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Braz. j. med. biol. res ; 42(2): 179-188, Feb. 2009. ilus, graf
Artículo en Inglés | LILACS | ID: lil-506879

RESUMEN

The immunomodulador glatiramer acetate (GA) has been shown to significantly reduce the severity of symptoms during the course of multiple sclerosis and in its animal model - experimental autoimmune encephalomyelitis (EAE). Since GA may influence the response of non-neuronal cells in the spinal cord, it is possible that, to some extent, this drug affects the synaptic changes induced during the exacerbation of EAE. In the present study, we investigated whether GA has a positive influence on the loss of inputs to the motoneurons during the course of EAE in rats. Lewis rats were subjected to EAE associated with GA or placebo treatment. The animals were sacrificed after 15 days of treatment and the spinal cords processed for immunohistochemical analysis and transmission electron microscopy. A correlation between the synaptic changes and glial activation was obtained by performing labeling of synaptophysin and glial fibrillary acidic protein using immunohistochemical analysis. Ultrastructural analysis of the terminals apposed to alpha motoneurons was also performed by electron transmission microscopy. Interestingly, although the GA treatment preserved synaptophysin labeling, it did not significantly reduce the glial reaction, indicating that inflammatory activity was still present. Also, ultrastructural analysis showed that GA treatment significantly prevented retraction of both F and S type terminals compared to placebo. The present results indicate that the immunomodulator GA has an influence on the stability of nerve terminals in the spinal cord, which in turn may contribute to its neuroprotective effects during the course of multiple sclerosis.


Asunto(s)
Animales , Femenino , Ratas , Encefalomielitis Autoinmune Experimental/tratamiento farmacológico , Inmunosupresores/uso terapéutico , Esclerosis Múltiple/tratamiento farmacológico , Plasticidad Neuronal/efectos de los fármacos , Péptidos/uso terapéutico , Médula Espinal/efectos de los fármacos , Astrocitos/efectos de los fármacos , Astrocitos/metabolismo , Astrocitos/ultraestructura , Encefalomielitis Autoinmune Experimental/metabolismo , Microscopía Electrónica de Transmisión , Neuronas Motoras/efectos de los fármacos , Neuronas Motoras/fisiología , Esclerosis Múltiple/metabolismo , Plasticidad Neuronal/fisiología , Ratas Endogámicas Lew , Médula Espinal/metabolismo , Médula Espinal/ultraestructura , Transmisión Sináptica/efectos de los fármacos , Transmisión Sináptica/fisiología , Sinaptofisina/análisis
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