Effect of grape seed extract-containing phosphoric acid formulations on bonding to enamel and dentin

Abstract The aim was to evaluate the effect of 2% grape seed extract (GSE) containing phosphoric acid (PhA) on the bond strength to enamel and dentin. The control group was 37% PhA. The following three PhA formulations with 2% GSE and 20% ethanol were obtained: GSE5 = 5% PhA; GSE10 = 10% PhA; and GSE20 = 20% PhA. The enamel and dentin surfaces of molars were etched with the acid solutions, followed by Scotchbond Multi-Purpose adhesive and composite resin application. The tensile bond strength (TBS) test evaluated the bond to enamel after 24 h, and the microtensile bond strength (μTBS) test evaluated the bond to dentin after 24 h and 12-month water storage. Etched enamel and dentin were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM), respectively. The TBS data were submitted to one-way ANOVA, while µTBS data were submitted to two-way ANOVA and Tukey's test (α = 0.05). The TBS (MPa) to enamel did not significantly differ among the control (48.1 ± 15.7), GSE5 (46.1 ± 9.6), GSE10 (49.8 ± 13.6) and GSE20 (44.1 ± 11.9) groups (p = 0.537). The µTBS (MPa) to dentin of the control (28.4 ± 14.4) and GSE20 (24.1 ± 8.1) groups were significantly higher than those of the GSE5 (16.8 ± 7.4) and GSE10 (17.5 ± 6.6) groups at 24 h (p < 0.006). After 12-month storage, only GSE5 (21.0 ± 7.8) and GSE10 (17.6 ± 8.0) did not show significantly decreased μTBS (p > 0.145). SEM micrographs showed a shallower enamel etching pattern for GSE5. AFM images showed the formation of collagenous globular structures for GSE5 and GSE10. The different acid solutions did not influence the TBS to enamel, and the µTBS to dentin was stable over time when dentin was etched with GSE5 and GSE10.

Relined fiberglass post: an ex vivo study of the resin cement thickness and dentin-resin interface

Abstract The aim of this study was to evaluate the thickness of resin cements in the root thirds when using conventional fiberglass posts (CP) and relined fiberglass posts (RP) in weakened roots and to evaluate the morphological characteristics of the dentin-resin interface. Forty human maxillary anterior teeth had the crown sectioned below the cemento-enamel junction. The canals were endodontically treated and weakened with diamond burs. Teeth were divided into four groups (n = 10): Group 1 - CP + RelyX ARC; Group 2 - CP + RelyX U200; Group 3 - RP + RelyX ARC; and Group 4 - RP + RelyX U200. Prior to luting, 0.1% Fluorescein and 0.1% Rhodamine B dyes were added to an adhesive and resin cement, respectively. Slices were obtained from the apical, middle, and cervical thirds of the root. Confocal laser scanning microscopy images were recorded in four areas (buccal, lingual, mesial, distal) of each third. In each area, four equidistant measures of the resin cement were made and the mean value was calculated. The interface morphology was observed. The data were submitted to three-way ANOVA and Tukey's test (α = 0.05). The interaction between fiberglass posts, resin cement, and root thirds was significant (p < 0.0001). The resin cement thicknesses were significantly lower for RP in comparison with CP, except in the apical third. There was no significant difference between the resin cements for RP. There was formation of resin cement tags and adhesive tags along the root for RP. RP favored the formation of thin and uniform resin cement films and resin tags in weakened roots.

Influência do uso do laser de Nd: YAG na resistência de união de materiais resinosos à dentina / YAG laser influence on bond strength of adhesive resins to dentin

The aim of this study was to evaluate the influence of Nd:YAG laser on microtensile bond strength (μTBS) of different adhesive materials to human dentin. Eighteen human third molars were divided into 6 groups. Group 1 – adhesive system, Adper Single Bond 2 (SB); Group 2 – adhesive system, Clearfil SE Bond (CSEB); Group 3 – self-adhesive resin luting cement, RelyX Unicem; Group 4 – irradiation with Nd:YAG laser and application of SB; Group 5 – irradiation with Nd:YAG laser and application of CSEB; Group 6 – irradiation with Nd:YAG laser and application of RelyX Unicem. A composite resin block was built on the dentin surfaces, and sticks with a cross-sectional area of ~0.80 mm2 were obtained. Twenty specimens per group were selected and subjected to μTBS testing in a universal testing machine. According to the analysis of variance and Tukey test (α = 0.05), the highest μTBS mean was obtained with CSEB (41.75 MPa) without laser irradiation, but this value was not statistically different from CSEB with laser irradiation (33.42 MPa). Mean μTBS of SB without (32.15 MPa) and with (35.38 MPa) laser irradiation did not differ statistically. The lowest μTBS values were obtained for RelyX Unicem, and the group without laser irradiation showed statistically higher values (14.50 MPa) than those found with laser irradiation (7.53 MPa). Nd:YAG laser did not significantly reduce bond strength of the adhesive systems under study, except for the self-adhesive resin luting cement.

O objetivo desse estudo foi avaliar a influência do laser Nd:YAG na resistência à microtração de diferentes materiais adesivos à dentina humana. Dezoito terceiros molares humanos foram divididos em 6 grupos. Grupo 1 – Adesivo Adper Single Bond 2 (SB), Grupo 2 – Adesivo Clearfil SE Bond (CSEB), Grupo 3 – Cimento resinoso autoadesivo RelyX Unicem, Grupo 4 – irradiação com laser de Nd:YAG e aplicação de SB, Grupo 5 – irradiação com laser de Nd:YAG e aplicação de CSEB e Grupo 6 – irradiação com laser de Nd:YAG e aplicação de RelyX Unicem. Um bloco de resina composta foi confeccionado sobre essas superfícies de dentina tratadas e os espécimes foram seccionados para obtenção de palitos de 0,80 mm2. Vinte espécimes foram selecionadas por grupo e submetidos a resistência adesiva à microtração (μTBS) em máquina de ensaio universal. De acordo com a análise de variância e teste de Tukey (α = 0,05), a maior média μTBS foi obtida com CSEB (41,75 MPa) sem irradiação por laser, esse valor não diferiu estatisticamente do CSEB com irradiação por laser (33,42 MPa ). A μTBS média de SB sem (32,15 MPa) e com (35,38 MPa) irradiação do laser não diferiram estatisticamente entre si. Os menores valores de μTBS foram obtidos para RelyX Unicem, sendo o valor sem irradiação por laser (14,50 MPa) estatisticamente superior ao com (7,53 MPa). O Laser de Nd: YAG não diminuiu significativamente a resistência de união dos sistemas adesivos estudados, apenas para o cimento resinoso autoadesivo.

Avaliação da topografia superficial de cerâmicas submetidas a diferentes tratamentos de superfície / Evaluation of surface topography of ceramics under different surface treatments

O objetivo deste estudo foi avaliar a topograa da superfície das cerâmicas Noritake, IPS e.max e Cercon quando empregado o jateamento com óxido de alumínio ou o condicionamento com ácido uorídrico. Foram confeccionadas seis amostras de cada cerâmica (10mm x 10mm x 0,5mm), sendo divididas em três grupos: Grupo 1: sem tratamento de superfície (controle); Grupo 2: jateamento com óxido de alumínio de 50 µm; Grupo 3: ácido uorídrico a 10%. As amostras foram metalizadas e observadas em microscópio eletrônico de varredura. O ácido uorídrico a 10% aumentou a irregularidade supercial das cerâmicas Noritake e IPS e.max, mas não alterou a topograa supercial da cerâmica Cercon. O jateamento com óxido de alumínio aumentou a irregularidade supercial das cerâmicas Noritake, IPS e.max e Cercon. Concluiu-se que o ácido uorídrico foi efetivo somente para as cerâmicas Noritake e IPS e.max, e o jateamento foi efetivo para as três cerâmicas.

The purpose of this study was to evaluate the surface topography of Noritake, IPS e.max and Cercon ceramics after treatment with either airborne aluminum oxide particle abrasion or hydrouoric acid etching. Six specimens of each ceramic (10mm x 10mm x 0,5mm) were fabricated and randomly divided into three groups: Group 1: without surface treatment (control); Group 2: airborne particle abrasion with 50-µm aluminum oxide; Group 3: 10% hydrouoric acid etching. The specimens were gold coated and examined using scanning electron microscopy. The 10% hydrouoric acid increased the surface irregularities of Noritake and IPS e.max ceramics, but did not change the surface topography of Cercon ceramic. Airborne particle abrasion with aluminum oxide increased the surface irregularities of Noritake, IPS e.max and Cercon ceramics. It was concluded that hydrouoric acid was effective only for Noritake and IPS e.max ceramics, and airborne particle abrasion was effective for the three ceramics.