RESUMEN
A study was carried out to investigate the effects of Naphthalene Acetic Acid (NAA) and sugars on the plants and flowers quality of Mokara Chark Kuan orchid respectively. Parameters were monitored between November 2014 to March 2015, with the application of three concentrations of NAA at 25, 50 and 100 mg/L in the field. The application of 25 mg/L NAA significantly increases the plant height, number of roots and total soluble solid content of Mokara Chark Kuan orchid plants. But, there was no significant effect on the leaves number, chlorophyll content and number of flowers. In case of storage experiment, the cut Mokara Chark Kuan flowers were treated with 3 % and 6 % glucose and sucrose. Physiological parameters: fresh weight, water uptake, petal thickness, discoloration and vase life were evaluated. It can be concluded that spraying with 25 mg/L NAA enhanced the plant growth and development of Mokara Chark Kuan orchid. The study also showed that 6 % sucrose was the best treatment for maintaining the post-harvest quality as well as vase life of cut Mokara Chark Kuan orchid flowers.
Um estudo foi realizado para investigar os efeitos do ácido naftaleno ácido acético (NAA) no crescimento de e açúcares no plantas e na qualidade de flores da orquídea Mokara Chark Kuan . Os parâmetros foram monitorados entre novembro de 2014 e março 2015, com a aplicação de três concentrações de ANA em 25, 50 e 100 mg / L sob condições de campo. A aplicação de 25 mg / L de ANA aumentou significativamente a altura da planta, o número de raízes e o teor de sólidos solúveis totais de plantas de orquídeas Mokara Chark Kuan. Todavia, não houve nenhum efeito significativo sobre o número de folhas, o teor de clorofila e o número de flores. No caso do experimento de armazenamento, flores cortadas de Mokara Chark Kuan foram tratadas com 3% e 6% de glicose e de sacarose. Os parâmetros fisiológicos: peso fresco, absorção de água, espessura pétala, descoloração e vida de vaso foram avaliados. Pode concluir-se que a pulverização com 25 mg / L de NAA aumentou o crescimento e o desenvolvimento das plantas de orquídea Mokara Chark Kuan. O estudo também mostrou que 6% de sacarose foi o melhor tratamento para a manutenção da qualidade pós-colheita, bem como vida de vaso em flores de corte da orquídea Mokara Chark Kuan .
Asunto(s)
Sacarosa , Orchidaceae/crecimiento & desarrollo , GlucosaRESUMEN
A nondestructive, efficient, and accurate fingerprinting method using Fourier transform infrared spectroscopy (FTIR) has been developed and optimized for the investigation and demonstration of the variance in chemical characteristics among extracts of Ficus deltoidea Jack var. bornensis from different but closely situated origins. The capacity of attenuated total reflectance (ATR) to differentiate these samples were studied using methanol and water extracts which were preliminarily screened using HPTLC with vitexin and isovitexin being used as markers for authentication. The mobile phase used was ethyl acetate: formic acid (0.1%): methanol at ratio of 5:5:2 (v/v/v) and the profile showed that methanol extracts had higher affinity for the markers. The FTIR spectra indicated that there was no obvious difference in spectroscopic pattern for either extracts when comparing samples from different localities but the absorption intensities of some peaks were different. Multivariate statistical analyses of PCA and HCA showed that both these techniques were capable of identifying the most similar as well as most differing samples and the identification depended on the type of extract. Overall, FTIR fingerprinting has the potential to be a fast and reliable analytical methodology for the discrimination between variants of plant from closely situated locations and hence chemically similar samples.