Rank, rankl, and opg in dentigerous cyst, odontogenic keratocyst, and ameloblastoma: a meta-analysis

Abstract The aim of this study was to assess and compare RANK, RANKL, and OPG immunoexpression in dentigerous cyst, odontogenic keratocyst, and ameloblastoma. The protocol was registered in PROSPERO (CRD42018105543). Seven databases (Embase, Lilacs, LIVIVO, PubMed, Scopus, SciELO, and Web of Science) were the primary search sources and two databases (Open Grey and Open Thesis) partially captured the "grey literature". Only cross sectional studies were included. The JBI Checklist assessed the risk of bias. A meta-analysis with random effects model estimated the values from the OPG and RANKL ratio reported by the individual studies and respective 95% confidence intervals. The heterogeneity among studies was assessed with I2 statistics. Only nine studies met the inclusion criteria and were considered in the analyses. The studies were published from 2008 to 2018. Two studies presented low risk of bias, while seven studies presented moderate risk. The meta-analysis showed the highest OPG>RANKL ratio for dentigerous cyst (ES=43.3%; 95% CI=14.3-74.8) and odontogenic keratocyst (ES=36.8%; 95% CI=18.8-56.7). In contrast, the highest OPG<RANKL ratio was found for ameloblastoma (ES=73.4%; 95% CI=55.4-88.4) and it was higher in the stromal region compared to the odontogenic epithelial region. The results may explain the aggressive potential of ameloblastoma from the higher OPG<RANKL ratio in this tumor, while it was lower for dentigerous cyst and odontogenic keratocyst.

Resumo O objetivo deste estudo foi avaliar e comparar a imunoexpressão de RANK, RANKL e OPG em cisto dentígero, ceratocisto odontogênico e ameloblastoma. O protocolo foi registrado no PROSPERO (CRD [Oculto]). Sete bancos de dados (Embase, Lilacs, LIVIVO, PubMed, Scopus, SciELO e Web of Science) foram as principais fontes de pesquisa e duas bases de dados (Open Grey e Open Thesis) capturaram parcialmente a "literatura cinza". Apenas estudos transversais foram incluídos. A ferramenta JBI avaliou o risco de viés. Uma metanálise com modelo de efeitos aleatórios estimou os valores da razão OPG e RANKL relatados pelos estudos individuais e seus respectivos intervalos de confiança de 95%. A heterogeneidade entre os estudos foi avaliada por meio do teste I2. Apenas nove estudos preencheram os critérios de inclusão e foram considerados nas análises. Os estudos foram publicados entre 2008 e 2018. Dois estudos apresentaram baixo risco de viés, enquanto sete estudos apresentaram risco moderado. A meta-análise mostrou a maior razão OPG> RANKL para cisto dentígero (ES=43,3%; IC95%=14,3-74,8) e ceratocisto odontogênico (ES=36,8%; IC95%=18,8-56,7). Por outro lado, a maior razão OPG <RANKL foi encontrada para ameloblastoma (ES=73,4%; IC95%=55,4-88,4) e foi maior na região estromal em comparação com a região epitelial odontogênica. Os resultados podem explicar o potencial agressivo do ameloblastoma devido a uma maior proporção OPG <RANKL nesse tumor, enquanto tal proporção foi menor no cisto dentígero e no ceratocisto odontogênico.

Accuracy of the dental pulp sensibility test using cold spray for the diagnosis of pulp diseases: an observational clinical study

The dental pulp sensibility test is one of the main auxiliary resources for the diagnosis of pulp pathologies, and its accuracy is still debatable. This cross-sectional observational study evaluated the accuracy of the pulp sensibility test (PST) using cold spray (1,1,1,2-tetrafluoroethane) for the diagnosis of pulp diseases and determined the effect of individual and clinical variables on the reliability of this test. The paper was designed following the STROBE statement. Sixty patients with indications for primary endodontic treatment were selected and examined from August 2017 to July 2018. Data collection was performed through interviews, clinical/radiographic examinations and the PST. The results of the cold test, along with data on sex, age, the tooth type regarding the root number, and the presence of restorations and caries, as well as the recent consumption of analgesics, were recorded. The presence of bleeding within the pulp chamber was used as the gold standard to compare with the clinical diagnosis and to identify the true-positive, false-positive, true-negative, and false-negative responses. The accuracy of PST achieved in subgroups of individual and clinical variables was compared using the chi-square test with a significance level of 5% (p < 0.05). The PST with the use of cold spray showed a sensitivity of 0.88, a specificity of 1.00, a positive predictive value of 1.00, a negative predictive value of 0.86, and an accuracy of 0.93. The accuracy of the cold spray was not affected by individual or clinical variables. The PST with the use of cold spray is an accurate and reliable method for determining the diagnosis of pulp diseases, especially in cases of pulp vitality or irreversible pulpitis.

Toxicity and effect of whitening toothpastes on enamel surface

Abstract This in vitro study evaluated the biocompatibility and abrasivity of whitening and conventional toothpastes. Samples of conventional (non-whitening) - Edel White Infant (EWI) - and whitening toothpastes - Edel White Whitening (EWW), Edel White CAREFORTE (EWC), Colgate Total 12 Ò Professional (C), and Oral-B Whitening (OB) - were dissolved in culture medium (0.2 g sample weight per mL). Human gingival fibroblasts (hGF) were placed in contact with different dilutions of culture media that had been previously exposed to these toothpastes. Cytotoxicity was then assessed using the methyl tetrazolium test (MTT) and the cell survival rate was determined. Genotoxicity was assessed by the micronucleus test (MNT) and the number of micronuclei was determined before and after exposure to the toothpaste solutions. The enamel surface roughness was evaluated in specimens of bovine teeth (n = 10 per group) before and after 10,000 brushing cycles, using the investigated toothpastes. The results were statistically analyzed using the Mann-Whitney U test and two-way ANOVA (p < 0.05). According to the MTT assay, EWW and OB presented significant cytotoxicity (p < 0.01), but no genotoxic (MNT) effects (p > 0.05). C toothpaste was statistically significantly abrasive to the enamel surface (p < 0.01). The findings of this study may be helpful for individualized selection of commercial toothpastes, as some whitening toothpastes present significant cytotoxicity and conventional toothpaste cause significant surface changes.

Polymorphisms of matrix metalloproteinase-7 and -9 are associated with oral tongue squamous cell carcinoma

Abstract Matrix degradation is an important event in the progression, invasion and metastasis of malignant head and neck lesions. Imbalances, mutations and polymorphisms of MMPs and their inhibitors are observed in several cancer subtypes. The aim of this study was to evaluate the association of the MMP-7 gene promoter (181 A/G) and MMP-9 (-1562 C/T) polymorphisms in oral tongue squamous cell carcinoma (OTSCC). MMP-7 (rs11568818) and MMP-9 (rs3918242) single-nucleotide polymorphisms (SNPs) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 71 cases of OTSCC. Normal tissue specimens were obtained from 60 healthy volunteers to serve as the control. The MMP-7 G allele and MMP-9 T allele were more frequent in the OTSCC group than the control group, but only when these two SNPs were taken together was a significant association found with the nodal metastasis of OTSCC (p < 0.001). Based on our results, SNPs in the promoter region of MMP-7 and MMP-9 appear to be associated with greater risk of developing OTSCC, and with a higher propensity to form metastatic tumors. In this respect, molecular studies investigating polymorphisms may be useful in predicting tumor behavior.

Assessment of carvacrol paste as endodontic medication associated with laser photobiomodulation in tissue repair: an experimental study in rats / Avaliação da pasta de carvacrol como medicação endodôntica associada à fotobiomodulação a laser no reparo: um estudo experimental em ratos

This study aimed to analyze the tissue reaction caused by carvacrol paste associated or not with laser photobiomodulation (LPBM) at λ660 nm in the subcutaneous tissue of rats. Sixty Wistar rats were divided into four groups and they received the following interventions: subcutaneous implantation of empty polyethylene tubes (CTR), implantation of tubes containing carvacrol paste (CVC), implantation of empty tubes and LPBM (LLLT), and implantation of tubes containing carvacrol paste and LPBM (CVCLT). The animals were euthanized at three, eight, and 15 days after surgery. The inflammatory reaction and fibroplasia were analyzed histomorphometrically. Significant differences among the groups were determined by ANOVA and Tukey's test (p<0.05). In the 3-day period, the CVCLT group had low inflammatory infiltration (p<0.01). In the 8- and 15-day periods, the LLLT and CVCLT groups presented a low amount of lymphocytic inflammatory infiltrate (p<0.01 and p<0.05). Regarding the formation of fibrous tissue, the CVC group had the highest formation of type III collagen in the 8-day period (p<0.001). In the 15-day period, the CVCLT group had a lower formation of type I collagen than the CTR and LLLT groups (p<0.05). The use of the carvacrol paste associated with photobiomodulation optimizes the inflammatory period and tissue repair.

Este estudo teve como objetivo analisar a reação tecidual causada pela pasta de carvacrol associada ou não à fotobiomodulação a laser (LPBM) a λ660 nm no tecido subcutâneo de ratos. Sessenta ratos Wistar foram divididos em quatro grupos e receberam as seguintes intervenções: implantação subcutânea de tubos de polietileno vazios (CTR); implantação de tubos contendo pasta de carvacrol (CVC); implantação de tubos vazios e LPBM (LLLT); implantação de tubos contendo pasta de carvacrol e LPBM (CVCLT). Os animais foram eutanasiados aos 03, 08 e 15 dias após a cirurgia. A reação inflamatória e a fibroplasia foram analisadas histologicamente. Diferenças significativas entre os grupos foram determinadas pelo teste ANOVA e teste de Tukey (p<0,05). No período de três dias, o grupo CVCLT apresentou menor infiltração inflamatória (p<0,01). No período de 8 e 15 dias, os grupos LLLT e CVCLT apresentaram menor quantidade de infiltrado inflamatório linfocitário (p<0,01 e p<0,05). Em relação à formação de tecido fibroso, o grupo CVC apresentou maior formação de colágeno tipo III no período de 8 dias (p<0,001). No período de 15 dias, o grupo CVCLT apresentou menor formação de colágeno tipo I em relação aos grupos CTR e LLLT (p<0,05). O uso da pasta de carvacrol associado à fotobiomodulação a laser otimiza o período inflamatório e o reparo tecidual.

Deglutition manifestations in patients with oropharyngeal cancer subjected to conservative therapy: systematic review / Manifestações da deglutição em pacientes com câncer de orofaringe submetidos à terapia conservadora: revisão sistemática

ABSTRACT Objectives: To characterize scientific production and identify deglutition changes in individuals with oropharyngeal cancer subjected to conservative therapy. Methods: The search was applied to five electronic database [Scientific Electronic Library Online (Scielo), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), US National Library of Medicine National Institutes of Health (PubMed), Web of Science, and Scopus], besides the search of grey literature in the databases (OpenThesis e OpenGrey), avoiding selection and publication bias. Prospective longitudinal studies concerning the theme: deglutition disorders in individuals with oropharyngeal cancer subjected to conservative therapy were considered eligible. The risk of bias and the evaluation of individual methodological quality of the selected studies were measured by "The Joanna Briggs Institute Critical Appraisal tools for use in JBI Systematic Reviews" for prospective and longitudinal studies. Results: The search resulted in 899 records and after analysis four of them fulfilled the eligibility criteria. Among the studies included, all individuals presented some type of deglutition changes, the most common were: reduced of strength and retraction of the base of the tongue, delayed deglutition trigger, reduced laryngeal elevation, presence of residues on tongue and palate, in the pharyngeal area, valleculae, and posterior pharyngeal wall, as well as in the vestibules and in pyriform sinuses. Conclusion: The evidence from this systematic review suggests that conservative therapies cause deglutition changes or amplify the pre-existing ones, regardless of the type and magnitude of radiation, as well as tumor staging. However, there is little standardization in the research methodologies, making a meta-analysis study difficult to conduct.

RESUMEN Objetivos: Caracterizar la producción científica e identificar las alteraciones de deglución en personas con cáncer de orofaringe sometidas a tratamiento conservador. Métodos: Se realizó una búsqueda en cinco bases de datos electrónicas [Scientific Electronic Library Online (Scielo), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), US National Library of Medicine National Institutes of Health (PubMed), Web of Science y Scopus], además de la literatura gris en las bases de datos OpenThesis y OpenGrey, evitando sesgo de selección y publicación. Se consideraron elegibles los estudios longitudinales prospectivos sobre el tema: trastornos de deglución en pacientes con cáncer de orofaringe sometidos a tratamiento conservador. El riesgo de sesgo y la calidad metodológica individual de los estudios seleccionados se evaluaron mediante la herramienta de evaluación crítica del Instituto Joanna Briggs (JBI) para uso de sus revisiones sistemáticas, estudios prospectivos y longitudinales. Resultados: La búsqueda encontró 899 registros y, tras análisis, cuatro de ellos cumplieron los criterios de elegibilidad. Entre los estudios incluidos, todos los pacientes presentaron algún tipo de trastorno de la deglución. Los más frecuentes fueron: fuerza y retracción reducidas de la base de la lengua, retraso en el disparo de la deglución, elevación laríngea reducida, presencia de residuo en lengua y paladar, en región faríngea, valléculas y pared posterior de la faringe, así como en el interior de los vestíbulos y en los senos piriformes. Conclusión: Esta revisión sistemática sugiere que los tratamientos conservadores producen alteraciones de deglución o intensifican aquellas que ya existen, independientemente del tipo y de la intensidad de radiación, así como de la estadificación del tumor. Hay, sin embargo, poca estandarización en las metodologías de investigación, lo que hace difícil un estudio de metanálisis.

RESUMO Objetivos: Caracterizar a produção científica e identificar as alterações da deglutição em indivíduos com câncer de orofaringe submetidos à terapia conservadora. Métodos: Realizou-se uma busca em cinco base de dados eletrônicas [Scientific Electronic Library Online (Scielo), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), US National Library of Medicine National Institutes of Health (PubMed), Web of Science e Scopus], além da busca da literatura cinzenta nas bases de dados (OpenThesis e OpenGrey), evitando viés de seleção e publicação. Foram considerados elegíveis estudos longitudinais prospectivos sobre o tema: alterações de deglutição em indivíduos com câncer de orofaringe submetidos à terapia conservadora. O risco de viés e a qualidade metodológica individual dos estudos selecionados foram avaliados pela ferramenta de avaliação crítica do Joanna Briggs Institute (JBI) para uso de suas revisões sistemáticas, estudos prospectivos e longitudinais. Resultados: A busca resultou em 899 registros e, após análise, quatro deles atenderam aos critérios de elegibilidade. Entre os estudos incluídos, todos os indivíduos apresentaram algum tipo de alteração de deglutição; os mais frequentes foram: força e retração da base da língua reduzidas, atraso no disparo da deglutição, elevação laríngea reduzida, presença de resíduo em língua e palato, em região faríngea, valéculas e parede posterior da faringe, bem como no interior dos vestíbulos e em seios piriformes. Conclusão: Esta revisão sistemática sugere que as terapias conservadoras produzem distúrbios de deglutição ou intensificam os já existentes, independentemente do tipo e da intensidade de radiação, bem como do estadiamento do tumor. Há, no entanto, pouca padronização nas metodologias das pesquisas, dificultando um estudo de metanálise.

Clinical performance of whitening on devitalized teeth: a retrospective observational study / Desempenho clínico do clareamento em dentes desvitalizados: estudo observacional retrospectivo

Objective: This retrospective observational study evaluated the clinical performance of intracoronal whitening and correlated the main factors that interfere with its stability and patient satisfaction. Material and Methods: The paper was designed following the STROBE statement. Patients from the Institute of Science and Technology - ICT/UNESP database who underwent nonvital tooth whitening from August 2010 to July 2015 were selected. Data collection was performed by researching the institution records, patients interview, clinical and radiographic examination. Data were analyzed using MannWhitney test and Spearman's correlation (p < 0.05). Results: The initial search on 1275 records resulted in 43 patients, of whom 18 were selected according to eligibility criteria. There was positive correlation only between the degree of patient's satisfaction and the perception of color difference after whitening treatment (p < 0.05). Color relapse was observed in all conditions, independently of the period of followup (p > 0.05). There was no case of external cervical root resorption. Conclusion: Color changes after whitening influenced patient's satisfaction. There was no correlation among the color relapse and height of gutta-percha, return interval or cause of darkening (AU)

Objetivo: Este estudo observacional retrospectivo avaliou o desempenho clínico do clareamento interno e correlacionou os principais fatores que interferem em sua estabilidade e satisfação do paciente. Material e Métodos: O artigo foi elaborado seguindo o STROBE. Pacientes do banco de dados do Instituto de Ciência e Tecnologia ­ ICT/UNESP que foram submetidos ao clareamento dental em dentes desvitalizados entre agosto de 2010 e julho de 2015 foram selecionados. A coleta de dados foi realizada por meio de pesquisa em prontuários da instituição, entrevista com os pacientes, exames clínico e radiográfico. Os dados foram analisados usando o teste de Mann-Whitney e correlação de Spearman (p < 0,05). Resultados: A pesquisa inicial em 1275 registros resultou em 43 pacientes, dos quais 18 foram selecionados de acordo com os critérios de elegibilidade. Houve correlação positiva apenas entre o grau de satisfação do paciente e a percepção da diferença de cor após o tratamento clareador (p < 0,05). A recidiva da cor foi observada em todas as condições, independentemente do tempo de acompanhamento (p > 0,05). Não houve nenhum caso de reabsorção cervical externa. Conclusão: As mudanças de cor após o clareamento influenciaram a satisfação do paciente. Não houve correlação entre a recidiva da cor e a altura do guta-percha, o intervalo de retorno ou a causa do escurecimento. (AU)

Influence of interleukins on prognosis of patients with oral squamous cells carcinoma / Influência de interleucinas no prognóstico de pacientes portadores de carcinoma de células escamosas intraoral

ABSTRACT Squamous cell carcinoma is a malignant neoplasm that affects the structures and tissues of the oral cavity. Interleukins (IL) synthesized from a tumor modulate a cascade reaction that may influence the prognosis of the disease. We aim to investigate in the literature whether interleukins are mediators that negatively or positively influence the prognosis of patients with oral squamous cell carcinoma. A systematic review study was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes (PRISMA) instructions. PubMed (including MedLine), Scopus, Web of Science, SciELO and Latin American and Caribbean Health Sciences (LILACS) databases were used as the primary sources for the study; OpenGrey and OpenThesis were used to search for "gray literature". The search conducted in seven general databases resulted in a set of 858 studies, while the search conducted in two databases for gray literature resulted in 82 studies, totaling 940 studies. From these, 15 studies were selected for this systematic review (eight studies presented low bias risk; four studies presented moderate bias risk; and three studies presented high risk of bias). Although they may act on the anti-tumor immune response pathways, the IL evaluated in the present systematic review (IL-4, IL-6, IL-8, IL-10, IL-12 and IL-13) tend to present a response associated with the intensification of carcinogenesis and poor prognosis in patients with oral squamous cell carcinoma.

RESUMEN El carcinoma de células escamosas es una neoplasia maligna que afecta las estructuras y los tejidos de la cavidad oral. Interleucinas (IL) sintetizadas a partir de un tumor modulan una cascada de reacciones que puede influenciar el pronóstico de la enfermedad. Nuestro objetivo es investigar en la literatura si las IL son mediadoras que influyen negativamente o positivamente en el pronóstico de pacientes con carcinoma oral de células escamosas. Un estudio de revisión sistemática fue realizado bajo las instrucciones del Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Las bases de datos PubMed (incluyendo MedLine), Scopus, Web of Science, SciELO y Latin American and Caribbean Health Sciences (LILACS) fueron usadas como fuentes primarias de estudio; OpenGrey y OpenThesis, para pesquisar la literatura gris. La búsqueda realizada en siete bases de datos generales resultó en un conjunto de 858 estudios, mientras la búsqueda realizada en dos bases de datos para literatura gris resultó en 82 estudios, totalizando 940 investigaciones. Entre ellas, 15 fueron elegidas para la presente revisión sistemática (ocho estudios presentaron bajo riesgo de sesgo; cuatro, riesgo moderado; tres, riesgo alto). A pesar de poder actuar en vías de respuesta inmune antitumoral, las IL evaluadas en esta revisión (IL-4, IL-6, IL-8, IL-10, IL-12, y IL-13) suelen presentar una respuesta asociada a la intensificación del proceso de carcinogénesis y al pronóstico desfavorable en pacientes portadores de carcinoma oral de células escamosas.

RESUMO O carcinoma de células escamosas é uma neoplasia maligna que afeta as estruturas e os tecidos da cavidade oral. Interleucinas (IL) sintetizadas a partir de um tumor modulam uma cascata de reações que poderá influenciar o prognóstico da doença. Objetiva-se investigar na literatura se as interleucinas são mediadores que influenciam negativamente ou positivamente o prognóstico de pacientes com carcinoma de células escamosas intraoral. Um estudo de revisão sistemática foi realizado segundo as instruções do Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). As bases de dados PubMed (incluindo MedLine), Scopus, Web of Science, SciELO e Latin American and Caribbean Health Sciences (LILACS) foram utilizadas como fontes de estudo primárias; OpenGrey e OpenThesis, utilizadas para pesquisar a "literatura cinzenta". A busca realizada em sete bases de dados gerais resultou em um conjunto de 858 estudos, enquanto a realizada em duas bases de dados para literatura cinza, em 82 estudos, totalizando 940 pesquisas. Destas, 15 foram selecionadas para a presente revisão sistemática (oito estudos apresentaram baixo risco de viés; quatro, moderado risco de viés; e três, alto risco de viés). Apesar de poderem atuar nas vias de resposta imune antitumoral, as IL avaliadas nesta revisão (IL-4, IL-6, IL-8, IL-10, IL-12 e IL-13) tendem a apresentar uma resposta associada à intensificação do processo de carcinogênese e ao prognóstico desfavorável em pacientes portadores do carcinoma de células escamosas intraoral.

Microbial profile and endotoxin levels in primary periodontal lesions with secondary endodontic involvement

Abstract This study was carried out to investigate the microbial profile and endotoxin levels of endodontic-periodontal lesions of periodontal origin. Periodontal and endodontic samples were taken from periodontal pockets and necrotic root canals of 10 teeth with endodontic-periodontal lesions. Evidencing of 40 different bacterial species were determined in each endodontic and periodontal sample using the checkerboard DNA-DNA hybridization method and Kinetic chromogenic LAL assay was used for quantification of endotoxins. Fisher's exact test correlated the bacterial species with the endodontic or periodontal microbiota. The endotoxin levels (EU/mL) found in samples of the root canal and periodontal pocket were compared by the Wilcoxon test (p<0.05). Bacteria and LPS units were found in 100% of the endodontic and periodontal samples. The species E. faecium, P. acnes, G. morbillorum, C. sputigena and L. buccalis were strongly correlated with the endodontic microbiota and P. nigrescens with the periodontal microbiota. P. intermedia, P. endodontalis and V. parvula were more prevalent in both endodontic and periodontal microbiots. The endotoxin levels in the periodontal pocket (89600 EU/mL) were significantly higher than in the root canal (2310 EU/mL). It was concluded that the microbiota present in the periodontal and endodontic tissues is similar, with a higher prevalence of species of the orange complex and a higher level of endotoxin in the periodontal pockets.

Resumo Este estudo foi realizado para investigar o perfil microbiano e os níveis de endotoxina de lesões endoperiodontais de origem periodontal. Amostras periodontais e endodônticas foram obtidas de bolsas periodontais e canais radiculares necróticos de 10 dentes com lesões endoperiodontais. A investigação de 40 espécies bacterianas diferentes foram determinadas em cada amostra endodôntica e periodontal usando o método de hibridização de DNA-DNA (checkerboard) e o ensaio cinético cromogênico LAL foi usado para quantificação de endotoxinas. O teste exato de Fisher correlacionou as espécies bacterianas com a microbiota endodôntica ou periodontal. Os níveis de endotoxina (EU/mL) encontrados nas amostras do canal radicular e na bolsa periodontal foram comparados pelo teste de Wilcoxon (p<0,05). Bactérias e unidades de LPS foram encontradas em 100% das amostras endodônticas e periodontais. As espécies E. faecium, P. acnes, G. morbillorum, C. sputigena e L. buccalis foram fortemente correlacionadas com a microbiota endodôntica e P. nigrescens com a microbiota periodontal. P. intermedia, P. endodontalis e V. parvula foram mais prevalentes em ambas microbiotas endodôntica e periodontal. Os níveis de endotoxina na bolsa periodontal (89600 EU/mL) foram significativamente maiores do que no canal radicular (2310 EU/mL). Concluiu-se que a microbiota presente nos tecidos periodontal e endodôntico é semelhante, com maior prevalência de espécies do complexo laranja e maior nível de endotoxina nas bolsas periodontais.

Lateralização do nervo alveolar inferior em mandíbula atrófica / Lateralization of the lower alveolar nerve in atrophic mandible

A atrofia óssea fisiológica decorrente da perda dentária associada a doenças sistêmicas como osteoporose limita a reabilitação oral. Pacientes acometidos por estes defeitos e que utilizam por longo prazo bifosfonatos possuem risco aumentado de osteonecrose. São debilitados funcionalmente, aparentam fragilidade, podendo elevar o índice de fratura patológica da mandíbula. Nesse sentido, os sistemas de fixação com placas mais rígidas são indicadas como opção terapêutica capaz de suportar as forças biomecânicas. Aliada a esta, a lateralização do nervo alveolar inferior poderá ser efetuada para evitar dano ao mesmo quando forem colocadas as placas e os parafusos de fixação. O objetivo deste trabalho foi descrever um caso clinico de tratamento de lesão de grande extensão em mandíbula atrófica decorrente da osteonecrose induzida por uso de bifosfonatos com placa de reconstrução pré-modelada com recurso de prototipagem e lateralização do nervo alveolar por meio de acesso intraoral. Conclui-se que, a técnica de lateralização do nervo alveolar inferior permitiu a utilização da placa de reconstrução, sendo considerado um método terapêutico com baixo índice de morbidade, sem evidência de parestesia após quatro meses, repercutindo de forma positiva na qualidade de vida do paciente(AU)

Physiological bone atrophy due to dental loss associated with systemic diseases such as osteoporosis limits oral rehabilitation. Patients affected by these defects and who use long-term bisphosphonates have an increased risk of osteonecrosis. They are functionally weakened, appear brittle, and may elevate the index of pathological fracture of the mandible. In this sense, the fixation systems with more rigid plates are indicated as a therapeutic option capable of withstanding the biomechanical forces. Allied to this, the lateralization of the inferior alveolar nerve can be effected to avoid damage to it when the plates and the fixing screws are placed. The objective of this work was to describe a clinical case of treatment of a large lesion in the atrophic mandible due to the osteonecrosis induced by the use of bisphosphonates with a pre-shaped reconstruction plate with prototyping and lateralization of the alveolar nerve by means of intraoral access. It was concluded that the technique of lateralization of the inferior alveolar nerve allowed the use of the reconstruction plate, being considered a therapeutic method with low morbidity index, without evidence of paresthesia after four months, positively affecting the quality of life of the patient(AU)

Effect of two erosive protocols using acidic beverages on the shear bond strength of orthodontic brackets to bovine enamel

Abstract Objective: To assess the short-term effect of two in vitro erosive challenge protocols on the bond strength of metal orthodontic brackets on bovine enamel. Methods: Sixty bovine incisors were selected and randomly divided into six groups: AS7 (artificial saliva - 7 days, Control Group); CC7 (Coca-Cola™ - 7 days); LJ7 (lime juice - 7 days); AS30 (artificial saliva - 30 days, Control Group); CC30 (Coca-Cola™ - 30 days); LJ30 (lime juice - 30 days). Microhardness testing was performed prior to the erosive challenge to verify the standardization of samples. Immersion was performed 4x/day for five minutes, for either 7 or 30 days. After immersions were concluded, the brackets were bonded and shear bond strength was assessed after 48 hours. The Adhesive Remnant Index (ARI) was also assessed. Data were analyzed by two-way ANOVA, followed by Tukey's post-hoc and Student's t test for paired samples, and the Kruskal-Wallis non-parametric test (α = 5%). Results: The mean and standard deviation of microhardness testing of total samples were 281.89 ± 44.51 KHN. There was no statistically significant difference in shear bond strength for the time factor (7 or 30 days; F5.54= 0.105; p = 0.901). However, there was a statistically significant difference for the solution factor (F5.54= 6.671; p = 0.003). These differences occurred among solutions of Saliva x Coca-Cola™ (p = 0.003) and Coca-Cola™ x Lime Juice (p= 0.029). The assessment of the Adhesive Remnant Index showed no significant difference between groups. Conclusions: The immersion time used in the erosion protocols did not affect the bond strength of brackets to teeth. Coca-Cola™ induced significantly higher shear bond strength values than lime juice and artificial saliva. However, the short term effects of 7/30 days in this in vitro study may not be extrapolated for in vivo ones. Clinical studies should be conducted, substantiating the laboratory results.

Resumo Objetivo: avaliar o efeito de curto prazo de dois protocolos de desafio erosivo, in vitro, na resistência adesiva de braquetes ortodônticos metálicos em esmalte bovino. Métodos: Sessenta incisivos bovinos foram selecionados e divididos aleatoriamente em seis grupos: SA7 (saliva artificial - 7 dias, Grupo Controle); CC7 (Coca-Cola® - 7 dias); SL7 (suco de limão - 7 dias); SA30 (saliva artificial - 30 dias, Grupo Controle); CC30 (Coca-Cola® - 30 dias); SL30 (suco de limão - 30 dias). Foi realizado o teste de microdureza antes do desafio erosivo, para verificar a padronização das amostras. A imersão foi realizada quatro vezes ao dia, por cinco minutos, durante 7 ou 30 dias. Finalizadas as imersões, os braquetes foram colados e, após 48 horas, foi avaliada a resistência ao cisalhamento. O Índice de Adesivo Remanescente (IAR) também foi avaliado. Para análise dos dados, foram utilizados os testes ANOVA dois fatores, seguido do post-hoc de Tukey e teste t de Student para amostras pareadas, e o teste não-paramétrico de Kruskal-Wallis (α?#8197;= 5%). Resultados: a média e o desvio-padrão do teste de microdureza das amostras totais foi igual a 281,89 ± 44,51 KHN. Não houve diferença estatisticamente significativa na resistência ao cisalhamento para o fator tempo (7 ou 30 dias; F5,54= 0,105; p= 0,901). Contudo, houve diferença estatisticamente significativa para o fator solução (F5,54=6,671; p= 0,003). Essas diferenças ocorreram entre as soluções de Saliva x Coca-Cola® (p= 0,003) e Coca-Cola® x suco de limão (p= 0,029). Ao avaliar o Índice de Adesivo Remanescente, não foi possível verificar diferença significativa entre os grupos. Conclusões: o tempo de imersão utilizado nos protocolos de erosão não afetou a resistência de união dos braquetes aos dentes. A Coca-Cola® induziu valores de resistência ao cisalhamento significativamente mais altos do que o suco de limão e a saliva artificial. No entanto, os efeitos em curto prazo de 7 e 30 dias, nesse estudo in vitro, não podem ser extrapolados para os estudos in vivo. Estudos clínicos devem ser conduzidos, fundamentando os resultados laboratoriais.

Immunohistochemical study of fibronectin, tenascin and type I collagen in tongue and lip carcinomas

AIM: The aim was to compare the immunoexpression of extracellular matrix proteins in squamous cell carcinomas of tongue (SCCTo) and lower lip (SCCLi). METHODS: Eleven SCCTo and 11 SCCLi were selected and examined according to Bryne's method (1998). For immunohistochemical study utilized antibodies to fibronectin, tenascin and type I collagen. Histopathologic and immunohistochemical analysis were performed on the tumor invasive front. RESULTS: All SCCTo were classified in high score malignant grade and all SCCLi in lower score. Fibronectin showed strong immunorreactivity in the peritumoral basement membrane (BM) in 91% of SCCTo and all cases of SCCLi, while in the tumor stroma (TS) all cases of SCCTo and SCCLi had strong intensity. Tenascin had strong expression in BM of 91% cases of SCCTo and 63.4% of SCCLi and in TS had strong expression in 91% cases of SCCTo and 54.6% of SCCLi. Type I collagen demonstrated weak immunoreactivity in the TS of 72.7% cases of SCCTo and 63.4% of SCCLi. CONCLUSION: These results may suggest that the strong expression of fibronectin and tenascin proteins and the weak expression of type I collagen could play a role in the invasive process of oral SCC (AU)

The influence of non-steroidal anti-inflammatory drugs and paracetamol used for pain control of orthodontic tooth movement: a systematic review

ABSTRACT The present study aimed to perform a systematic literature review to determine if there is a non-steroidal anti-inflammatory drug (NSAID) that interferes less within tooth movement. This research was performed according to the PRISMA statement. Articles were searched in eight electronic databases (PubMed, Scopus, Embase, Web of Science, LILACS, SciELO, Google Scholar, and Open Grey). Only experimental studies on male Wistar rats were selected, which included experiments related to the influence of NSAIDs on orthodontic movement. Studies in animals with pathological conditions, literature review articles, letters to the editor and/or editorials, case reports, abstracts, books, and book chapters were excluded. Each of the steps of this systematic literature review was performed by two examiners independently. Results: the total sample consisted of 505 articles, from which 6 studies were eligible after a qualitative analysis. From the drugs assessed, paracetamol was unanimous for not interfering within orthodontic movement when compared to the control group. However, drugs such as aspirin, ibuprofen, sodium diclofenac, and selective cyclooxygenase-2 inhibitors caused a reduction in tooth movement when compared to the control group. Conclusion: paracetamol could be considered the drug of choice for pain relief because it interferes less within tooth movement.

Peri-implant assessment via cone beam computed tomography and digital periapical radiography: an ex vivo study

OBJECTIVES: This research evaluated detail registration in peri-implant bone using two different cone beam computer tomography systems and a digital periapical radiograph. METHODS: Three different image acquisition protocols were established for each cone beam computer tomography apparatus, and three clinical situations were simulated in an ex vivo fresh pig mandible: buccal bone defect, peri-implant bone defect, and bone contact. Data were subjected to two analyses: quantitative and qualitative. The quantitative analyses involved a comparison of real specimen measures using a digital caliper in three regions of the preserved buccal bone - A, B and E (control group) - to cone beam computer tomography images obtained with different protocols (kp1, kp2, kp3, ip1, ip2, and ip3). In the qualitative analyses, the ability to register peri-implant details via tomography and digital periapical radiography was verified, as indicated by twelve evaluators. Data were analyzed with ANOVA and Tukey's test (α=0.05). RESULTS: The quantitative assessment showed means statistically equal to those of the control group under the following conditions: buccal bone defect B and E with kp1 and ip1, peri-implant bone defect E with kp2 and kp3, and bone contact A with kp1, kp2, kp3, and ip2. Qualitatively, only bone contacts were significantly different among the assessments, and the p3 results differed from the p1 and p2 results. The other results were statistically equivalent. CONCLUSIONS: The registration of peri-implant details was influenced by the image acquisition protocol, although metal artifacts were produced in all situations. The evaluators preferred the Kodak 9000 3D cone beam computer tomography in most cases. The evaluators identified buccal bone defects better with cone beam computer tomography and identified peri-implant bone defects better with digital periapical radiography.

Tratamento da osteonecrose induzida por uso crônico de bifosfonatos com auxílio de protótipo tridimensional: relato de caso clínico / Treatment of osteonecrosis induced by chronic use of bisphosphonates with three-dimensional prototype of aid: a case report

Um caso clínico de tratamento de paciente com osteonecrose induzida por uso de bifosfonatos em mandíbula é apresentado neste estudo, utilizando como tratamento coadjuvante a oxigenoterapia hiperbárica e incorporando a prototipagem para o auxílio no tratamento cirúrgico da lesão, com uso de placa de reconstrução pré-modelada. Descrição do relato de caso de uma paciente, 78 anos de idade, gênero feminino, com história médica de hipertensão arterial sistêmica, diabetes, artrite e artrose, cursando com úlcera, fístula intraoral e exposição óssea e fratura patológica, associado a trauma pelo uso de prótese total. A lesão óssea foi tratada com ressecção parcial e osteossíntese de mandíbula. Seis meses após a cirurgia, não se observava clinicamente ulcerações, exposições ósseas e nem exposição da placa de reconstrução. Paciente não relatava queixas álgicas ou neurológicas. Radiograficamente não foi observado nenhum sinal de sequestro ósseo ou recidiva. Paciente encontra-se no aguardo para reconstrução óssea e reabilitação com implantes osseointegrados. Conclusão: O uso de placas reconstrutoras em cirurgia de osteossíntese de mandíbula pós-ressecção parcial, ainda é um dos melhores tratamentos indicados para osteonecrose associados a fratura patológica, favoráveis a qualidade de vida do paciente. A prototipagem tem trazido muitas vantagens como: menor morbidade, menor tempo cirúrgico, precisão na adaptação da placa ao tecido ósseo e diminuição dos custos hospitalares(AU)

A clinical case of treatment of patients with osteonecrosis induced by the use of bisphosphonates in the mandible is presented, using as adjunctive treatment the hyperbaric oxygen therapy and incorporating prototyping to aid in the surgical treatment of the lesion, using a pre-shaped reconstruction plaque. Description of the case report of a 78-year-old female patient with a medical history of systemic arterial hypertension, diabetes, arthritis and arthrosis, with ulcer, intraoral fistula and bone exposure and pathological fracture associated with Trauma due to the use of total prosthesis. The bone lesion was treated with partial resection and mandibular osteosynthesis. Six months after surgery, no ulcerations, bone exposures, or plaque exposure were clinically observed. Patient did not report pain or neurological complaints. Radiographically no signs of bone sequestration or recurrence were observed. Patient is awaiting bone reconstruction and rehabilitation with osseointegrated implants. The use of reconstructive plaques in post-resection partial mandibular osteosynthesis surgery is still one of the best treatments indicated for osteonecrosis associated with pathological fracture, favorable to patient's quality of life. Prototyping has brought many advantages such as: lower morbidity, shorter surgical time, precision in the adaptation of the plaque to the bone tissue and decrease in hospital costs(AU)

Ressecção de ameloblastoma por abordagem intra-oral, vantagens e desvantagens através da apresentação de um caso clínico / Ameloblastoma of resection in approach intra-oral, advantages and disadvantages through the presentation of a case

O objetivo desde trabalho é relatar e discutir um caso de um paciente com ameloblastoma multicístico mandibular que compareceu ao ambulatório do Serviço UFBA/ OSID de Cirurgia e Traumatologia BucoMaxiloFacial cuja ressecção e reconstrução foi realizada por via intra-oral em um tempo único, comentando as vantagens e desvantagens dessa opção terapêutica. Paciente foi encaminhada ao centro cirúrgico para ressecção do ameloblastoma sob anestesia geral. Foi realizada a ressecção parcial da mandíbula com 01cm de margem de segurança. O acesso à lesão foi todo realizado por intra-oral. Procedeu-se a ressecção do tumor, o defeito ósseo foi de aproximadamente 5 cm. A placa de reconstrução foi instalada, reestabelecendo a correta relação insterfragmentos e intermaxilares, e na região do defeito foi interposto um enxerto ósseo livre em bloco tricortical removido da crista ilíaca direita para reconstrução imediata. Conclusão: A abordagem intra-oral para tratamento e reconstrução de neoplasias benignas em região mandibular é uma forma segura e confiável, tendo como principais vantagens à ausência de cicatriz, integridade do nervo facial. E como desvantagens a contaminação por conta das secreções bucais, a infecção do enxerto levando a perda de todo o osso além de ser uma abordagem difícil requerindo habilidade do cirurgião,quando comparado com a abordagem extra-oral, sendo uma técnica bem indicada em casos selecionados(AU)

The aim of this work is to report and discuss a case of a patient with ameloblastoma mandibular multicystic who attended the outpatient clinic of UFBA Service / OSID of Oral and Maxillofacial Surgery whose resection and reconstruction was performed by intraorally in a single time, commenting on the advantages and disadvantages of this therapeutic option. The patient was referred to the operating room for resection of ameloblastoma under general anesthesia. partial resection of the mandible with 01cm margin of safety was performed. Access the injury was all done by intraoral. Proceeded to tumor resection, the bone defect was approximately 5 cm. The reconstruction plate was installed, reestablishing the correct insterfragmentos and intermaxillary relationship, and the defect region was brought a free bone graft in tricortical block removed from the right iliac crest for immediate reconstruction. Conclusion: The intraoral approach to treatment and reconstruction of benign neoplasms in mandibular region is a safe and reliable way, the main advantage of the absence of scarring, facial nerve integrity. The disadvantages contamination because of oral secretions, infection of the graft leading to loss of the entire bone and is a difficult approach requerindo surgeon's skill, compared to the extra-oral approach, being a technique indicated in selected cases(AU)

Perception of caregivers on health and oral hygiene care of institutionalized impaired elderly / Percepción de cuidadores sobre salud y cuidados de higiene oral de ancianos discapacitados institucionalizados

This study assessed the perception of caregivers on oral health of institutionalized impaired elderly, and the care provided for them in oral hygiene and prosthetic rehabilitation. A descriptive observational study was developed with caregivers of 32 institutionalized impaired elderly, in the state of Sergipe, Brazil. Data collection was performed by interviewing the caregivers, researching records of the Institution, and by clinical examination of the oral cavity of the elderly during a period between the months of March and May, 2013. Data were analyzed by descriptive statistics of absolute frequency (n) and relative frequency (%). All of the elderly presented partial (50 %) or total (50 %) tooth losses, and only 28.1 % wore removable denture. The prosthetic devices and remaining teeth of the elderly were cleaned with only toothbrush and toothpaste (100 %), with different daily frequencies among caregivers and no dentist instruction. The majority of caregivers was satisfied with the oral hygiene of the elderly (75 %) and the oral hygiene method (96.9 %) used, and showed no interest in learning new methods (81.3 %). The caregivers have an inadequate perception on oral health of the elderly population investigated, and oral hygiene care provided to the elderly is precarious.

Este estudio analizó la percepción de cuidadores sobre la salud oral de ancianos discapacitados asilados y los cuidados entregados en la higienización oral y frente a la rehabilitación protética. Se realizó un estudio observacional descriptivo, por medio de entrevistas con los respectivos cuidadores de los 32 ancianos seleccionados, institucionalizados en el Estado de Sergipe, Brasil. Todos los ancianos presentaban pérdidas dentales parciales (un 50 %) o totales (un 50 %) y solo el 28,1 % hacía uso de prótesis dentaria removible. Los aparatos protéticos y dientes remanecientes de los ancianos se higienizaban solo con cepillo y crema dental (un 100 %), con frecuencias diarias divergentes entre los cuidadores, sin la orientación de un dentista. La mayoría de los cuidadores se mostró satisfechos con la salud oral del anciano (un 75 %) y (un 96,9 %) con el método de higiene oral que utilizaban, y no expresaron interés en conocer nuevos métodos (un 81,3 %). Se concluyó que los cuidadores tienen una percepción inadecuada sobre la salud oral de la población de ancianos investigada y que los cuidados de higiene oral entregados a los ancianos son precarios.

Perception of laypersons and dentists regarding esthetic facial changes: a systematic review / Percepção estética de leigos e cirurgiões-dentistas em relação às alterações faciais: uma revisão sistemática

The need for greater understanding of subjective standards of beauty is important when combining a successful dental plan with the real needs of patients. This study aimed to perform a systematic review to assess whether dentists are more critical in assessing facial esthetics than laypersons. The cross-sectional studies selected compared dentists and laypersons regarding the perception of facial changes through photographs. The survey was conducted in four databases (PubMed, SciELO, LILACS, and OpenGrey) with no restriction of time or language, and was assessed by two reviewers who were based on the PVO strategy. Then, a manual search through the references of the selected articles was performed in order to find surveys that did not fit in said search strategy. The examiners assessed the risk of bias and quality in individual studies. The process of data synthesis was performed through a descriptive analysis of the selected studies. Three relevant articles were selected from an initial total of 979 articles collected. Most studies found that dentists were more critical than laypersons; however, facial modifications were different. Only one survey manipulated the changes on a computer, while the others worked with changes on the patient. Given the methodological diversity of the studies, it was not possible to establish a parameter to validate the results presented.

A necessidade de uma maior compreensão dos padrões subjetivos da beleza é importante quando se combina um plano odontológico de sucesso com as necessidades reais dos pacientes. Este estudo teve como objetivo realizar uma revisão sistemática para avaliar se dentistas são mais exigentes na avaliação da estética facial do que os leigos. Os estudos transversais selecionados compararam dentistas e leigos em relação à percepção de alterações faciais através de fotografias. A pesquisa foi realizada em quatro bases de dados (PubMed, SciELO, LILACS e OpenGrey), sem restrição de tempo ou de idioma, e foi realizada por dois revisores que utilizaram como referência a estratégia PVO. Em seguida, foi realizada uma busca manual através das referências dos artigos selecionados, a fim de encontrar pesquisas que não se encaixavam na referida estratégia de busca. Os examinadores avaliaram o risco de viés e qualidade nos estudos individuais. O processo de síntese dos dados foi realizado através de uma análise descritiva dos estudos selecionados. Três artigos relevantes foram selecionados de um total inicial de 979 artigos coletados. A maioria dos estudos revelou que os dentistas eram mais críticos do que os leigos; no entanto, as modificações faciais eram diferentes. Apenas uma pesquisa manipulou as alterações em um computador, enquanto as outras trabalharam com mudanças no paciente. Diante da diversidade metodológica dos estudos, não foi possível estabelecer um parâmetro para validação dos resultados apresentados.

Epidemiological Profile and Characterization of Oral and Maxillofacial injuries in Women Victims of Interpersonal Violence / Perfil Epidemiológico y Caracterización de las Lesiones Orales y Maxilofaciales en Mujeres Víctimas de Violencia Interpersonal

The objective of this study was to determine the epidemiological profile of women victims of violence and characterize the injuries affecting their maxillofacial region. This was an analytic study using secondary data from 470 expert reports of domestic violence involving women, collected from January 2007 to December 2011 in the Department of Dentistry of the Institute of Legal Medicine in the city of Feira de Santana, Bahia, Brazil. The majority of the examined women were Afro-caucasian (72.6 %), single (74.9%), and with mean age of 30.4 years. The buccal (51 %) and orbital (8.8 %) regions were the most affected extraoral sites. As to the oral cavity, the most affected sites were teeth (50.8 %) and the lip mucosa (35 %). In most cases the lesions against women were associated with an aggressor having a close relationship with the victim. The most prevalent lesions were edema; fracture; tooth dislocation; and bruise. These findings can subsidize the development of policies to prevent violence against women, and for public safety.

El objetivo fue determinar el perfil epidemiológico de mujeres víctimas de violencia y las lesiones que les acometieron la región oral y maxilofacial. Se trata de un estudio sobre datos secundarios realizado a partir del análisis de 470 informes periciales de violencia doméstica contra mujeres, recopilados entre enero del 2007 a diciembre del 2011, en el área de Odontología del Instituto Médico Legal de Feira de Santana (Bahía/Brasil). La mayoría de las mujeres estudiadas eran afrocaucásicas (72,6 %), solteras (74,9 %), con un promedio de edad de 30,4 años. En relación a la localización extraoral, las regiones bucal (51 %) y orbital (8,8 %) fueron las más afectadas. Los dientes (50,8 %) y la mucosa labial (35 %) fueron las estructuras intraorales más afectadas. En la mayoría de los casos, las lesiones estaban asociadas a un agresor con una relación cercana a las mujeres. Las lesiones más prevalentes fueron edemas, fracturas, luxación dental y equimosis. Estas hallazgos pueden colaborar en el desarrollo de acciones para combatir la violencia contra la mujer y para la seguridad pública.

Assessment of intracanal medications cytotoxicity on L929 fibroblast cells / Avaliação da citotoxicidade de medicamentos intracanais em fibroblastos L929

This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin ® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 105 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P<0.001). At 48 hours, only the OT group was cytotoxic (P <0.001). At 72 hours and at 5 days, the most cytotoxic groups were CW and OT. At 7 days, the IW and CPG groups were the least cytotoxic (P <0.001). With respect to experimental time, significant differences between 24 hours and 7 days were observed in all groups. Otosporin® was the most cytotoxic medication, followed by calcium hydroxide with distilled water.

Este estudo teve como objetivo avaliar a citotoxicidade de medicações intracanais em células L929 de fibroblastos em diferentes períodos de observação. Os seguintes grupos experimentais foram estudados: hidróxido de cálcio com paramonoclorofenol canforado e glicerina (CPG); iodofórmio com glicerina (IG); hidróxido de cálcio com iodofórmio e água destilada (CIW); iodofórmio com água destilada (IW); hidróxido de cálcio com água destilada (CW); Otosporin® (OT); e um grupo controle composto por células e meio de cultura. Os eluatos foram preparados a partir de cada grupo e colocados em contato com 1 x 105 células/poço, por períodos de 30 minutos, 12, 24, 48 e 72 horas, 5 e 7 dias. Depois de cada período experimental, um teste de citotoxicidade foi realizado utilizando metiltetrazólio (MTT) e um espectrofotômetro a uma densidade óptica de 570 nm para analisar a viabilidade celular. A análise de variância e o teste de Tukey com nível de significância de 5% foi utilizado para analisar os dados. Em 30 minutos e em 12 horas, todos os grupos foram iguais ao grupo controle. Em 24 horas, houve uma maior citotoxicidade no grupo IG do que no grupo controle (P<0,001). Em 48 horas, apenas o grupo OT foi citotóxico (P<0,001). Em 72 horas e em 5 dias, os grupos mais citotóxicos foram CW e OT. Aos 7 dias, os grupos IW e CPG foram os menos citotóxicos (P<0,001). Com relação ao tempo experimental, foram observadas diferenças significativas entre 24 horas e 7 dias em todos os grupos. Conclusão: Otosporin® foi o medicamento mais citotóxico, seguido de hidróxido de cálcio com água destilada.