Aislamiento de Acremonium spp. en ulcera de cornea en un paciente con Lupus eritematoso sistemico (LES) / Annual reports 1995 / Acremonium spp in corneal ulcer in patient with systemic Lupus erythematosus (SLE)

Se presenta el caso de una paciente con ùlcera central en O.I., de 8 dìas de evoluciòn, con blefarospasmo, lagrimeo, ojo colorado. La ùlcera abarca todo el espesor de la còrnea y en una superficie de 1-1,5mm; iris y pupila no se ven. Se observan escoriaciones de rascado en ambos ojos; la paciente no refiere traumatismo pero sì automedicaciòn con colirio de cortisona; es portadora de una colagenopatìa con diagnòstico de Lupus Eritematoso Sistèmico (LES) y refiere tratamiento con prednisona 50 mg. Por referencias bibliogràficas, sabemos que los pacientes lùpicos son màs susceptibles a contraer infecciones oportunistas debidas a hongos, paràsitos y virus como efecto del tratamiento a largo plazo de corticoides, lo que va reduciendo sus defensas naturales. A la toma de muestra en el examen microscòpico directo (400X) se observaron esporos micòticos (+) y filamentos (++). El cultivo para aislamiento de bacterias en medios selectivos, Agar chocolate y thioglycolate lìquido en 5 dìas de incubaciòn a 37o.C fue negativo; sin embargo, dentro de los 15 dìas de incubaciòn a 28o.C en medios selectivos para hongos como Biggy agar y Sabouraud agar desarrolaron colonias de hongos caracterìsticas e identificadas: Acremonium spp. El tratamiento se diò a travès del Servicio de Oftalmologìa del Hospital de Clìnicas con Miconazol pomada y luego Ketoconazol gotas bebibles y tòpico, los cuales no lograron evitar la perforaciòn de la còrnea. Cuando se tratan pacientes inmunodefiecientes, se debe considerar lo susceptibilidad a infecciones oportunistas, a fin de evitar el serio problema de la automedicaciòn en estos pacientes.

Aislamiento de Acremonium spp. en ulcera de cornea en un paciente con Lupus eritematoso sistemico (LES) / Annual reports 1995 / Acremonium spp in corneal ulcer in patient with systemic Lupus erythematosus (SLE)

Se presenta el caso de una paciente con ùlcera central en O.I., de 8 dìas de evoluciòn, con blefarospasmo, lagrimeo, ojo colorado. La ùlcera abarca todo el espesor de la còrnea y en una superficie de 1-1,5mm; iris y pupila no se ven. Se observan escoriaciones de rascado en ambos ojos; la paciente no refiere traumatismo pero sì automedicaciòn con colirio de cortisona; es portadora de una colagenopatìa con diagnòstico de Lupus Eritematoso Sistèmico (LES) y refiere tratamiento con prednisona 50 mg. Por referencias bibliogràficas, sabemos que los pacientes lùpicos son màs susceptibles a contraer infecciones oportunistas debidas a hongos, paràsitos y virus como efecto del tratamiento a largo plazo de corticoides, lo que va reduciendo sus defensas naturales. A la toma de muestra en el examen microscòpico directo (400X) se observaron esporos micòticos (+) y filamentos (++). El cultivo para aislamiento de bacterias en medios selectivos, Agar chocolate y thioglycolate lìquido en 5 dìas de incubaciòn a 37o.C fue negativo; sin embargo, dentro de los 15 dìas de incubaciòn a 28o.C en medios selectivos para hongos como Biggy agar y Sabouraud agar desarrolaron colonias de hongos caracterìsticas e identificadas: Acremonium spp. El tratamiento se diò a travès del Servicio de Oftalmologìa del Hospital de Clìnicas con Miconazol pomada y luego Ketoconazol gotas bebibles y tòpico, los cuales no lograron evitar la perforaciòn de la còrnea. Cuando se tratan pacientes inmunodefiecientes, se debe considerar lo susceptibilidad a infecciones oportunistas, a fin de evitar el serio problema de la automedicaciòn en estos pacientes

Revisión de análisis bacteriológicos en hemocultivos en niños de 0 a 10 años, en el Laboratorio de Microbiología de 1990 a 1994 / Annual reports 1994 / Review of the bacteriological results in hemocultures from children between o and 10 years, in the Microbiology Department of IICS from 1990 to 1994

Este es un estudio retrospectivo de corte transversal, descriptivo para determinar la frecuencia de patógenos oportunistas en hemocultivos de niños de 0 a 10 años, analizados en el Departamento de Microbiología del Instituto de Investigaciones en Ciencias de la Salud. En número total de 58 casos y de los cuales 32 casos se analizaron con 2 muestras de sangre extraídas por médicos residentes del Servicio Pediátrico e inoculándose "in situ" en los medios de BHI y de Thioglycollate medium con SPSS en tubos de 20 c.c. para 2 c.c. de sangre total. En 12 casos se aisla: Staphylococcus coagulasa(-), siendo 100 por ciento meticilino resistentes en lactantes, y 50 por ciento en niños. Otras bacterias aisladasPseudomonas aeruginosa, Enterobacter aerógenes, Acinetobacter calcocaceticus, Klebsiella pneumoniae, Citrobacter freundii, Escherichia coli, Pseudomonas cepacia y Pseudomonas maltophylia; éstas presentan características de multirresistencia en un 100 por ciento a los antimicrobianos usuales en medio hospitalario en recién nacidos, lactantes y niños, internados. los gérmenes mencionados son componentes de la flora normal en el hombre y/o ambiente hospitalario y además potencialmente patógenas. El riesgo del fracaso terapéutico es lo mismo en un recién nacido que en un niño de 10 años

Prevalencia de Streptococcus B hemolíticos en pacientes de alto riesgo para enfermedades de transmisión sexual / Annual Reports 1992 / Prevalence of B-hemolytic Streptococcus in high risk patients for sexually transmited disease

Two hundred and fourteen prostitutes from Asunción were examined in order to determine the prevalence of cervical infection by Streptococcus B-hemolytic. The range of age was 18 to 28 years, and 21 percent of them showed Streptococcus infection, and only 30 percent of the isolate resulted sensible to penicillin. In 10 percent of the patients any microorganism could be isolate. Other microorganisms isolate were: Gardnere vaginalis: 11 percent, trichomonas vaginalis: 1 percent, Neisseria gonorrheae: 4 percent, Candida spp.: 2 percent, Corynebacterium spp.: 18 percent, Staphylococcus aureus: 1 percent and Escherichia coli: 4 percent. In 80 percent of the samples the absence of lactobacillus was n noticed. Most of the patiens (80 percent) do nout use contraceptives

Aislamiento de bacterias aeróbicas patógenas de secreción conjuntival de niños / Isolation of pathogen aeribic bacteria from conjuntival secretion of children / Annual Reports 1992

The presence of microbial organisms in conjunctival secretions always raises the doubt of whether they are the result of contamination or a real infection. Culture records of conjunctival secretion from children, who attended the Laboratory of Microbiology of the Instituto de Investigaciones en Ciencias de la Salud and the consulting roon of the Hospital de Clinícas from January 1990 to July 1992, were analyzed to determine the most frequent infectious agenis. The most commonly microorganism foud was Staphylococcus aureus (36.4 percent of the cases), followed by Streptococcus viridans (21.2 percent), Streptococcus pneumoniae (15 percent) pseudomonas aeruginosa (9 percent) and Neisseria gonorroheae (6 peecent). Nor Haemophilus influenzae neither fungi were detected

Estudio microbiológico de una infección causada por un cáteter permanente en un niño / Annual Reports 1992 / Microbiologic study of an infection caused by a permannet catheter in a child

Microbiologic study were done on a sample isolated from the end of a catheter used in a child who had devepoped acute bacterial meningitis after several admissons to hospital. The isolate was identified as Klebsiella pneumoniae which showed a remarkable multiresistance to the most common antibiotics used in hospitals

Análisis bacteriológico de agua potable de los suburbios de Asunción / Annual Reports 1991 / Bacteriological analysis of drinking water in the suburbs of Asunción

A bacteriological study of drinking water in three peripheral neighborhood of Asunción was performed. the sample were taken at nine homes dwellers were asked a questionnaire. The sample were taken in sterile 1-liter bottles from: 1. Water kept in 50 lt-plastic buckests; 2. Directly from the tap. At the time of sample taking, several children have acute diarrhea, some of them with several days of duration. The sample were filtered through 0.45 mu Millipore membranes and the material retained on the filter was cultured on S.S agar medium and incubated at 37 ºc overnigth. On the other hand, 10 ml of the sample were cultured in alkaline peptone, pH 8.5, peptone broth and selenite borth and incubated at 37 ºc for 12 h. Subculture was performed in TCBS agar, EMB agar and Ss agar respectively. Biochemical characterization of the growing micoorganisms isolate were Salmonella ssp, Enterobacter ssp and Prosteus ssp. Six samples were negative for microorganisms

Rehidratación mixta en niños menores de 5 años con diarrea aguda / Annual Reports 1991 / Mixed Rehydration in children under 5 years with acute diarrhea

In order to demonstrate the effectiveness of mixed rehydration (intravenous and oral) in children with acute diarrhea and showing dehydration, several physical parameters were meansured at the time of delivery to the Pediatrics Department of the Instituto de Previsión Social, and during the therapy. Substantial reduction in the dehydration rate were detecte by performing this method

Estudio etiológico de la diarrea bacteriana en niños en Paraguay en 1990 / Annual Reports 1991 / An etiological study of bacterial diarrhea among infants in Paraguay in 1990

An etiological study of bacterial diarrhea in infants under five years of age in Paraguay from May to July of 1990 was done. Enteropathogenic E. coli (EPEC) strains of known serogroups were isolate from 35.8 percent of the patients with diarrhea, Salmonella spp. were isolated from 15.1 percent , enterotoxigenic E. coli (ETEC) strains were isolated from 5.7 percent, and Shigella and Campylobacter spp. were isolated. Out of 53 diarrheal patients, 34 cases (64.1 percent) had bacterial diarrheal disease. The infants under one year of age with diarrhea were predominant among impatient and outpatient children under five years of age and the total number of male diarrheal cases was greater than that of female cases. Enteropathogenic organisms were isolated from nine out of 12 water samplestaken from the Paraguay River. The major serotype (O18) of the EPEC isolated from the water samples of the Paraguay River correspond to the mayor serotype isolated from patients with diarrhea

Estudio de prevalencia de algunos agentes etiológicos de enfermedades de transmición sexual en mujeres de alto riesgo (prostitutas) / Annual Reports 1991 / Study on the prevalence of some ethiological agents of sexually transmitted disease in women at high risk (prostitutes)

The prevalence of Candida albicans, Neisseria Gonorrhoeae, and trichomonas vaginalis as some of the most important agents of sexually transmditted disease (STD), were studied in 100 women, belonging to a high risk group (protitues). Also a questionnaire were provided, in order to determine the potential risk of transmission of STD at the time of the survey. The prevalence of the infection by Candida albicans in the genital tract were 7 percent, and 8 percent and 10 percent for Neisseria gonorrhoeae and Trichomonas vaginalis, respectively. In some cases, inappropriate sexual behavior, as well as high number of sexual contacts with different clients, were detected

Estudio etiológico de infección de secrecion endocervical en un grupo de alto riesgo (prostistutas) / Annual Reports 1991 / Study on the etiology of infections of endocervical secretions in a high risk group (prostitutes)

In order to determine the incidence and the etiology of the sexually trasnmitted diseaes (STD) 500 women, belonging to a high risk group (prostitutes), were studied from August, 1989 to Octuber 1990, and bacteriological and mycological studied were performed. Also, a questionnaire comprising detailed sexual behavior were rendered. High incidence of Gardnerella vaginalis (41.4 percent), Neisseria gonorrhoeae (12.2 percent), Trichomonas vaginalis (10.2 percent) and Candida albicans (10 percent) were found, in contrast with the low level found for Lactobacillus sp. The questinnaire revealed very low utilization of contraceptives as well as condom employement