Effect of selected insecticides on SF9 insect cell line

The toxic effect of three insecticides: dimethoate [organophosphate insecticide], acetamiprid [neonicotinoid insecticide] and deltamethrin [pyrethroid insecticide] were evaluated in vitro on cultured Sf9 cell line. Cell growth inhibition was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide [MTT] assay. Regression Analysis was used to estimate the 20% inhibition of cells growth [IC[20]]. The IC[20] values obtained for deltamethrin, acetamiprid and dimethoate were: 46.8, 61.6 and 68.9 micro M, respectively. The proportion of phagocytic cells was positively correlated with the applied concentrations of the insecticides