RESUMEN
<b>Objective:</b> The purpose of this study was to confirm the effect of feeding Okara fermented by <i>Bacillus coagulans</i> on cecum microflora in rat. <b>Method:</b> Three groups of SD rat of 8 weeks old were used in the experiment. The experimental animals were fed control diet, 2% okara diet, 2% fermented okara diet in each group for two weeks. The experimental diet and water were provided <i>ad libitum</i>. Food intake was measured every day. After 24 hours fast, we performed drawing blood with heparin after the experimental period. After drawing blood, several organs were removed for measuring their weight. In addition, we removed cecum after drawing blood about each three groups and studied their microflora. We also measured about plasma albumin, ALT, AST, ALP, LDH, total cholesterol, triacylglycerol, glucose, and cytokine level. <b>Result:</b> Bifidobacteria of cecum and blood IFN-g levels significantly increased in the fermented okara group. The total cholesterol level in blood significantly decreased. <b>Conclusion:</b> Okara fermented by <i>Bacillus coagulans</i> had an influence on not only intestinal microflora but also the immune system in rat, suggesting that the fermented okara can expect development as functional materials.
RESUMEN
<b>Objective:</b> The purpose of this study was to confirm the effect of Okara-puree (okara after the sub-critical water reaction) on antitumor and antipromoter activity.<br> <b>Method:</b> The antipromoter activity was investigated by colony method using JB6 Cl 41 cells and 3-3 MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. We examined the antitumor effect by measuring the survival days and the bodyweight of the mice in which colon cancer cells had been transplanted in the soles of the left foot.<br> <b>Result:</b> Okara-puree DMSO extract inhibited EGF(epidermal growth factor)-induced colonization of JB6 Cl 41 cells. In the mice transplanted with cancer cells, more survivors were seen in the Okara-puree group compared to control during the period day 10 to day 20. Furthermore,the mice in the Okara-puree group showed a longer survival time compared to control even after day 22.<br> <b>Conclusion:</b> Unlike unprocessed okara, Okara-puree shows promise for development as a functional food material with antitumor and antipromoter effects.<br>