Effects of probiotic fermented milk on biofilms, oral microbiota, and enamel

The aim of this study was to evaluate in vitro and in vivo the effects of 2 brands of probiotic fermented milk on biofilms, oral microbiota, and enamel. For the in situ experiment, ten volunteers wore palatine devices containing four blocks of bovine dental enamel over 3 phases, during which 20% sucrose solution, Yakult® (Treatment A), and Batavito® (Treatment B) were dropped on the enamel blocks. Salivary microbial counts were obtained and biofilm samples were analyzed after each phase. For the in vivo experiment, the same ten volunteers drunk Yakult® (Treatment C) and Batavito® (Treatment D) in two phases. Saliva samples were collected for microbial analysis after each phase. The in situ study showed that in comparison with Treatment A, Treatment B resulted in fewer total cultivable anaerobes and facultative microorganisms in biofilms, higher final microhardness, lower percentage change in surface hardness, and smaller integrated subsurface enamel hardness. In the in vivo study, Treatment D resulted in a reduction in the counts of all microorganisms. The results suggested that the probiotic fermented milk Batavito®, but not Yakult®, reduced the amount of oral microorganisms and mineral loss in bovine enamel.

Evaluation of some properties of fermented milk beverages that affect the demineralization of dental enamel

The aim of this in vitro study was to evaluate the erosive capacity of fermented milk beverages, as well as some of their properties that affect the demineralization of dental enamel (pH, buffering capacity, fluoride, calcium and phosphorus contents). Three different batches of 6 commercial brands of fermented milk beverages were analyzed. pH evaluation was accomplished using a potentiometer. The buffering capacity was measured by adding 1 mol L-1 NaOH. Fluoride concentration was assessed by an ion specific electrode after hexamethyldisiloxane-facilitated diffusion, and calcium and phosphorus concentrations were assessed by a colorimetric test using a spectrophotometer. Sixty specimens of bovine enamel were randomly assigned to 6 groups (n = 10). They were exposed to 4 cycles of demineralization in the fermented milk and remineralization in artificial saliva. Enamel mineral loss was determined by surface microhardness ( percentSMHC) and profilometric tests. The samples' pH ranged from 3.51 to 3.87; the buffering capacity ranged from 470.8 to 804.2 µl of 1 mol L-1 NaOH; the fluoride concentration ranged from 0.027 to 0.958 µgF/g; the calcium concentration ranged from 0.4788 to 0.8175 mgCa/g; and the phosphorus concentration ranged from 0.2662 to 0.5043 mgP/g. The percentSMHC ranged from -41.0 to -29.4. The enamel wear ranged from 0.15 µm to 0.18 µm. In this in vitro study, the fermented milk beverages did not promote erosion of the dental enamel, but rather only a superficial mineral loss.

The influence of residual salivary fluoride from dentifrice on enamel erosion: an in situ study

The objective of this study was to assess the salivary residual effect of fluoride dentifrice on human enamel subjected to an erosive challenge. This crossover in situ study was performed in two phases (A and B), involving ten volunteers. In each phase, they wore acrylic palatal appliances, each containing 3 human enamel blocks, during 7 days. The blocks were subjected to erosion by immersion of the appliances in a cola drink for 5 minutes, 4 times a day. Dentifrice was used to brush the volunteers’ teeth, 4 times a day, during 1 minute, before the appliance was replaced into the mouth. In phases A and B the dentifrices used had the same formulation, except for the absence (PD) or presence (FD) of fluoride, respectively. Enamel alterations were determined using profilometry, microhardness ( percentSMHC), acid- and alkali-soluble F analysis. The data were tested using ANOVA (p < 0.05). The concentrations (mean ± SD) of alkali- and acid-soluble F (µgF/cm²) were, respectively, PD: 1.27ª ± 0.70/2.24A ± 0.36 and FD: 1.49ª ± 0.44/2.24A ± 0.67 (p > 0.05). The mean wear values (± SD, µm) were PD: 3.63ª ± 1.54 and FD: 3.54ª ± 0.90 (p > 0.05). The mean percentSMHC values (± SD) were PD: 89.63ª ± 4.73 and FD: 87.28ª ± 4.01 (p > 0.05). Thus, we concluded that the residual fluoride from the fluoride-containing dentifrice did not protect enamel against erosion.

Efeito do ferro na composição do esmalte bovino e na placa dentária formada in situ / Effect of iron on the hardness and composition of bovine enamel and dental plaque formed in situ

O objetivo deste estudo foi avaliar, in situ, o efeito do Fe sobre a desmineralização do esmalte dentário bovino, bem como na composição da placa dentária formada sob situação de alto desafio cariogênico. Doze voluntários participaram deste estudo cruzado e cego, desenvolvido em 2 etapas de 14 dias, separadas por um intervalo de 7 dias. Em cada etapa, os voluntários receberam dispositivos palatinos contendo 4 blocos de esmalte bovino (4X4X2,5 mm). Durante o período experimental, em ambas as etapas, os voluntários realizaram escovação com dentifrício não fluoretado e oito vezes por dia, 6 voluntários gotejaram uma solução de sulfato ferroso 15 mM sobre os fragmentos, enquanto que os outros 6 gotejaram água deionizada. Cinco minutos após, uma solução de sacarose 20% fresca foi gotejada sobre todos os blocos de esmalte. A análise da dureza superficial nos blocos foi determinada antes e após a etapa in situ, utilizando um penetrador Knoop acoplado a um software com carga de 50 g por 10 seg. Ao final de cada fase experimental, a placa bacteriana formada sobre os blocos foi coletada e submetida à análise da concentração de F, P, Ca, Fe e carboidratos álcali-solúveis. Também foram avaliados a dureza em seção longitudinal (carga de 25 g por 10 seg) e a concentração de F, Ca e Fe do esmalte. Os dados foram analisados por ANOVA e teste "t" emparelhado (p < 0,05). Na placa tratada com sulfato ferroso, houve um aumento estatisticamente significante nas concentrações de P e Fe, o que não ocorreu para os teores de F, Ca e carboidratos álcali-solúveis. O grupo que recebeu o sulfato ferroso obteve % SMHC e "delta"Z significativamente menores que o grupo controle. A percentagem de volume mineral foi maior para o grupo que recebeu Fe, em todas as profundidades, embora a diferença não tenha sido estatisticamente significante para as profundidades de 110 e 220 µm. Não foi encontrada diferença significante entre os grupos com relação ao F, Ca e Fe incorporados no esmalte. Os resultados indicam que o Fe causa um aumento na concentração de P da placa dentária, bem como reduz a perda mineral, tanto em superfície, quanto em profundidade no esmalte

A utilização da técnica da mão-sobre-a-boca (MSB) em odontopediatria: revisão de literatura / The utilization of the hand-over-mouth (HOM) technique in pediatric dentistry: a review of the literature

Esta revisão de literatura tem por objetivo principal trazer à tona aspectos clínicos, éticos e legais relacionados à utilização da técnica de mão-sobre-a-boca(MSB) em Odontopediatria. As principais indicações e contra-indicações, a percepção que os profissionais, pais e/ou responsáveis pelas crianças têm desta manobra e as implicações éticas e legais deste procedimento também são discutidos