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BACKGROUND: Incidence of cardiac pathological changes is high under acute cerebral damage, which affects its prognosis. The massive increase of plasma monoamine transmitters might be one of the key reasons of the dependent effects of the heart after cerebral apoplexy.OBJECTIVE: To investigate the effects of monoamine transmitters on the cardiac injury in cerebral hemorrhage in rats for the exploration of the mechanism for the generation and development of cerebral-cardiac syndrome(CCS).DESIGN: A prospective, randomized and controlled study using experimental animals as subjects.MATERIALS: Study was conducted in the Laboratory of Neurology of Daping Hospital. Seventy healthy Wistar rats in either gender with a body mass between 250 g and 300 g were obtained from the animal center of Daping Hospital affiliated to Third Military Medical University of Chinese PLA.INTERVENTIONS: 1 μL of normal saline containing collagenase(l μ/μL) and heparin(7 μ/μL) was injected into the right caudate nucleus of the rats to establish cerebral hemorrhage animal model, while 1 μL of normal saline was injected into the rats of control group.MAIN OUTCOME MEASURES: Plasma noradrenaline (NE) content was analyzed by high-potent electrochemical method, changes of serous creatine kinase-MB (CK-MB) were analyzed by enzyme reaction rate method.RESULTS: Both plasma NE and serous CK-MB significantly elevated at 6 hours since the formation of hematoma in the brain of the rat, which were (2. 782 ± 0. 244) μg/L and(3. 504 ± 0. 742) μkat/L respectively. Plasma NE and serous CK-MB most significantly increased at 24 hours, the peak of hematoma after cerebral hemorrhage, which were(3. 517 ± 0. 200) μg/L and(5. 474 ± 1. 540) μkat/L respectively. NE and CK-MB gradually decreased afterwards, and plasma NE returned to preoperative level at 72 hours [ (1. 663 ±0. 094) μg/L] while serous CK-MB still higher than preoperative level [ (2. 713 ± 0. 526) μkat/L].CONCLUSION: Peripheral NE content elevation might participate the generation and development process of CCS.
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Objective To explore the changes and significance of somatostatin (SS) and acetylcholine (Ach) contents in vascular dementia rats. Methods Model of vascular dementia rats was established. Active avoidance responase (AAR) ratio was used as the index for the judgement of the learning and memory abilities of vascular dementia rats. The SS and Ach contents in different cerebral sections of rats with and without intervention of huperzine A were determined by radioimmunoassay. Results A remarkable decrease in SS and Ach contents was found in vascular dementia rats, which could be reversed by intervention of huperzine A. Conclusion The decreased SS content in cerebrum after ischemia is related with vascular dementia. SS may be involved in the pathological process of vascular dementia through the mutual regulation with Ach.
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Objective To detect the expression of TNF α and ICAM 1 in cerebral ischemia reperfusion injury. To explore their probable modulation mechanism.Methods Immunohistochemistry was used to examine the expression of TNF α and ICAM 1.The MPO activity was determined by spectrophotometry.Result The expression of TNF α and ICAM 1 were obviously rised after cerebral ischemia reperfusion (P< 0.05).Conclusion The up regulation of TNF α and ICAM 1 after cerebral ischemia reperfusion induced the accumulation of leukocytes, which involved in ischemia reperfusion injury mechanism. TNF α play an important role in expression of ICAM 1.
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Objective To study the relationship between atherosclerotic plaques in carotid artery and ischemic cerebrovascular diseases. Methods The extracranial carotid arteries (ECA) and middle cerebral artery (MCA) of 54 patients with transient ischemic attack (TIA) or cerebral infarction (CI) were examined with doppler ultrasound. The distribution of atherosclerotic plaque, degree of stenosis and ultrasounic classification of ECA and the mean velocity of blood flow in MCA were examined. Results ①Stenosis over middle-grade on asymptomatic side in extracranial internal carotid artery (EICA) in group of patients with TIA was significantly higher than symptomatic side(P<0.01). Stenosis over high-grade on asymptomatic side in ELCA in group of patients with CI was significantly higher than symptomatic side (P<0.01). ②Flat and soft plaque are most common in group of patients with TIA or CI, then are hard and ulcerative plaques. Incidence of soft plaques on asymptomatic side in group of patients with TIA or CI are significantly higher than symptomatic side (P<0.01); ③Among the group of patients with CI, mean velocity of MCA decreased on asymptomatic side in 31 cases (68.9%), and significantly higher than symptomatic side (P<0.01). Conclusion Atheroclerotic plaques in carotid artery and intracranial hemodynamic characteristics are the important risk factors for ischemic cerebrovascular diseases. These findings have important values in predicting subsequent TIA or CI in asymptomatic subjects.
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Objective To study the effect and the mechanism of sibelium on apoptosis and transcription of bcl 2 clan and interleukin 1? converting enzyme(ICE) gene in limbic system after rats' middle cerebral artery occlusion(MCAO).Methods MCAO model was made, and determined the dynamic changes on the cell counts of apoptosis, bcl 2 clan and ICE gene positive transcription in temple ortex, hippocampus and hypothalamus in the controls of normal saline,sibelium 50 ?g intervenient group and sibelium 100 ?g intervenient group during rats MCAO or reperfusion.Results The apoptosis counts of every times in sibelium 100?g intervenient group was significant lower than another two groups( P 0.05).The bcl 2 gene positive transcription cell counts at every times in sibelium 100?g intervenient group were significant higher than another two gruops( P
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In order to study the relationship between acute brain hypoxia and epilepsy,acute brain hypoxia was induced in 12 rabbits and the clinical manifestations were observed.It was found that the occurrence of epilepsy was not in close relation with the severity of brain hypoxia.Pathological examination of the brain of the rabbits revealed that there was local ischemic change of the neurons of the cortex and necrosis in the subcortical region in a few of the rabbits.The mechanism of the results was discussed.