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3.
Braz. j. med. biol. res ; 29(7): 905-9, July 1996.
Artículo en Inglés | LILACS | ID: lil-181500

RESUMEN

The expression of the cellulase transcripts of Trichoderma reesei is controlled by the nature of the energy carbon sources used in the culture medium. Cellulose and the soluble disaccharide sophorose, but not glycerol or glucose, act as inducers. Evidence is presented suggesting that a low constitutive extracellular cellulolytic system catalyzes the formation of a soluble inducer from cellulose, and this inducer triggers the expression of the cellulase transcripts. This basal and cellulose-induced expression of the cellobiohydrolase I mRNAs (cbh1), the major member of the cellulase system, is transcriptionally controlled by two independent cis-acting DNA regions. In addition, expression of the cbh1 transcript is influenced by the physiological state of the mitochondria and this sensitivity is controlled through the 5,-flanking DNA sequence of this gene.


Asunto(s)
Celulasa/genética , Transcripción Genética , Trichoderma/genética , Carbono , Celulasa/metabolismo , Celulosa/farmacología , Mitocondrias/metabolismo , Transcripción Genética , Trichoderma/metabolismo
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