Tumor necrosis factor-alpha gene promoter polymorphism in Iranian patients with chronic hepatitis B.

BACKGROUND: Clearance of hepatitis B virus (HBV) infection requires a good host immune response. Cytokines like tumor necrosis factor-alpha (TNF- alpha) may play a role in such immune response. Genetic changes in TNF-a gene promoter region are known to influence TNF- alpha expression. We therefore studied the role of one such mutation in chronic HBV infection. METHODS: Presence of -308 G/A polymorphism in the promoter region of TNF- alpha gene was looked for in 100 patients with chronic HBV infection, 91 subjects with spontaneously recovered HBV infection and 89 healthy controls, using a PCR-RFLP method. RESULTS: Variant alleles (A/A or A/G) were found in 22 of 100 (22%) patients with chronic HBV infection, 21 of 91 (23%) subjects with spontaneous HBV clearance and 14 of 89 (15.7%) control subjects (p=ns for inter-group comparisons). CONCLUSION: TNF- alpha promoter polymorphism -308A is common in Iranian population, but has no association with development of chronic HBV infection.

Peak Expiratory Flow Rates Among Gujjar and Non-Gujjar Population of Kashmir Valley

Peak expiratory flow rates of 506 healthy adults from a rural area of Kashmir consisting of 252 Gujjars and 254 non-Gujjars are reported. There is paucity of literature an ventilatory measurements of such populations living at high attitudes and this study is first of this kind from Kashmir valley. Gujjars were found to have lower peak expiratory flow rates than non-Gujjars. Role of domestic smoke pollution (measured as time spent near fire place) and smoking in lowering the peak expiratory flow rates has also been documented.