RESUMEN
It has been documented that cytokines play important roles in the induction of normal functions of the placenta. It has been hypothesized that abnormal expression of the cytokines may be associated with unsuccessful pregnancy. The aim of this study was to compare the serum levels of interleukin- 17A [IL-17A] and tumor growth factor [TGF-beta] in pre-term, term neonates, and their corresponding mothers. This study was performed on 100 term and 60 pre-term neonates, and also on their corresponded mothers. Serum levels of IL-17A and TGF-beta were examined by enzyme linked immunosorbent assay [ELISA]. Our results revealed that the serum levels of IL-17A were significantly decreased in pre-term neonates in comparison to full-term neonates. However, the serum levels of IL-17A in the mothers either with pre-term or full-term neonates were not different. Also the serum levels of TGF-beta were not changed in pre-term neonates and their mothers when compared with full-term neonates and their mothers, respectively. Based on these findings, it can be concluded that IL-17A may play crucial roles in induction of normal pregnancies and also probably participates in normal growth of fetus
RESUMEN
Background and Aim: Ferula gummosa Boiss. [Barije] is an Iranian indigenous plant that has medical and antifungal properties. The current study was done to determine the effect of aqueous an alcoholic extracts of Ferula gummosa Boiss root on in vitro growth of fungi
Materials and Methods: In this experimental study, the plant was dried in the dark and aqueous, alcoholic extracts of its root powder were prepared using Soxhlet method. Then, the efficacy of 0.1 dilutions of different quantities of the extracts on strains of Candida albicans [PTCC 5027], Trichophyton rubrum [PTCC5143], and Aspergillus fumigatus [PTCC 5009] were evaluated employing Disk diffusion, Agar-well diffusion, Minimum Inhibitory Concentration [MIC], and Minimum Fungicidal Concentration [MFC] methods
Results: No inhibited effect was observed in Disk diffusion and Agar-well diffusion methods. MIC and MFC values of all extracts for Aspergillus fumigatus and Trichophyton rubrum were more than 5×104mg/ml. The MIC values of ethanol and methanol extract for Candida albicans were 3.12×103 and 1.25×104mg/ml, respectively but the MFC values were 1.25×104 and 2.5×104mg/ml, respectively
Conclusion: Methanol and ethanol extracts proved to have antifungal activity against Candida albicans yeast in vitro while the fungi of Aspergillus fumigatus and Trichophyton rubrum had no sensitivity to these types of extracts
RESUMEN
Introduction and Aims: proteinuria means the presence of an excess of serum proteins in the urine that is very important to achieve accurate and reliable results to the physicians. The aim of this study was to evaluate the interference of turbidity in the measurement of 24-hour urine protein before and after cold treatment in turbid centrifuged samples
Materials and Methods: this experimental study was performed in ACECR [Academic Center for Education, Culture and Research], that was conducted on a random sample of 1000 people in 2015 March to September. The measurement of 24-hour urine protein was performed by using three chloral acetic acid method [TCA] and photometer
Results: ten samples [1%] just were turbid after centrifuge. There was a significant difference [P<0.05] between the samples which were turbid after centrifuge and the samples which their turbidity have removed by cold treatment. So that, in some cases the interpretation of the results after cold treatment is quite different with before [the lowest difference was 0.05 and most difference was 0.43]
Conclusion: in order to deposit interfering factors, the urine samples that are taken to measure protein and are turbid after centrifuge should be given cold treatment and centrifuge again