc spp. in sugarcane. Methodology: Single internode is divided in three layers, viz., rind, first half and second half. Leuconsotoc spp. was grown and identified on specific growth media using juice from three portions of both the internodes (with cracks and without cracks) separately. Total Leuconostoc load, bacterial characterization, total soluble solids (TSS), reducing sugars (RS) and dextran were also determined. Results: The results revealed that cracked portions were incurred with Leuconostoc, however, rind layer had highest load followed by first half than the second half. More RS, high TSS and dextrans were quantified in cracked internode than without crack internode. Rind layer reported to have high RS, TSS and dextrans than the other portions. Interpretation: Canes possessing cracks had higher invasion of Leuconostoc spp., RS and dextran content than the ones lacking cracks leading to higher loss in sucrose content. Thus, there is a need to control these bacteria in sugarcane, especially in standing canes.

Restoration of liver mass after partial hepatectomy - implications for living donor liver transplantation

Abstract In living donor liver transplantation; the recipient liver undergoes more rapid regeneration than the remnant liver in the donor. In this study we investigated the factors which may be responsible for the difference in the regenerative response between the donor and the recipient. Long Evans rats were subjected to either partial hepatectomy (PH) or sham operation (SH) and were treated with liver cytosol (C) and cyclosporine (Cy). The rats were sacrificed at 24; 48; 72 and 96 hours and 1 and 2 weeks postoperatively. The livers were removed to determine the liver weight/body weight (LW / BW) ratio and the mitotic index. The mitotic index; serum aspartate transferase (AST) and serum alanine transferase (ALT); although unchanged in the SH groups; were increased in the rats treated with PH + C + Cy; and were greater than after PH only. However LW / BW ratios increased after PH but had returned to preoperative levels by 2 weeks. The changes in LW / BW ratio were not modified by the cytosol or cyclosporine

Hypoalbuminaemia in brain-dead donors for liver transplantation: transplantation

Liver transplantation has become established as the treatment of choice for most patients with end-stage liver disease and is performed on a routine basis in most major centres throughout the world. The majority of donors for liver transplantation are brain-dead cadaver donors following either a severe head injury or a massive intracranial haemorrhage. Potential liver donors undergo a rigid screening process before being accepted. This includes a thorough clinical examination to assess the haemodynamic status of the donor and to exclude any overt evidence of liver disease. Blood samples are also taken for viral studies to exclude HIV infection and hepatitis B and C infection; and for liver function tests to exclude liver disease or liver injury. Over the years we have noted that our liver donors often had low serum albumin levels; although this has not been formally documented. A review of the literature revealed that hypoalbuminaemia associated with severe head injury has been documented previously. However the impact of brain death on serum albumin levels has not been studied previously. The present study was therefore undertaken to document serum albumin levels in brain-dead cadaver donors

Restoration of liver mass after partial hepatectomy - implications for living donor liver transplantation : transplantation

In living donor liver transplantation; the recipient liver undergoes more rapid regeneration than the remnant liver in the donor. In this study we investigated the factors which may be responsible for the difference in the regenerative response between the donor and the recipient. Long Evans rats were subjected to either partial hepatectomy (PH) or sham operation (SH) and were treated with liver cytosol (C) and cyclosporine (Cy). The rats were sacrificed at 24; 48; 72 and 96 hours and 1 and 2 weeks postoperatively. The livers were removed to determine the liver weight/body weight (LW / BW ) ratio and the mitotic index. The mitotic index; serum aspartate transferase (AST) and serum alanine transferase (ALT); although unchanged in the SH groups; were increased in the rats treated with PH + C + Cy; and were greater than after PH only. However LW / BW ratios increased after PH but had returned to preoperative levels by 2 weeks. The changes in LW / BW ratio were not modified by the cytosol or cyclosporine