Comparison of Advanced Platelet Rich Fibrin (A-PRF) and Culture Media Conditioned Warton's Jelly (CMCWJ) on Fibroblast Cells Proliferation

Objective: To compare the potency of fibroblast cells proliferation in 12.5% and 25% Culture Media Conditioned Warton's Jelly (CMCWJ) and Advanced Platelet Rich Fibrin (A-PRF) cultured medium. Material and Methods: Fibroblast cells were divided into five groups: Group I (Control Group): serum-starved fibroblast without any treatment as a negative control; Group II: fibrolast that supplemented in 12.5% CMCWJ medium; Group III: fibrolast that supplemented 12.5% A-PRF medium; Group IV: fibrolast that supplemented 25% CMCWJ medium, and Group V: fibrolast that supplemented 25% A-PRF medium. The fibroblasts proliferation was counted by an automated cell counter. Statistical analysis was performed using One-way ANOVA and Post hoc Tamhane test was conducted to analyze the potential fibroblast proliferation differences in different concentration of CMCWJ and A-PRF group. Results: There were no significant differences in the fibroblast cell proliferation between GI and GIV, GII and GIV, GII and GIII, GII and GV, also GIV and GV. There were significant differences between GI and GII, GI and GIII, GI and GV, also GIII and GIV. Conclusion: The 12.5% CMCWJ group, 12.5% A-PRF group and 25% A-PRF group has excellent potential ability of fibroblast cells proliferation, meanwhile 25% CMCWJ group has the lowest mean potency of fibroblast cells proliferation compared to other groups. The 12.5% A-PRF Group has the highest mean of fibroblast cell proliferation amongst other groups.

Comparison of Apical Adaptation Between Several Obturation Methods: An in Vitro Study

Objective: To compare lateral compaction obturation with carrier-based gutta-percha and downpack-backfill. Material and Methods: Ninety tooth with single root canal were prepared with rotary Protaper and divided into 3 groups: Group 1 obturated with lateral heated compaction (LHC), Group 2 with carrier-based gutta-percha (CP) and Group 3 with downpack-backfill (DB). The apical one-third adaptation was determined by examining the dye penetration between obturation material and root canal wall on the horizontal cut samples. The data received was analyzed using SPSS 17 software. Chi-square statistical test was done with level of significance (α) of 0.05. Results: The DB group had the highest amount of score of 0, followed by CP group and LHC group. The DB group had 28 samples (93.3%) with score of 0, which was the largest compared to the CP and LHC group. All groups had some score 2, and score 3 and 4 were only examined in the LHC group Adaptation of the apical one-third on DB group had the best result, followed by CP and LHC group (p>0.05). Conclusion: The adaptation of apical one-third by downpack-backfill was the best among the three groups, but there was no statistically significant difference among those groups.