RESUMEN
Abstract Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi. To optimize biosurfactant production, a PlackettBurman design and a central composite rotational design were used. The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose. The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry. F. fujikuroi reduced surface tension from 72 to 20 mN m1 under the optimized conditions of pH 5.0, 37 °C and 7 days of incubation with 190 rpm agitation. The partial identification of the structure of the biosurfactant demonstrated the presence of an ,-trehalose. The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.
RESUMEN
Abstract Biosurfactants have many advantages over synthetic surfactants but have higher production costs. Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost. The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule. The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi. To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used. The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose. The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry. F. fujikuroi reduced surface tension from 72 to 20 mN m−1 under the optimized conditions of pH 5.0, 37 °C and 7 days of incubation with 190 rpm agitation. The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose. The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.
Asunto(s)
Tensoactivos/metabolismo , Trehalosa/metabolismo , Microbiología Industrial/métodos , Fusarium/metabolismo , Tensoactivos/química , Temperatura , Medios de Cultivo/metabolismo , Fermentación , Fusarium/crecimiento & desarrollo , Fusarium/química , Concentración de Iones de HidrógenoRESUMEN
In this research it was evaluated the production of biobutanol by
Neste trabalho, foi avaliada a produção de biobutanol por
RESUMEN
The family Brassicaceae has been very studied due to the pharmacologic properties of the glucosinolates (GLS) and their hydrolysis products, which are associated with the action of an endogenous thioglucosidase myrosinase. Factors such as climate, soil, genotype, seasonal variation, processing, extraction quantification can affect the enzyme activity and stability, leading to increase or decrease the hydrolysis of GLS. Based on this aspect, the main objective of this work is present a review concerning the glucosinolate-myrosinase system, influence of climate and genotype to seasonal variation in the glucosinolate-myrosinase system, effect of thermal and high hydrostatic pressure treatments on the GLS content, as well as, the isolation and quantification of GLS from Brassica.